Annotation: NCBI__GCF_000423825.1:WP_028988857.1
Length: 786 amino acids
Source: GCF_000423825.1 in NCBI
Pathway | Step | Score | Similar to | Id. | Cov. | Bits | Other hit | Other id. | Other bits |
---|---|---|---|---|---|---|---|---|---|
L-methionine biosynthesis | metE | hi | 5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase (EC 2.1.1.14) (characterized) | 52% | 99% | 765 | |||
L-methionine biosynthesis | split_metE_2 | lo | Predicted split_metE_2 by GapMind curators (no experimental data) | 34% | 96% | 182.2 | 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase; Cobalamin-independent methionine synthase; Pollen allergen Sal k 3; SkMetE; Vitamin-B12-independent methionine synthase; Allergen Sal k 3.0101; EC 2.1.1.14 | 52% | 745.3 |
View WP_028988857.1 at NCBI
Find papers: PaperBLAST
Find functional residues: SitesBLAST
Search for conserved domains
Find the best match in UniProt
Compare to protein structures
Predict transmenbrane helices: Phobius
Predict protein localization: PSORTb
Find homologs in fast.genomics
Fitness BLAST: loading...
MTLTHNLGFPRIGQRRELKKALESFWKGEIDESTLRETGRALRAKHWQIQQAAGLDLIPV GDFSYYDQMLDMSALLGAIPARYGFAYGTVDFATYFAMARGDAAQPALEMTKWFDTNYHY LVPEFTAETRFQLSSTALFEAVAEAQALGVQPKPVLIGPLTYLWLGKERAAQAERAENHP PAVAGCGHSHAAPRVHAAGFDRLSLLDRLLPVYAEILARLKAQGVNWVQMDEPALVLDLP AEWLGALDRIYAALATADAPRILLTTYFGSVAEIAGQLKRLPVAGLHLDLVRASGQLEAF LADYPADKVLSLGVVDGRNVWRADLDAALSTLEAVRAVLGERLWIAPSCSLLHVPMDLEE ESGLDPELKSWLAFAVQKLDEVKILGTALDHGRESVAEPLAAARAARQGRRNSTRIHNPA VSARVAALTPGDDQRRSAFPQRQAAQRERFGLPLFPTTTIGSFPQTDAIRRARLQFRKGM IEAAAYEQAMQGEIEYAVRRQEALGLDVLVHGEAERNDMVEYFGEQLEGFSFTRHAWVQS YGSRCVKPPIIFGDVSRPRPMTVSWTRYAQSLTERPMKGMLTGPVTILQWSFVRDDQPRA ETALQIALAIRDEVRDLERAGIGIIQIDEPAYREGLPLRKKDWEDYLAWASRSFRIAASG VDDATQIHTHMCYSEFNDILPAIAAMDADVITIETSRSQMELLDAFGQFDYPNEIGPGVY DIHSPRVPATEEMIALLEKASRVVPPERLWVNPDCGLKTRKWEEVEPALRNMVDAARALR ASTRRG
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory