Alignments for a candidate for gatA in Thiothrix lacustris DSM 21227

GapMind for Amino acid biosynthesis

Alignments for a candidate for gatA in Thiothrix lacustris DSM 21227

Align Glutamyl-tRNA(Gln) amidotransferase subunit A; Glu-ADT subunit A; EC 6.3.5.7 (characterized)
to candidate WP_028489611.1 Q394_RS0112735 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA

Query= SwissProt::O06491
         (485 letters)



>NCBI__GCF_000621325.1:WP_028489611.1
          Length = 486

 Score =  478 bits (1229), Expect = e-139
 Identities = 227/470 (48%), Positives = 332/470 (70%), Gaps = 1/470 (0%)

Query: 15  IHKKEIKISDLVDESYKRIQAVDDKVQAFLALDEERARAYAKELDEAVDGRSEHGLLFGM 74
           +H  +  + +  D    RI+  + ++ A++ +  + A+  A ++D  +      G + G+
Sbjct: 13  VHTGKFSVMEATDAYLDRIERFNPELNAYITVTRDTAKTQAVDIDNRIRKGELTGAMAGV 72

Query: 75  PIGVKDNIVTKGLRTTCSSKILENFDPIYDATVVQRLQDAEAVTIGKLNMDEFAMGSSTE 134
           P  +KD   ++G+ TTC+S +L NF   YDA V ++L+ A  V +GK NMDEFAMGSS E
Sbjct: 73  PYALKDLFCSEGVLTTCASNMLANFISPYDAHVAEKLKAAGGVLLGKNNMDEFAMGSSNE 132

Query: 135 NSAYKLTKNPWNLDTVPGGSSGGSAAAVAAGEVPFSLGSDTGGSIRQPASFCGVVGLKPT 194
            SA+    NPW++  VPGGSSGG AA +AA   P +LG+DTGGSIRQPASFC + G+KPT
Sbjct: 133 TSAFGSVCNPWDVSKVPGGSSGGGAATIAARLAPMTLGTDTGGSIRQPASFCNITGIKPT 192

Query: 195 YGRVSRYGLVAFASSLDQIGPITRTVEDNAFLLQAISGVDKMDSTSANVDVPDFLSSLTG 254
           YGR+SRYG++AFASSLDQ GP+  + ED A  L  I+G+D  DST  ++ VPD+ ++L G
Sbjct: 193 YGRISRYGMIAFASSLDQCGPMAASAEDCALTLNIIAGLDGRDSTCMDLPVPDYTATLNG 252

Query: 255 DIKGLKIAVPKEYLGEGVGKEARESVLAALKVLEGLGATWEEVSLPHSKYALATYYLLSS 314
            ++GL+I +PKE+  EG+  +  + +  A+K  +  GA  +EV+LP+S  A+  YY+++ 
Sbjct: 253 SLEGLRIGLPKEFFAEGLDAQVADVIDRAIKQFQAKGAIIKEVTLPNSHLAVPVYYVVAP 312

Query: 315 SEASANLARFDGIRYGYRTDNADNLIDLYKQTRAEGFGNEVKRRIMLGTFALSSGYYDAY 374
           +E S+NL+RFDG+R+G+R +N  +L+DLY+++R EGFG+EVKRRIM+GT+ALS+GYYDAY
Sbjct: 313 AECSSNLSRFDGVRFGHRCENPKDLMDLYERSRWEGFGDEVKRRIMIGTYALSAGYYDAY 372

Query: 375 YKKAQKVRTLIKKDFEDVFEKYDVIVGPTTPTPAFKIGENTKDPLTMYANDILTIPVNLA 434
           Y KAQ+VR LIK+DFE  F+  DVI+GP+ PT AF IGE   DP++MY  D+ TIPV+LA
Sbjct: 373 YLKAQQVRRLIKQDFEAAFKDVDVIMGPSCPTTAFGIGEKKDDPISMYLEDLYTIPVSLA 432

Query: 435 GVPGISVPCGL-ADGLPLGLQIIGKHFDESTVYRVAHAFEQATDHHKAKP 483
           G+PG++ P G  ADGLP+G+Q++G +F+E+ +  +AH ++Q TD H   P
Sbjct: 433 GLPGMTFPIGFAADGLPVGMQLVGNYFEEARMLNIAHQYQQWTDWHTQVP 482


Lambda     K      H
   0.315    0.134    0.381 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 599
Number of extensions: 26
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 485
Length of database: 486
Length adjustment: 34
Effective length of query: 451
Effective length of database: 452
Effective search space:   203852
Effective search space used:   203852
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 52 (24.6 bits)

Align candidate WP_028489611.1 Q394_RS0112735 (Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA)
to HMM TIGR00132 (gatA: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, A subunit (EC 6.3.5.-))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00132.hmm
# target sequence database:        /tmp/gapView.21341.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00132  [M=466]
Accession:   TIGR00132
Description: gatA: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, A subunit
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   5.3e-186  604.8   0.0     6e-186  604.6   0.0    1.0  1  lcl|NCBI__GCF_000621325.1:WP_028489611.1  Q394_RS0112735 Asp-tRNA(Asn)/Glu


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000621325.1:WP_028489611.1  Q394_RS0112735 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  604.6   0.0    6e-186    6e-186       4     465 ..      12     476 ..       9     477 .. 0.98

  Alignments for each domain:
  == domain 1  score: 604.6 bits;  conditional E-value: 6e-186
                                 TIGR00132   4 llkkkevsikevleeilerieavkdkinaflevtkekalkkakkldkkva.ke.k.klagipiavKdni 69 
                                                +++ ++s+ e+++++l+rie+++ ++na+++vt+++a ++a  +d++++  e +  +ag+p+a+Kd +
  lcl|NCBI__GCF_000621325.1:WP_028489611.1  12 GVHTGKFSVMEATDAYLDRIERFNPELNAYITVTRDTAKTQAVDIDNRIRkGElTgAMAGVPYALKDLF 80 
                                               577889999****************************************97445569************ PP

                                 TIGR00132  70 avkdiettcaSkiLenyvspydatVverlkeagaliiGktNlDEFamGsstetSafgvtknPeneervp 138
                                               + +++ ttcaS++L n++spyda+V e+lk+ag +++Gk N+DEFamGss etSafg + nP++ ++vp
  lcl|NCBI__GCF_000621325.1:WP_028489611.1  81 CSEGVLTTCASNMLANFISPYDAHVAEKLKAAGGVLLGKNNMDEFAMGSSNETSAFGSVCNPWDVSKVP 149
                                               ********************************************************************* PP

                                 TIGR00132 139 GGSsgGsaaavaadlvplalgsDTGgSiRqPAsfcgvvGlKPtYGlvSRyGlvayasSldqiGilakkv 207
                                               GGSsgG aa++aa l+p++lg+DTGgSiRqPAsfc++ G+KPtYG++SRyG++a+asSldq G++a++ 
  lcl|NCBI__GCF_000621325.1:WP_028489611.1 150 GGSSGGGAATIAARLAPMTLGTDTGGSIRQPASFCNITGIKPTYGRISRYGMIAFASSLDQCGPMAASA 218
                                               ********************************************************************* PP

                                 TIGR00132 208 edialvldvisgkDkkDstslevkveelleelkkdlkglkvgvvkelseesldkevkekfeklleklee 276
                                               ed al l++i+g D +Dst ++ +v++++ +l+  l gl++g+ ke++ e+ld +v++ ++++++++++
  lcl|NCBI__GCF_000621325.1:WP_028489611.1 219 EDCALTLNIIAGLDGRDSTCMDLPVPDYTATLNGSLEGLRIGLPKEFFAEGLDAQVADVIDRAIKQFQA 287
                                               ********************************************************************* PP

                                 TIGR00132 277 lgaeivevslpsvklalaiYyiispsEassnlarydgiryGkrveelkslkelyaktRsegfgeevkrR 345
                                               +ga i+ev lp+++la+++Yy+++p+E ssnl+r+dg+r+G+r e++k+l++ly ++R egfg+evkrR
  lcl|NCBI__GCF_000621325.1:WP_028489611.1 288 KGAIIKEVTLPNSHLAVPVYYVVAPAECSSNLSRFDGVRFGHRCENPKDLMDLYERSRWEGFGDEVKRR 356
                                               ********************************************************************* PP

                                 TIGR00132 346 imlGayalskeyydkyykkAqkvrtliidefeklfeevDvivsptaptlafklgekaedplemylsDvl 414
                                               im+G+yals++yyd+yy+kAq+vr+li+++fe +f++vDvi++p+ pt+af +gek++dp++myl+D++
  lcl|NCBI__GCF_000621325.1:WP_028489611.1 357 IMIGTYALSAGYYDAYYLKAQQVRRLIKQDFEAAFKDVDVIMGPSCPTTAFGIGEKKDDPISMYLEDLY 425
                                               ********************************************************************* PP

                                 TIGR00132 415 tvpanlaGlpaisvPlgkkekglpiGlqiigkafddkkllsvakaleqald 465
                                               t+p++laGlp+++ P+g + +glp+G+q++g++f++ ++l++a++++q +d
  lcl|NCBI__GCF_000621325.1:WP_028489611.1 426 TIPVSLAGLPGMTFPIGFAADGLPVGMQLVGNYFEEARMLNIAHQYQQWTD 476
                                               **********************************************99765 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (466 nodes)
Target sequences:                          1  (486 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.02u 0.00s 00:00:00.02 Elapsed: 00:00:00.02
# Mc/sec: 11.20
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis