Alignments for a candidate for dapA in Desulfatibacillum aliphaticivorans DSM 15576

GapMind for Amino acid biosynthesis

Alignments for a candidate for dapA in Desulfatibacillum aliphaticivorans DSM 15576

Align 4-hydroxy-tetrahydrodipicolinate synthase (EC 4.3.3.7) (characterized)
to candidate WP_028315051.1 G491_RS0114125 4-hydroxy-tetrahydrodipicolinate synthase

Query= BRENDA::Q9I4W3
         (292 letters)



>NCBI__GCF_000429905.1:WP_028315051.1
          Length = 344

 Score =  189 bits (479), Expect = 1e-52
 Identities = 120/294 (40%), Positives = 169/294 (57%), Gaps = 21/294 (7%)

Query: 1   MIAGSMVALVTPFDAQGRLDWDSLAKLVDFHLQEGTNAIVAVGTTGESATLDVEEHIQVI 60
           M  G   ALVTPF ++G LD   L KLV+F +  G + I+AVGTTGES TL  EEH+QVI
Sbjct: 1   MTKGCNTALVTPF-SEGGLDITGLEKLVEFQIAGGVDGILAVGTTGESPTLSWEEHLQVI 59

Query: 61  RRVVDQVKGRIPVIAGTGANSTREAVALTEAAKSGGADACLLVTPYYNKPTQ-EGMYQHF 119
           + V D+ KG++  IAGTG+N+T+E +  T+ A   G DA LLV PYYN P+  E   ++ 
Sbjct: 60  QIVADKTKGKVKCIAGTGSNNTKETLEATKHAAKAGVDAVLLVDPYYNGPSSLEIRREYL 119

Query: 120 RHIAEAVAIPQIL-YNVPGRTSCDMLPETVERLSK-VPNIIGIKEATGDLQRAKEVIERV 177
              A+A    +I+ Y +PGRT   + PE +  L K  PN+  +KEATGDL   ++  +  
Sbjct: 120 TPTAKAFPDLEIIPYVIPGRTGAQLFPEDLAILYKECPNVKTVKEATGDLDNMRKTRKLC 179

Query: 178 GKDFLVYSGDDATAVELML---LGGKGNISVTANVAPRAMSDLCAAAMRGDAAAARAIND 234
           G D+ + SGDD   +++ML   +   G ISV +NVAP+A+S + AAA  GDA  AR I  
Sbjct: 180 GPDYTIMSGDDDMTLQMMLDPDIKASGVISVLSNVAPKAVSQMTAAAAAGDADLARNIGA 239

Query: 235 RLMPLHKALFIES--------------NPIPVKWALHEMGLIPEGIRLPLTWLS 274
            L PL   + +++              NP+ VK  +  +G+     R PL  +S
Sbjct: 240 ALKPLFGLVVVKTTEESPVGPVMCRARNPLGVKTLMAALGMPCGPCRPPLGKMS 293


Lambda     K      H
   0.319    0.134    0.393 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 225
Number of extensions: 8
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 292
Length of database: 344
Length adjustment: 27
Effective length of query: 265
Effective length of database: 317
Effective search space:    84005
Effective search space used:    84005
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 48 (23.1 bits)

Align candidate WP_028315051.1 G491_RS0114125 (4-hydroxy-tetrahydrodipicolinate synthase)
to HMM TIGR00674 (dapA: 4-hydroxy-tetrahydrodipicolinate synthase (EC 4.3.3.7))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00674.hmm
# target sequence database:        /tmp/gapView.9220.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00674  [M=286]
Accession:   TIGR00674
Description: dapA: 4-hydroxy-tetrahydrodipicolinate synthase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
    5.2e-76  241.2   0.1    2.9e-75  238.8   0.1    1.8  1  lcl|NCBI__GCF_000429905.1:WP_028315051.1  G491_RS0114125 4-hydroxy-tetrahy


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000429905.1:WP_028315051.1  G491_RS0114125 4-hydroxy-tetrahydrodipicolinate synthase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  238.8   0.1   2.9e-75   2.9e-75       3     281 ..       6     302 ..       4     307 .. 0.92

  Alignments for each domain:
  == domain 1  score: 238.8 bits;  conditional E-value: 2.9e-75
                                 TIGR00674   3 ltAliTPfkedgsvdfaalekliesqiekgvdaivvvGtTGEsatLsleEkkkvievavelvknrvpvi 71 
                                                tAl+TPf e g +d++ lekl+e+qi+ gvd+i++vGtTGEs+tLs+eE+ +vi+++++ +k++v+ i
  lcl|NCBI__GCF_000429905.1:WP_028315051.1   6 NTALVTPFSEGG-LDITGLEKLVEFQIAGGVDGILAVGTTGESPTLSWEEHLQVIQIVADKTKGKVKCI 73 
                                               69*******999.******************************************************** PP

                                 TIGR00674  72 aGtgsnateeaieltkeaeklgvdgvlvvtPyYnkPtqeGlykhfka.iaeev.elPiilYnvPsRtgv 138
                                               aGtgsn+t+e++e tk+a+k+gvd+vl+v PyYn P+   + + + +  a++  +l ii Y +P+Rtg+
  lcl|NCBI__GCF_000429905.1:WP_028315051.1  74 AGTGSNNTKETLEATKHAAKAGVDAVLLVDPYYNGPSSLEIRREYLTpTAKAFpDLEIIPYVIPGRTGA 142
                                               ************************************9888877665515555579************** PP

                                 TIGR00674 139 slepetvkrLaeeve.ivaiKeasgdlervseikaeakedfkvlsGdDaltleila...lGakGviSVa 203
                                               +l pe ++ L +e++ + ++Kea+gdl+++ + ++  + d++++sGdD +tl++++   + a+GviSV+
  lcl|NCBI__GCF_000429905.1:WP_028315051.1 143 QLFPEDLAILYKECPnVKTVKEATGDLDNMRKTRKLCGPDYTIMSGDDDMTLQMMLdpdIKASGVISVL 211
                                               ************9998***************************************744468******** PP

                                 TIGR00674 204 snvapkelkemvkaalegdteeareihqkllklfkalfiet..............NPipvKtalallgl 258
                                               snvapk +++m +aa++gd + ar+i + l++lf  + ++t              NP++vKt++a+lg+
  lcl|NCBI__GCF_000429905.1:WP_028315051.1 212 SNVAPKAVSQMTAAAAAGDADLARNIGAALKPLFGLVVVKTteespvgpvmcrarNPLGVKTLMAALGM 280
                                               *********************************9988876556666666666666************** PP

                                 TIGR00674 259 iekdelRlPLtelseekkeklke 281
                                               + +  +R+PL ++s++  + + +
  lcl|NCBI__GCF_000429905.1:WP_028315051.1 281 PCG-PCRPPLGKMSKQGLDVVVN 302
                                               ***.9*********998777665 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (286 nodes)
Target sequences:                          1  (344 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.03u 0.00s 00:00:00.03 Elapsed: 00:00:00.03
# Mc/sec: 2.95
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis