Alignments for a candidate for dapC in Hydrogenovibrio halophilus DSM 15072

GapMind for Amino acid biosynthesis

Alignments for a candidate for dapC in Hydrogenovibrio halophilus DSM 15072

Align phosphoserine transaminase (EC 2.6.1.52) (characterized)
to candidate WP_028485474.1 A377_RS0110550 3-phosphoserine/phosphohydroxythreonine transaminase

Query= BRENDA::P23721
         (362 letters)



>NCBI__GCF_000384235.1:WP_028485474.1
          Length = 354

 Score =  383 bits (984), Expect = e-111
 Identities = 197/358 (55%), Positives = 245/358 (68%), Gaps = 9/358 (2%)

Query: 5   FNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGKEFIQVAEEAEKDFRDLLNVP 64
           FNFS+GPAMLP  V+++AQ E  DW   G SVME+SHR  EF+ VA E E D R+L+ +P
Sbjct: 4   FNFSAGPAMLPEAVMQEAQAEFLDWQNTGMSVMEMSHRSPEFMSVAHEMEADLRELMAIP 63

Query: 65  SNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYWAASAIKEAKKYCTPN-VFDAKV 123
            +YKVLF HGG   QFA +P N+     T DY + G W+A AIKEA ++   N V  A+ 
Sbjct: 64  DDYKVLFTHGGASLQFAGIPFNLTQPGDTVDYCNTGVWSAKAIKEASRFVQVNEVAKAET 123

Query: 124 TVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAIDETPDFGADVVVAADFSSTILSRP 183
           T+          +WQLSDNA Y+H C NETI G+     PD   D  + ADFSSTILSRP
Sbjct: 124 TIPDFD------QWQLSDNAKYLHICQNETITGVEFQSLPD--TDKPIIADFSSTILSRP 175

Query: 184 IDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKANIACPSILDYSILNDNGSMFNTPPT 243
           I+V+ YG+IYAGAQKNIGPAGL IVIVREDL+G A    P+++++   +   SMFNTP T
Sbjct: 176 INVADYGLIYAGAQKNIGPAGLAIVIVREDLIGHARETTPTLMNWQTYDQFESMFNTPAT 235

Query: 244 FAWYLSGLVFKWLKANGGVAEMDKINQQKAELLYGVIDNSDFYRNDVAKANRSRMNVPFQ 303
           FAWYL+G VFKWLKA GG+  M  +N++KA+ LY +ID S  Y N+VA + RS MNV F+
Sbjct: 236 FAWYLAGKVFKWLKAQGGLDAMATLNERKAQKLYTLIDRSGLYYNEVAPSVRSWMNVTFK 295

Query: 304 LADSALDKLFLEESFAAGLHALKGHRVVGGMRASIYNAMPLEGVKALTDFMVEFERRH 361
           L D  L++ FL ES  AGL +L+GH+  GGMRASIYNAMP  GV AL DFM  FE+RH
Sbjct: 296 LKDETLNQAFLTESREAGLLSLRGHKTYGGMRASIYNAMPEAGVDALVDFMQAFEQRH 353


Lambda     K      H
   0.319    0.136    0.405 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 394
Number of extensions: 20
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 362
Length of database: 354
Length adjustment: 29
Effective length of query: 333
Effective length of database: 325
Effective search space:   108225
Effective search space used:   108225
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 49 (23.5 bits)

Align candidate WP_028485474.1 A377_RS0110550 (3-phosphoserine/phosphohydroxythreonine transaminase)
to HMM TIGR01364 (serC: phosphoserine transaminase (EC 2.6.1.52))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01364.hmm
# target sequence database:        /tmp/gapView.10230.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01364  [M=358]
Accession:   TIGR01364
Description: serC_1: phosphoserine transaminase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   2.2e-152  493.0   0.0   2.4e-152  492.9   0.0    1.0  1  lcl|NCBI__GCF_000384235.1:WP_028485474.1  A377_RS0110550 3-phosphoserine/p


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000384235.1:WP_028485474.1  A377_RS0110550 3-phosphoserine/phosphohydroxythreonine transaminase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  492.9   0.0  2.4e-152  2.4e-152       2     358 .]       4     353 ..       3     353 .. 0.98

  Alignments for each domain:
  == domain 1  score: 492.9 bits;  conditional E-value: 2.4e-152
                                 TIGR01364   2 vnFsaGPaalpeevlekaqkelldfnglglsvmeisHRskefekvveeaesdlreLlnipdnyevlflq 70 
                                               +nFsaGPa+lpe+v+++aq+e+ld++++g+svme+sHRs ef++v++e e dlreL+ ipd+y+vlf  
  lcl|NCBI__GCF_000384235.1:WP_028485474.1   4 FNFSAGPAMLPEAVMQEAQAEFLDWQNTGMSVMEMSHRSPEFMSVAHEMEADLRELMAIPDDYKVLFTH 72 
                                               8******************************************************************** PP

                                 TIGR01364  71 GGattqfaavplnllkekkvadyivtGawskkalkeakkltkevkvvaseeekkyskipdeeelelked 139
                                               GGa+ qfa +p+nl++ ++++dy +tG+ws+ka+kea+++++ v+ va++e    ++ipd ++++l+++
  lcl|NCBI__GCF_000384235.1:WP_028485474.1  73 GGASLQFAGIPFNLTQPGDTVDYCNTGVWSAKAIKEASRFVQ-VNEVAKAE----TTIPDFDQWQLSDN 136
                                               ****************************************98.88888885....78************ PP

                                 TIGR01364 140 aayvylcanetieGvefkelpevkkaplvaDlssdilsrkidvskygliyaGaqKniGpaGvtvvivrk 208
                                               a+y+++c+neti+Gvef++lp  +++p++aD+ss ilsr+i+v++ygliyaGaqKniGpaG+++vivr+
  lcl|NCBI__GCF_000384235.1:WP_028485474.1 137 AKYLHICQNETITGVEFQSLP-DTDKPIIADFSSTILSRPINVADYGLIYAGAQKNIGPAGLAIVIVRE 204
                                               *********************.77899****************************************** PP

                                 TIGR01364 209 dllerakkelpsvldYkilaendslyntpptfaiyvlglvlkwlkekGGvkklekknqeKakllYeaid 277
                                               dl+++a++++p+++++++  + +s++ntp+tfa+y++g+v+kwlk++GG+++++  n++Ka+ lY +id
  lcl|NCBI__GCF_000384235.1:WP_028485474.1 205 DLIGHARETTPTLMNWQTYDQFESMFNTPATFAWYLAGKVFKWLKAQGGLDAMATLNERKAQKLYTLID 273
                                               ********************************************************************* PP

                                 TIGR01364 278 esegfyknkvekkaRslmnvvFtlkkeelekeFlkeaeekglvslkGhrsvGGiRasiYnalpleevqa 346
                                               +s g+y n+v+++ Rs+mnv+F+lk+e l++ Fl+e++e+gl+sl+Gh++ GG+RasiYna+p ++v a
  lcl|NCBI__GCF_000384235.1:WP_028485474.1 274 RS-GLYYNEVAPSVRSWMNVTFKLKDETLNQAFLTESREAGLLSLRGHKTYGGMRASIYNAMPEAGVDA 341
                                               99.6999************************************************************** PP

                                 TIGR01364 347 LvdfmkeFekkh 358
                                               Lvdfm+ Fe++h
  lcl|NCBI__GCF_000384235.1:WP_028485474.1 342 LVDFMQAFEQRH 353
                                               *********987 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (358 nodes)
Target sequences:                          1  (354 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.01
# Mc/sec: 7.61
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis