Alignments for a candidate for hisC in Thermithiobacillus tepidarius DSM 3134

GapMind for Amino acid biosynthesis

Alignments for a candidate for hisC in Thermithiobacillus tepidarius DSM 3134

Align histidinol-phosphate aminotransferase; tyrosine/phenylalanine aminotransferase (promiscuous) (EC 2.6.1.1; EC 2.6.1.9) (characterized)
to candidate WP_028990084.1 G579_RS0110095 histidinol-phosphate transaminase

Query= metacyc::BSU22620-MONOMER
         (360 letters)



>NCBI__GCF_000423825.1:WP_028990084.1
          Length = 371

 Score =  284 bits (726), Expect = 3e-81
 Identities = 154/351 (43%), Positives = 207/351 (58%), Gaps = 1/351 (0%)

Query: 10  LKPYQPGKPIEAVKSEYGLDKVVKLASNENPYGCSEAAKEALHHEIQQLALYPDGYSAAL 69
           LKPY+PGKPIE ++ EYG+   +KLASNENP G S  A EA+     +LALYPDG   AL
Sbjct: 20  LKPYEPGKPIETLEREYGVRNAIKLASNENPLGPSPLALEAVIKASAKLALYPDGAGFAL 79

Query: 70  RTRLSKHLNVSETSLIFGNGSDEIIQIICRAFLNDKTNTVTAAPTFPQYKHNAVIEGAEV 129
           +  L +HL V    ++ GNGS+EI++++ R F+      V +   F  Y       GA  
Sbjct: 80  KAALGRHLGVQPEQVVLGNGSNEILELVTRTFVTPADEVVFSEHAFAVYPIVTQAVGATA 139

Query: 130 REIALRPDGSHDLDAMLEAIDEQTQVVWICSPNNPTGTYTSEGELLAFLERVPSRVLVVL 189
           R +A   D  HDLDAM   ++E T++V++ +PNNPTGTY +   L AF+  +PS VLVVL
Sbjct: 140 R-VAPARDYGHDLDAMAGLVNENTRLVFVANPNNPTGTYLAREALEAFITSMPSHVLVVL 198

Query: 190 DEAYYEYVTAEDYPETVPLLSKYSNLMILRTFSKAYGLAALRVGYGIADENLIRQIEPAR 249
           DEAY+E+V A DYP+ +  L ++ NL++ RTFSK YGLA LRVGYG+    LI  +E  R
Sbjct: 199 DEAYFEFVDAPDYPDGIAWLERFDNLLVTRTFSKIYGLAGLRVGYGVGHPKLISLLERVR 258

Query: 250 EPFNTSRLGQAAAIAALDDQAFIASCVEQNNAGLQQYYDFAKTHGLKCYPSQTNFVLIDF 309
           +PFN +    AAA AAL D   +      N +G+ Q     +  GL   PS  NF+ +  
Sbjct: 259 QPFNVNSAAMAAAEAALADHEHLRRSRALNASGMAQLVAGIEALGLHALPSVGNFLAVHV 318

Query: 310 KRPADELFQALLEKGYIVRSGNALGFPTSLRITIGTKEQNEEILAILAEIL 360
            R A  +++ALL +G IVR     G P  LR+TIG  E+N   L  L   L
Sbjct: 319 GRDAAPVYEALLRQGVIVRPVAGYGMPEFLRVTIGRPEENARFLQALEAAL 369


Lambda     K      H
   0.317    0.134    0.382 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 388
Number of extensions: 13
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 360
Length of database: 371
Length adjustment: 30
Effective length of query: 330
Effective length of database: 341
Effective search space:   112530
Effective search space used:   112530
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 49 (23.5 bits)

Align candidate WP_028990084.1 G579_RS0110095 (histidinol-phosphate transaminase)
to HMM TIGR01141 (hisC: histidinol-phosphate transaminase (EC 2.6.1.9))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01141.hmm
# target sequence database:        /tmp/gapView.11881.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01141  [M=349]
Accession:   TIGR01141
Description: hisC: histidinol-phosphate transaminase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   6.7e-112  359.7   0.0   7.6e-112  359.6   0.0    1.0  1  lcl|NCBI__GCF_000423825.1:WP_028990084.1  G579_RS0110095 histidinol-phosph


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000423825.1:WP_028990084.1  G579_RS0110095 histidinol-phosphate transaminase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  359.6   0.0  7.6e-112  7.6e-112       4     347 ..      17     366 ..      14     368 .. 0.98

  Alignments for each domain:
  == domain 1  score: 359.6 bits;  conditional E-value: 7.6e-112
                                 TIGR01141   4 ikklepYqpg......arelgekevvkLnsnEnPfgpsekvkealkeelkklhrYpdpqalelkealak 66 
                                               +  l+pY+pg      +re+g  + +kL+snEnP+gps+ ++ea+ ++ +kl +Ypd  +++lk+al +
  lcl|NCBI__GCF_000423825.1:WP_028990084.1  17 VAGLKPYEPGkpietlEREYGVRNAIKLASNENPLGPSPLALEAVIKASAKLALYPDGAGFALKAALGR 85 
                                               7789********************************************99******************* PP

                                 TIGR01141  67 ylgveeenillgnGsdelielliraflepgdavlvleptysmYevsakiagaevkevplkedgqedlea 135
                                               +lgv++e+++lgnGs+e++el++r+f++p d+v+++e+++++Y++++++ ga  + +p+++ g +dl+a
  lcl|NCBI__GCF_000423825.1:WP_028990084.1  86 HLGVQPEQVVLGNGSNEILELVTRTFVTPADEVVFSEHAFAVYPIVTQAVGATARVAPARDYG-HDLDA 153
                                               ************************************************************995.9999* PP

                                 TIGR01141 136 vleaakekvklvflasPnnPtGnllkreeiekvleev.edalVVvDeAYieFsee...asvlellaeyp 200
                                               ++  ++e+++lvf+a+PnnPtG++l re +e+++ ++ +++lVV+DeAY eF +    ++ + +l++++
  lcl|NCBI__GCF_000423825.1:WP_028990084.1 154 MAGLVNENTRLVFVANPNNPTGTYLAREALEAFITSMpSHVLVVLDEAYFEFVDApdyPDGIAWLERFD 222
                                               *99**********************************88***************999999********* PP

                                 TIGR01141 201 nlvvlrTlSKafgLAglRvGyaianaeiiealekvrapynvsslaleaavaalrdsdkiektveevkke 269
                                               nl+v+rT+SK++gLAglRvGy+++++++i+ le+vr+p+nv+s a++aa aal d+++++++ + ++++
  lcl|NCBI__GCF_000423825.1:WP_028990084.1 223 NLLVTRTFSKIYGLAGLRVGYGVGHPKLISLLERVRQPFNVNSAAMAAAEAALADHEHLRRSRALNASG 291
                                               ********************************************************************* PP

                                 TIGR01141 270 rerlleelkkleglevyeSkaNFvlikvkedaeelleallekgiivRdlksaeglleeclRitvGtree 338
                                               +++l +++++l gl+  +S +NF+ ++v +da+ ++eall++g+ivR ++ + g+ +e+lR+t+G +ee
  lcl|NCBI__GCF_000423825.1:WP_028990084.1 292 MAQLVAGIEAL-GLHALPSVGNFLAVHVGRDAAPVYEALLRQGVIVRPVAGY-GM-PEFLRVTIGRPEE 357
                                               ***********.8***************************************.96.************* PP

                                 TIGR01141 339 nerllealk 347
                                               n r+l+al+
  lcl|NCBI__GCF_000423825.1:WP_028990084.1 358 NARFLQALE 366
                                               *******97 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (349 nodes)
Target sequences:                          1  (371 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 9.21
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis