Alignments for a candidate for glnA in Azohydromonas australica DSM 1124

GapMind for Amino acid biosynthesis

Alignments for a candidate for glnA in Azohydromonas australica DSM 1124

Align Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I beta; GSI beta; EC 6.3.1.2 (characterized)
to candidate WP_029000178.1 H537_RS0125925 type I glutamate--ammonia ligase

Query= SwissProt::Q3V5W6
         (468 letters)



>NCBI__GCF_000430725.1:WP_029000178.1
          Length = 471

 Score =  640 bits (1651), Expect = 0.0
 Identities = 299/463 (64%), Positives = 376/463 (81%), Gaps = 1/463 (0%)

Query: 5   VQLIKDHDVKWIDLRFTDTKGTQHHVTMPARDALEDDFFEVGKMFDGSSIAGWKGIEASD 64
           ++++K+++VK++D RFTDT+G + HV++P     ++D F+ G  FDGSSIAGWKGIEASD
Sbjct: 9   MKIVKENEVKFVDFRFTDTRGKEQHVSVPVSH-FDEDKFQSGHAFDGSSIAGWKGIEASD 67

Query: 65  MILLPDDDTAVLDPFTEDATLILVCDIIEPSTMQGYDRDPRAIAHRAEEYLKTTGIGDTV 124
           M+L+PD +TA +DPF E+ TLIL CD+I+P+  + Y+RDPR++A RAE Y++ +G+GDT 
Sbjct: 68  MLLMPDPNTANIDPFFEEPTLILTCDVIDPADGKPYERDPRSLAKRAEAYMRASGLGDTA 127

Query: 125 FAGPEPEFFIFDEVKFKSDISGSMFKIYSEQGSWMSDQDIEGGNKGHRPGVKGGYFPVPP 184
           F GPEPEFF+FD V++  D+SG   KI SE+ +W + ++ E GN G RP VKGGYFPVPP
Sbjct: 128 FFGPEPEFFVFDSVRWNIDMSGCFSKIESEEAAWNTGKEYEHGNSGFRPSVKGGYFPVPP 187

Query: 185 FDHDHEIRTAMCNALEEMGQTVEVHHHEVATAGQNEIGVKFNTLVKKADEVQTLKYVVHN 244
            D   ++R+ MC  LE++G  VEVHHHEVATAGQ EIG KF+TL+++AD VQ  KYV+HN
Sbjct: 188 VDSGQDLRSEMCLLLEQLGIPVEVHHHEVATAGQMEIGTKFSTLIERADWVQLQKYVIHN 247

Query: 245 VADAYGRTATFMPKPLYGDNGSGMHVHMSIAKDGKNTFAGEGYAGLSETALYFIGGIIKH 304
           VA AYG+TATFMPKP+ GDNGSGMHVH S+ KDGKN FAG+GYAGLS+ ALY+IGGIIKH
Sbjct: 248 VAAAYGKTATFMPKPIVGDNGSGMHVHQSVWKDGKNLFAGDGYAGLSDFALYYIGGIIKH 307

Query: 305 GKALNGFTNPATNSYKRLVPGFEAPVMLAYSARNRSASIRIPYVNSPRGRRIEARFPDPA 364
            +ALN  TNP TNSYKRLVPGFEAPV LAYSA+NRSASIRIPYV +P+GRR+EARFPDP 
Sbjct: 308 ARALNAITNPGTNSYKRLVPGFEAPVKLAYSAKNRSASIRIPYVANPKGRRVEARFPDPL 367

Query: 365 ANPYLAFAALLMAGLDGIQNKIHPGDAADKNLYDLPPEEAKEIPQVCGSLKEALEELDKG 424
            NPYL FAALLMAGLDG++NKIHPG+AA K+LY LPPEE  +IP VC SL +ALE LDK 
Sbjct: 368 MNPYLGFAALLMAGLDGVENKIHPGEAATKDLYHLPPEEDAKIPTVCHSLDQALEALDKD 427

Query: 425 RAFLTKGGVFSDDFIDAYIALKSEEEIKVRTFVHPLEYELYYS 467
           RAFLTKGGVF+D +IDAYI LK +E  + R   HP+E+++YY+
Sbjct: 428 RAFLTKGGVFTDSYIDAYIDLKMQEVTRFRMATHPVEFDMYYA 470


Lambda     K      H
   0.318    0.137    0.409 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 762
Number of extensions: 36
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 468
Length of database: 471
Length adjustment: 33
Effective length of query: 435
Effective length of database: 438
Effective search space:   190530
Effective search space used:   190530
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 51 (24.3 bits)

Align candidate WP_029000178.1 H537_RS0125925 (type I glutamate--ammonia ligase)
to HMM TIGR00653 (glnA: glutamine synthetase, type I (EC 6.3.1.2))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00653.hmm
# target sequence database:        /tmp/gapView.9190.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00653  [M=462]
Accession:   TIGR00653
Description: GlnA: glutamine synthetase, type I
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   4.1e-206  671.0   0.0   4.6e-206  670.9   0.0    1.0  1  lcl|NCBI__GCF_000430725.1:WP_029000178.1  H537_RS0125925 type I glutamate-


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000430725.1:WP_029000178.1  H537_RS0125925 type I glutamate--ammonia ligase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  670.9   0.0  4.6e-206  4.6e-206       2     461 ..       7     469 ..       6     470 .. 0.99

  Alignments for each domain:
  == domain 1  score: 670.9 bits;  conditional E-value: 4.6e-206
                                 TIGR00653   2 evlkllkeenvkfvdlrfvDikGklkkveipvseleeealeegiaFDgssveGfksieesDlllkpdpe 70 
                                               +v+k++ke++vkfvd+rf+D++Gk+++v++pvs+++e+ +++g aFDgss+ G+k+ie sD+ll+pdp+
  lcl|NCBI__GCF_000430725.1:WP_029000178.1   7 DVMKIVKENEVKFVDFRFTDTRGKEQHVSVPVSHFDEDKFQSGHAFDGSSIAGWKGIEASDMLLMPDPN 75 
                                               6899***************************************************************** PP

                                 TIGR00653  71 tlvivPfraekvlrvicdvyepvtkepyerdpRsiakraeeelk.tklgdevyfGpEaEFflfdkvefk 138
                                               t+ i+Pf +e++l+++cdv +p++++pyerdpRs akrae +++ ++lgd+++fGpE+EFf+fd+v+ +
  lcl|NCBI__GCF_000430725.1:WP_029000178.1  76 TANIDPFFEEPTLILTCDVIDPADGKPYERDPRSLAKRAEAYMRaSGLGDTAFFGPEPEFFVFDSVRWN 144
                                               ********************************************9************************ PP

                                 TIGR00653 139 easnssflevdseegewn..reveegnkgykikkkggYfdvepvDkakdirrelvlaleelglevevsH 205
                                                 ++  f +++see++wn  +e+e+gn+g++++ kggYf+v+pvD+ +d+r+e++l le+lg+ vev+H
  lcl|NCBI__GCF_000430725.1:WP_029000178.1 145 IDMSGCFSKIESEEAAWNtgKEYEHGNSGFRPSVKGGYFPVPPVDSGQDLRSEMCLLLEQLGIPVEVHH 213
                                               ******************999************************************************ PP

                                 TIGR00653 206 HEvata.qaEidikfdklvkaaDeivlyKyvvknvakkhGktatFmpKplfgdngsGmHvhlslwkdge 273
                                               HEvata q Ei++kf++l++ aD ++l Kyv++nva+ +GktatFmpKp++gdngsGmHvh+s+wkdg+
  lcl|NCBI__GCF_000430725.1:WP_029000178.1 214 HEVATAgQMEIGTKFSTLIERADWVQLQKYVIHNVAAAYGKTATFMPKPIVGDNGSGMHVHQSVWKDGK 282
                                               ********************************************************************* PP

                                 TIGR00653 274 nlfageegyagLsetalyyigGilkHakalaAltnptvnsYkRLvpGyEAPvylaysaknRsaliRiPa 342
                                               nlfag+  yagLs+ alyyigGi+kHa+al+A+tnp +nsYkRLvpG+EAPv+laysaknRsa+iRiP+
  lcl|NCBI__GCF_000430725.1:WP_029000178.1 283 NLFAGDG-YAGLSDFALYYIGGIIKHARALNAITNPGTNSYKRLVPGFEAPVKLAYSAKNRSASIRIPY 350
                                               ******9.************************************************************* PP

                                 TIGR00653 343 sanpkakRiEvRspDpsanpYLafaallmAgldGiknkidpgepldknlyelseeelkelGieqlpesL 411
                                               +anpk++R+E R+pDp  npYL faallmAgldG++nki+pge+  k+ly+l++ee ++  i+++ +sL
  lcl|NCBI__GCF_000430725.1:WP_029000178.1 351 VANPKGRRVEARFPDPLMNPYLGFAALLMAGLDGVENKIHPGEAATKDLYHLPPEEDAK--IPTVCHSL 417
                                               ***********************************************************..******** PP

                                 TIGR00653 412 eealdelesdk..evlkevlgeelieafielkrkEveelrlkvhpvElekyl 461
                                               ++al++l++d+  +++++v+++  i+a+i+lk++Ev ++r++ hpvE+ +y+
  lcl|NCBI__GCF_000430725.1:WP_029000178.1 418 DQALEALDKDRafLTKGGVFTDSYIDAYIDLKMQEVTRFRMATHPVEFDMYY 469
                                               ***********9999************************************9 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (462 nodes)
Target sequences:                          1  (471 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.02
# Mc/sec: 10.82
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis