Alignments for a candidate for leuB in Thiomicrospira pelophila DSM 1534

GapMind for Amino acid biosynthesis

Alignments for a candidate for leuB in Thiomicrospira pelophila DSM 1534

Align 3-isopropylmalate dehydrogenase; 3-IPM-DH; Beta-IPM dehydrogenase; IMDH; EC 1.1.1.85 (characterized)
to candidate WP_029933935.1 N746_RS0103275 3-isopropylmalate dehydrogenase

Query= SwissProt::Q56268
         (358 letters)



>NCBI__GCF_000711195.1:WP_029933935.1
          Length = 358

 Score =  436 bits (1122), Expect = e-127
 Identities = 224/355 (63%), Positives = 274/355 (77%), Gaps = 2/355 (0%)

Query: 2   KKIAIFAGDGIGPEIVAAARQVLDAVDQAAHLGLRCTEGLVGGAALDASDDPLPAASLQL 61
           KK+ +  GDGIGPEIVA A +VLDA+     L +    GL+GGAA DAS  PL   ++Q+
Sbjct: 3   KKVLLLPGDGIGPEIVAEAVKVLDALKANHGLDIEMVSGLIGGAAYDASGHPLTDETMQM 62

Query: 62  AMAADAVILGAVGGPRWDAYPPAKRPEQGLLRLRKGLDLYANLRPAQIFPQLLDASPLRP 121
           A   DA++LGAVGG +W++   + RPE+GLL LR GL L+ANLRPA ++PQL DAS L+P
Sbjct: 63  ARDVDAILLGAVGGYKWESLDISVRPEKGLLGLRSGLSLFANLRPAFLYPQLADASTLKP 122

Query: 122 ELVRDVDILVVRELTGDIYFGQPRGLEVID-GKRRGFNTMVYDEDEIRRIAHVAFRAAQG 180
           E+V  +DIL+VRELTG IYFGQPRG+  ++ G+R+G+NT VY E EI RI HVAF+AA  
Sbjct: 123 EVVSGLDILIVRELTGGIYFGQPRGIRTLENGERQGYNTYVYSESEIVRIGHVAFQAAMK 182

Query: 181 RRKQLCSVDKANVLETTRLWREVVTEVARDYPDVRLSHMYVDNAAMQLIRAPAQFDVLLT 240
           R K++CSVDKANVLE T LWREV+T++A+ YP+V L+HMYVDNAAMQL+R P QFDV++T
Sbjct: 183 RNKKVCSVDKANVLEVTELWREVMTDLAKQYPEVELTHMYVDNAAMQLVRNPKQFDVVVT 242

Query: 241 GNMFGDILSDEASQLTGSIGMLPSASL-GEGRAMYEPIHGSAPDIAGQDKANPLATILSV 299
           GNMFGDILSDEAS LTGSIGMLPSASL    + MYEP HGSAPDIAGQ+ ANPLATILS 
Sbjct: 243 GNMFGDILSDEASMLTGSIGMLPSASLDANNKGMYEPSHGSAPDIAGQNLANPLATILSA 302

Query: 300 AMMLRHSLNAEPWAQRVEAAVQRVLDQGLRTADIAAPGTPVIGTKAMGAAVVNAL 354
           AMMLR+SL  E  A ++E AV +VLDQGLRT DI + G   + T  MG+AVV AL
Sbjct: 303 AMMLRYSLGREDLALKIEQAVSKVLDQGLRTGDIYSEGMKKVSTSEMGSAVVKAL 357


Lambda     K      H
   0.320    0.137    0.396 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 424
Number of extensions: 10
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 358
Length of database: 358
Length adjustment: 29
Effective length of query: 329
Effective length of database: 329
Effective search space:   108241
Effective search space used:   108241
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 49 (23.5 bits)

Align candidate WP_029933935.1 N746_RS0103275 (3-isopropylmalate dehydrogenase)
to HMM TIGR00169 (leuB: 3-isopropylmalate dehydrogenase (EC 1.1.1.85))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00169.hmm
# target sequence database:        /tmp/gapView.21843.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00169  [M=349]
Accession:   TIGR00169
Description: leuB: 3-isopropylmalate dehydrogenase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   1.1e-157  510.5   0.1   1.3e-157  510.3   0.1    1.0  1  lcl|NCBI__GCF_000711195.1:WP_029933935.1  N746_RS0103275 3-isopropylmalate


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000711195.1:WP_029933935.1  N746_RS0103275 3-isopropylmalate dehydrogenase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  510.3   0.1  1.3e-157  1.3e-157       1     347 [.       4     351 ..       4     353 .. 0.99

  Alignments for each domain:
  == domain 1  score: 510.3 bits;  conditional E-value: 1.3e-157
                                 TIGR00169   1 kiavLpGDgiGpevvaealkvLkaveerfelklefeealiGGaaidatgePlpeetlkackeadavLlg 69 
                                               k+ +LpGDgiGpe+vaea+kvL+a++ +++l++e+ + liGGaa da g+Pl++et++ ++++da+Llg
  lcl|NCBI__GCF_000711195.1:WP_029933935.1   4 KVLLLPGDGIGPEIVAEAVKVLDALKANHGLDIEMVSGLIGGAAYDASGHPLTDETMQMARDVDAILLG 72 
                                               6899***************************************************************** PP

                                 TIGR00169  70 avGGpkWdnlprdvrPekgLLklrkeldlfanLrPaklfksLeklsplkeeivkgvDlvvvreLtgGiY 138
                                               avGG+kW++l  +vrPekgLL lr  l lfanLrPa l+++L ++s+lk+e+v+g+D+++vreLtgGiY
  lcl|NCBI__GCF_000711195.1:WP_029933935.1  73 AVGGYKWESLDISVRPEKGLLGLRSGLSLFANLRPAFLYPQLADASTLKPEVVSGLDILIVRELTGGIY 141
                                               ********************************************************************* PP

                                 TIGR00169 139 fGepkereeaee.ekkaldtekYtkeeieriarvafelarkrrkkvtsvDkanvLessrlWrktveeia 206
                                               fG+p++++  e+ e+++++t +Y+++ei ri +vaf++a+kr+kkv+svDkanvLe+++lWr++++++a
  lcl|NCBI__GCF_000711195.1:WP_029933935.1 142 FGQPRGIRTLENgERQGYNTYVYSESEIVRIGHVAFQAAMKRNKKVCSVDKANVLEVTELWREVMTDLA 210
                                               ********99999******************************************************** PP

                                 TIGR00169 207 keyPdvelehlyiDnaamqLvksPeqldvvvtsnlfGDilsDeasvitGslGlLPsaslsskglalfep 275
                                               k+yP+vel h+y+DnaamqLv++P+q+dvvvt+n+fGDilsDeas++tGs+G+LPsasl  + ++++ep
  lcl|NCBI__GCF_000711195.1:WP_029933935.1 211 KQYPEVELTHMYVDNAAMQLVRNPKQFDVVVTGNMFGDILSDEASMLTGSIGMLPSASLDANNKGMYEP 279
                                               ********************************************************************* PP

                                 TIGR00169 276 vhgsapdiagkgianpiaailsaalllryslnleeaaeaieaavkkvleegkrtedlaseattavstke 344
                                                hgsapdiag+++anp+a+ilsaa++lrysl+ e+ a +ie+av+kvl++g rt d++se+ ++vst+e
  lcl|NCBI__GCF_000711195.1:WP_029933935.1 280 SHGSAPDIAGQNLANPLATILSAAMMLRYSLGREDLALKIEQAVSKVLDQGLRTGDIYSEGMKKVSTSE 348
                                               ********************************************************************* PP

                                 TIGR00169 345 vee 347
                                               +++
  lcl|NCBI__GCF_000711195.1:WP_029933935.1 349 MGS 351
                                               986 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (349 nodes)
Target sequences:                          1  (358 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.01
# Mc/sec: 6.75
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis