Align Shikimate kinase; SK; EC 2.7.1.71 (uncharacterized)
to candidate WP_028583259.1 G494_RS0102485 shikimate kinase
Query= curated2:Q8RF94 (172 letters) >NCBI__GCF_000429965.1:WP_028583259.1 Length = 170 Score = 87.4 bits (215), Expect = 1e-22 Identities = 50/151 (33%), Positives = 87/151 (57%), Gaps = 4/151 (2%) Query: 1 MKDNIALIGFMGSGKTTVGKLLAKTMDMKFVDIDKVIEAHEKKSINDIFHEKGQIYFRDL 60 MK N+ LIG G+GK+T+G +LAK + + F+D D +I+ ++++S+ +I ++ R + Sbjct: 1 MKTNLTLIGMPGAGKSTIGIILAKNLSLGFIDTDVLIQINQQRSLQEIIDASDYLHLRQV 60 Query: 61 EREIILQESLKNDCVIATGGGSILDNENIKRLKETSFIVFLNATIECLYLRLKDNTTRPI 120 E + I + +L N VIATGG + ++ L SFIVFL + L R+ + TR I Sbjct: 61 EADEISKLNLSNH-VIATGGSAAYSPRAMEHLARISFIVFLEVSFAELNRRITNFETRGI 119 Query: 121 LNDVEDKRKLIEELLEKRKFLYQISADYIID 151 + + ++L ++R+ LY+ AD +D Sbjct: 120 ---AKADHQSFQDLFDERQLLYRRYADITLD 147 Lambda K H 0.320 0.140 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 64 Number of extensions: 2 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 172 Length of database: 170 Length adjustment: 18 Effective length of query: 154 Effective length of database: 152 Effective search space: 23408 Effective search space used: 23408 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 43 (21.2 bits)
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory