Alignments for a candidate for glnA in Derxia gummosa DSM 723

GapMind for Amino acid biosynthesis

Alignments for a candidate for glnA in Derxia gummosa DSM 723

Align glutamate--ammonia ligase; EC 6.3.1.2 (characterized)
to candidate WP_028312523.1 H566_RS0117970 glutamate--ammonia ligase

Query= CharProtDB::CH_024014
         (469 letters)



>NCBI__GCF_000482785.1:WP_028312523.1
          Length = 471

 Score =  675 bits (1742), Expect = 0.0
 Identities = 323/469 (68%), Positives = 385/469 (82%)

Query: 1   MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFFEEGKMFDGSSIGGWKG 60
           ++A+ V+ ++ ++EVKFVD RFTDTKGKEQHV++P    + + FE G  FDGSSI GWKG
Sbjct: 3   ITADDVMKLVKDNEVKFVDFRFTDTKGKEQHVSVPLSHFDKDKFESGHAFDGSSIAGWKG 62

Query: 61  INESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYDRDPRSIAKRAEDYLRSTG 120
           I  SDM+LMPDA+TA IDPF  ++TLI+ CD+LEP   +GYDRDPRSIAKRAE YL+S+G
Sbjct: 63  IEASDMLLMPDANTAFIDPFREETTLILTCDVLEPSDGKGYDRDPRSIAKRAEAYLKSSG 122

Query: 121 IADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWNSSTQYEGGNKGHRPAVKGGY 180
           I DT  FGPEPEFF+FD +R+G+ +SGS V I+  E +W++  +YEGGN  HRPAVKGGY
Sbjct: 123 IGDTAFFGPEPEFFIFDGVRWGADMSGSFVEIESEEASWSTGKKYEGGNLAHRPAVKGGY 182

Query: 181 FPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAGQNEVATRFNTMTKKADEIQIYK 240
           FPVPPVDS QDIRSEMCL++EQ+G+ VE HHHEVA AGQNE+ T+F+T+ ++AD  Q  K
Sbjct: 183 FPVPPVDSFQDIRSEMCLILEQIGVPVEVHHHEVAGAGQNEIGTKFSTLVERADWTQKLK 242

Query: 241 YVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLSKNGVNLFAGDKYAGLSEQALYYIG 300
           YVVHNVA  +GKTATFMPKP+ GDNGSGMH H S+ K+G NLFAGD YAGLSE ALYYIG
Sbjct: 243 YVVHNVAASYGKTATFMPKPLVGDNGSGMHVHQSVWKDGKNLFAGDGYAGLSEFALYYIG 302

Query: 301 GVIKHAKAINALANPTTNSYKRLVPGYEAPVMLAYSARNRSASIRIPVVSSPKARRIEVR 360
           G+IKHA+A+NA+ NP TNSYKRLVPG+EAPV LAYSARNRSASIRIP V+S KARRIEVR
Sbjct: 303 GIIKHARALNAITNPGTNSYKRLVPGFEAPVKLAYSARNRSASIRIPYVNSDKARRIEVR 362

Query: 361 FPDPAANPYLCFAALLMAGLDGIKNKIHPGEAMDKNLYDLPPEEAKEIPQVAGSLEEALN 420
           FPDP AN YL FAA+LMAGLDG++NKIHPGEA DKNLYDLPPEE   IP V  SL++AL 
Sbjct: 363 FPDPTANSYLAFAAMLMAGLDGVQNKIHPGEAADKNLYDLPPEEDALIPTVCSSLDQALE 422

Query: 421 ELDLDREFLKAGGVFTDEAIDAYIALRREEDDRVRMTPHPVEFELYYSV 469
            LD DREFL  GGVFTD  IDAYIAL+ E+  R RMT HP+EF++YYS+
Sbjct: 423 YLDKDREFLTKGGVFTDSMIDAYIALKMEDVTRFRMTTHPIEFDMYYSL 471


Lambda     K      H
   0.318    0.136    0.400 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 741
Number of extensions: 28
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 469
Length of database: 471
Length adjustment: 33
Effective length of query: 436
Effective length of database: 438
Effective search space:   190968
Effective search space used:   190968
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 51 (24.3 bits)

Align candidate WP_028312523.1 H566_RS0117970 (glutamate--ammonia ligase)
to HMM TIGR00653 (glnA: glutamine synthetase, type I (EC 6.3.1.2))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00653.hmm
# target sequence database:        /tmp/gapView.23671.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00653  [M=462]
Accession:   TIGR00653
Description: GlnA: glutamine synthetase, type I
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   4.3e-209  680.8   0.0   4.9e-209  680.7   0.0    1.0  1  lcl|NCBI__GCF_000482785.1:WP_028312523.1  H566_RS0117970 glutamate--ammoni


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000482785.1:WP_028312523.1  H566_RS0117970 glutamate--ammonia ligase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  680.7   0.0  4.9e-209  4.9e-209       2     462 .]       7     470 ..       6     470 .. 0.99

  Alignments for each domain:
  == domain 1  score: 680.7 bits;  conditional E-value: 4.9e-209
                                 TIGR00653   2 evlkllkeenvkfvdlrfvDikGklkkveipvseleeealeegiaFDgssveGfksieesDlllkpdpe 70 
                                               +v+kl+k+++vkfvd+rf+D+kGk+++v++p+s+++++ +e+g aFDgss+ G+k+ie sD+ll+pd++
  lcl|NCBI__GCF_000482785.1:WP_028312523.1   7 DVMKLVKDNEVKFVDFRFTDTKGKEQHVSVPLSHFDKDKFESGHAFDGSSIAGWKGIEASDMLLMPDAN 75 
                                               7899***************************************************************** PP

                                 TIGR00653  71 tlvivPfraekvlrvicdvyepvtkepyerdpRsiakraeeelk.tklgdevyfGpEaEFflfdkvefk 138
                                               t++i+Pfr+e++l+++cdv ep +++ y+rdpRsiakrae +lk +++gd+++fGpE+EFf+fd v+ +
  lcl|NCBI__GCF_000482785.1:WP_028312523.1  76 TAFIDPFREETTLILTCDVLEPSDGKGYDRDPRSIAKRAEAYLKsSGIGDTAFFGPEPEFFIFDGVRWG 144
                                               ********************************************99*********************** PP

                                 TIGR00653 139 easnssflevdseegewn..reveegnkgykikkkggYfdvepvDkakdirrelvlaleelglevevsH 205
                                                 ++ sf+e++see++w+  +++e+gn +++++ kggYf+v+pvD+ +dir+e++l+le++g+ vev+H
  lcl|NCBI__GCF_000482785.1:WP_028312523.1 145 ADMSGSFVEIESEEASWStgKKYEGGNLAHRPAVKGGYFPVPPVDSFQDIRSEMCLILEQIGVPVEVHH 213
                                               *****************9999************************************************ PP

                                 TIGR00653 206 HEvata.qaEidikfdklvkaaDeivlyKyvvknvakkhGktatFmpKplfgdngsGmHvhlslwkdge 273
                                               HEva a q+Ei++kf++lv+ aD ++++Kyvv+nva+ +GktatFmpKpl+gdngsGmHvh+s+wkdg+
  lcl|NCBI__GCF_000482785.1:WP_028312523.1 214 HEVAGAgQNEIGTKFSTLVERADWTQKLKYVVHNVAASYGKTATFMPKPLVGDNGSGMHVHQSVWKDGK 282
                                               ****999************************************************************** PP

                                 TIGR00653 274 nlfageegyagLsetalyyigGilkHakalaAltnptvnsYkRLvpGyEAPvylaysaknRsaliRiPa 342
                                               nlfag+  yagLse alyyigGi+kHa+al+A+tnp +nsYkRLvpG+EAPv+laysa+nRsa+iRiP+
  lcl|NCBI__GCF_000482785.1:WP_028312523.1 283 NLFAGDG-YAGLSEFALYYIGGIIKHARALNAITNPGTNSYKRLVPGFEAPVKLAYSARNRSASIRIPY 350
                                               ******9.************************************************************* PP

                                 TIGR00653 343 sanpkakRiEvRspDpsanpYLafaallmAgldGiknkidpgepldknlyelseeelkelGieqlpesL 411
                                               + + ka+RiEvR+pDp+an+YLafaa+lmAgldG++nki+pge+ dknly+l++ee +   i+++ +sL
  lcl|NCBI__GCF_000482785.1:WP_028312523.1 351 VNSDKARRIEVRFPDPTANSYLAFAAMLMAGLDGVQNKIHPGEAADKNLYDLPPEEDAL--IPTVCSSL 417
                                               ********************************************************999..******** PP

                                 TIGR00653 412 eealdelesdk..evlkevlgeelieafielkrkEveelrlkvhpvElekyld 462
                                               ++al+ l++d+  +++++v+++ +i+a+i+lk+++v ++r++ hp+E+ +y++
  lcl|NCBI__GCF_000482785.1:WP_028312523.1 418 DQALEYLDKDRefLTKGGVFTDSMIDAYIALKMEDVTRFRMTTHPIEFDMYYS 470
                                               ***********888999**********************************95 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (462 nodes)
Target sequences:                          1  (471 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 12.27
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis