Align LL-diaminopimelate aminotransferase (EC 2.6.1.83) (characterized)
to candidate WP_029133026.1 A3GO_RS0108435 LL-diaminopimelate aminotransferase
Query= BRENDA::O26158 (410 letters) >NCBI__GCF_000428045.1:WP_029133026.1 Length = 408 Score = 537 bits (1384), Expect = e-157 Identities = 259/409 (63%), Positives = 318/409 (77%), Gaps = 3/409 (0%) Query: 1 MVTVNENYLLLKSSYIFSEINRRVEEFQRKNPDADIIRMGIGDVTRPLPEAVVEAFHRAV 60 M+ +NENY L++SY+FS I +RV FQ+ N D +I++GIGDVTR LP+A V+AFH AV Sbjct: 1 MIKINENYQKLQASYLFSNIAKRVNAFQQSNQDKKLIKLGIGDVTRALPDACVKAFHLAV 60 Query: 61 DEMAEEETFRGYGPEQGYPFLREAIAENDYASRGVDITADEIFISDGAKCDTGNIQEIFG 120 DEMA + TF GYGPEQGY FLREAIA+ND+ +RG + ADE+F+SDGAKCD+GN QEIF Sbjct: 61 DEMANDATFHGYGPEQGYDFLREAIAKNDFQARGAQVDADEVFVSDGAKCDSGNFQEIFS 120 Query: 121 LDNVVAVTDPVYPVYVESNVMAGRAGPADDD-GRYSGLVYLPCTEENSFIPSLPEERVDL 179 D VA+ DPVYPVYV++NVMAGR+G D + GRY LVYL +N FIP LP+ VDL Sbjct: 121 TDITVAIPDPVYPVYVDTNVMAGRSGSWDAELGRYENLVYLDGNRDNGFIPDLPDRPVDL 180 Query: 180 IYLCYPNNPTGTTLTEKQLAEWVDYARDSGSLILFDAAYEAYIQEDGIPHSIYEVEGARE 239 IYLC+PNNP G+T T +QL +WVDYA+ + SLILFDAAYEA++Q+ +P SIYEVEGARE Sbjct: 181 IYLCFPNNPAGSTATREQLKQWVDYAKANKSLILFDAAYEAFVQDADLPRSIYEVEGARE 240 Query: 240 VAIEFRSFSKNAGFTGTRCAFTVVPEELEVPDSSGRMHSVRELWNRRQTTKFNGVSYPVQ 299 VA+EFRSFSK AGFTGTRCA+TVVP+E +G+ SV LWNRR TTKFN VSYPVQ Sbjct: 241 VAVEFRSFSKTAGFTGTRCAYTVVPKECMAYTEAGQAVSVHSLWNRRHTTKFNSVSYPVQ 300 Query: 300 RAAEAVYTPEGQREIRESIDYYMENARIIRESLERAGLRYYGGVNAPYIWIRTPEGMDSW 359 RAAEAVY+ EGQ +I+E I YY+ NA+ IRE +E G GGVN+PYIWI + DSW Sbjct: 301 RAAEAVYSAEGQAQIKELIAYYLNNAKYIREKMEALGYECIGGVNSPYIWIDSK--CDSW 358 Query: 360 QFFDTLLNDAEVVGTPGSGFGPSGEGYFRLTAFNSFRNTVKAMERISEL 408 +FFD LLN+A VV TPG+GFG GEGY R++AFNS+ N +AMERIS++ Sbjct: 359 EFFDRLLNEAAVVCTPGAGFGKCGEGYIRISAFNSYENVTEAMERISKI 407 Lambda K H 0.317 0.136 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 569 Number of extensions: 21 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 410 Length of database: 408 Length adjustment: 31 Effective length of query: 379 Effective length of database: 377 Effective search space: 142883 Effective search space used: 142883 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 50 (23.9 bits)
Align candidate WP_029133026.1 A3GO_RS0108435 (LL-diaminopimelate aminotransferase)
to HMM TIGR03542 (LL-diaminopimelate aminotransferase (EC 2.6.1.83))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR03542.hmm # target sequence database: /tmp/gapView.18663.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR03542 [M=402] Accession: TIGR03542 Description: DAPAT_plant: LL-diaminopimelate aminotransferase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.8e-180 586.0 0.4 2.1e-180 585.8 0.4 1.0 1 lcl|NCBI__GCF_000428045.1:WP_029133026.1 A3GO_RS0108435 LL-diaminopimelat Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000428045.1:WP_029133026.1 A3GO_RS0108435 LL-diaminopimelate aminotransferase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 585.8 0.4 2.1e-180 2.1e-180 2 400 .. 3 406 .. 2 408 .] 0.98 Alignments for each domain: == domain 1 score: 585.8 bits; conditional E-value: 2.1e-180 TIGR03542 2 kvnehflklkssylfseiakkvkefrkknpeadiirlgiGdvtqPlakaviealkeaveelaseetfrG 70 k+ne+++kl++sylfs+iak+v+ f+++n ++++i+lgiGdvt+ l+ a+++a++ av+e+a++ tf G lcl|NCBI__GCF_000428045.1:WP_029133026.1 3 KINENYQKLQASYLFSNIAKRVNAFQQSNQDKKLIKLGIGDVTRALPDACVKAFHLAVDEMANDATFHG 71 89******************************************************************* PP TIGR03542 71 yGpeqGldllreaiaeeeykgr...idpeeifisdGakcdvarllelfgsdktvavqdPvyPvyvdsav 136 yGpeqG+d+lreaia++++++r +d++e+f+sdGakcd ++++e+f++d tva+ dPvyPvyvd++v lcl|NCBI__GCF_000428045.1:WP_029133026.1 72 YGPEQGYDFLREAIAKNDFQARgaqVDADEVFVSDGAKCDSGNFQEIFSTDITVAIPDPVYPVYVDTNV 140 *********************9999******************************************** PP TIGR03542 137 laGragvld.ddgkykkivylpctkenefiPelpeekkvdiiylCsPnnPtGtvltkeqlkelvdyake 204 +aGr+g d + g+y+++vyl ++n+fiP+lp+++ vd+iylC+PnnP+G+++t+eqlk++vdyak+ lcl|NCBI__GCF_000428045.1:WP_029133026.1 141 MAGRSGSWDaELGRYENLVYLDGNRDNGFIPDLPDRP-VDLIYLCFPNNPAGSTATREQLKQWVDYAKA 208 *********899************************9.******************************* PP TIGR03542 205 ndslilfdaayeafisdpslPksiyeiegakecaieirsfsktlGftGvrlgytvvPkelkvedg.... 269 n+slilfdaayeaf++d +lP+siye+ega+e+a+e+rsfskt+GftG+r++ytvvPke ++ ++ lcl|NCBI__GCF_000428045.1:WP_029133026.1 209 NKSLILFDAAYEAFVQDADLPRSIYEVEGAREVAVEFRSFSKTAGFTGTRCAYTVVPKECMAYTEagqa 277 ***********************************************************9876666777 PP TIGR03542 270 eslkklWerllctkfnGaslivqraaeavlakeglqqikeaisyymenarilrkaleaaglkvyGGena 338 s+++lW+r+ +tkfn +s++vqraaeav++ eg+ qike+i+yy++na+ +r+++ea g++ GG+n+ lcl|NCBI__GCF_000428045.1:WP_029133026.1 278 VSVHSLWNRRHTTKFNSVSYPVQRAAEAVYSAEGQAQIKELIAYYLNNAKYIREKMEALGYECIGGVNS 346 99******************************************************************* PP TIGR03542 339 PylWvklpegissedffdkllkeaevvgtPGsGfGssGeGfvrlsalgkredivealerike 400 Py+W+ ++ +s++ffd+ll+ea vv tPG GfG++GeG++r+sa++++e+++ea+eri++ lcl|NCBI__GCF_000428045.1:WP_029133026.1 347 PYIWID--SKCDSWEFFDRLLNEAAVVCTPGAGFGKCGEGYIRISAFNSYENVTEAMERISK 406 ****86..567*************************************************97 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (402 nodes) Target sequences: 1 (408 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.01 # Mc/sec: 10.70 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory