Alignments for a candidate for cimA in Thiomicrospira pelophila DSM 1534

GapMind for Amino acid biosynthesis

Alignments for a candidate for cimA in Thiomicrospira pelophila DSM 1534

Align Putative (R)-citramalate synthase CimA; EC 2.3.3.21 (uncharacterized)
to candidate WP_029935937.1 N746_RS0109030 2-isopropylmalate synthase

Query= curated2:Q8TYM1
         (509 letters)



>NCBI__GCF_000711195.1:WP_029935937.1
          Length = 516

 Score =  362 bits (928), Expect = e-104
 Identities = 209/502 (41%), Positives = 303/502 (60%), Gaps = 17/502 (3%)

Query: 12  DEVRIFDTTLRDGEQTPGVALTPEEKLRIARKLDEIGVDTIEAGFAAASEGELKAIRRIA 71
           D + IFDTTLRDGEQ+PG ++T EEK+RIA++L+++ VD IEAGF AAS+G+  +++ +A
Sbjct: 3   DHLIIFDTTLRDGEQSPGASMTKEEKVRIAKQLEKLRVDVIEAGFPAASQGDFDSVKAVA 62

Query: 72  REELDAEVCSMARMVKGDVDAAVE----AEADAVHIVVPTSEVHVKKKLRMDREEVLERA 127
               D+ +C +AR V+ D+  A E    A +  +H  + TS +H++ KLRM  +EV+ERA
Sbjct: 63  SAIKDSTICGLARAVENDIARAGEAIKPANSGRIHTFIATSPIHMQMKLRMSPDEVVERA 122

Query: 128 REVVEYARDHGLTVEISTEDGTRTELEYLYEVFDACLEAGAERLGYNDTVGVMAPEGMFL 187
              V+ AR +   VE S ED  R++L++L  V +A ++AGA  +   DTVG   PE    
Sbjct: 123 VWAVKQARRYTDDVEFSPEDAGRSDLDFLCRVVEAVIKAGATTINMPDTVGYNVPEQFGA 182

Query: 188 AVKKLRERV--GEDVILSVHCHDDFGMATANTVAAVRAGARQVHVTVNGIGERAGNAALE 245
             K + ERV   +  + S HCH+D G+A AN++AAV  GARQV  T+NG+GERAGN ALE
Sbjct: 183 LFKNVMERVPNSDKAVFSAHCHNDLGLAVANSLAAVSNGARQVECTINGLGERAGNTALE 242

Query: 246 EVVVVL---EELYGVDTGIRTERLTELSKLVERLTGVRVPPNKAVVGENAFTHESGIHAD 302
           EVV+ +   ++ + VDT I T  +   S+LV  +TG  V PNKA+VG NAF HESGIH D
Sbjct: 243 EVVMAVRTRQDHFSVDTRIHTPEILNASRLVSNITGFNVQPNKAIVGANAFAHESGIHQD 302

Query: 303 GILKDESTYEPIPPEKVGH-ERRFVLGKHVGTSVIRKKLKQMGVDVD-DEQLLEILRRLK 360
           G+LK   TYE +  E VG    + VLGKH G +  R +L ++G++ + +EQL +   R K
Sbjct: 303 GVLKHRETYEIMRAEDVGWVTNKMVLGKHSGRNAFRTRLLELGIEFETEEQLNDAFLRFK 362

Query: 361 RLGDRGKRITEADLRAIAEDVLGRPAERD-IEVEDFTTVTGKRTIPTASIVVKIDGTRKE 419
            L DR   I + DL+A+  D      E + I +      T     P A++ + ++G    
Sbjct: 363 ELADRKHEIYDEDLQALVTDANNERLENESIRLMALRVSTEMGEAPHATLTLWVNGVEHT 422

Query: 420 AASTGVGPVDATIKALERALKDQGIDFELVEYRAEALTGGTDAITHVDVKLRDPETGDIV 479
           A + G G VDAT KA+E+ +   G   EL  Y    +T GTD++    V+L   + G I+
Sbjct: 423 ANAKGGGVVDATFKAIEQII-HSGTTLEL--YSVSNVTNGTDSLGETTVRLE--KGGRIL 477

Query: 480 HSGSSREDIVVASLEAFIDGIN 501
           +   S  DIV AS +A+I+ +N
Sbjct: 478 NGQGSDTDIVTASAKAYINALN 499


Lambda     K      H
   0.315    0.134    0.367 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 617
Number of extensions: 38
Number of successful extensions: 8
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 509
Length of database: 516
Length adjustment: 35
Effective length of query: 474
Effective length of database: 481
Effective search space:   227994
Effective search space used:   227994
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis