Alignments for a candidate for serC in Beijerinckia mobilis UQM 1969

GapMind for Amino acid biosynthesis

Alignments for a candidate for serC in Beijerinckia mobilis UQM 1969

Align Phosphoserine aminotransferase; Phosphohydroxythreonine aminotransferase; PSAT; EC 2.6.1.52 (characterized)
to candidate WP_034995218.1 DL88_RS09605 phosphoserine transaminase

Query= SwissProt::P52878
         (370 letters)



>NCBI__GCF_000745425.1:WP_034995218.1
          Length = 390

 Score =  477 bits (1228), Expect = e-139
 Identities = 234/378 (61%), Positives = 272/378 (71%), Gaps = 10/378 (2%)

Query: 3   PTRVPNNPCFSSGPCAKHPGYSIEELKDTPFGRSHRSNLGKEKLAEAIKKTRDMLGLPDD 62
           P   P N CFSSGPCAK PG+    LKD P GRSHR+ +GK KL  AI  TR++L +P D
Sbjct: 6   PAARPANACFSSGPCAKRPGWDFSALKDAPLGRSHRAKIGKTKLKLAIDLTREILQVPAD 65

Query: 63  YLVGIVPASDTGAFEMCLWSMLGCRGVDVLVWESFSKGWATDITKQLKLKDVRVFEAEYG 122
           Y +GIVP SDTGA EM LW++LG RGVDVL WESF +GW TDI KQLKL +VR  +A YG
Sbjct: 66  YRIGIVPGSDTGAVEMALWTLLGARGVDVLAWESFGQGWVTDIVKQLKLPNVRTLKAGYG 125

Query: 123 KLPDLKKVDFKNDVVFVWNGTTSGVKVPNGDWIPENREGLTLCDATSAIFAMDIPYHKLD 182
           ++ DL  VDF NDVVF WNGTTSGVKVPNGDWI  +R+GLT+CDATSA FA  + + KLD
Sbjct: 126 EIVDLATVDFNNDVVFTWNGTTSGVKVPNGDWIAADRQGLTICDATSAAFAQKLDWAKLD 185

Query: 183 VITFSWQKVLGGEGAHGMLILSPRAVQRLESYTPAWPLPKIFRLTKGGKLNKKIFEGSTI 242
           V+TFSWQKVLGGEG HGMLILSPRAV+RL +YTPAWPLPKIFRLT GGKL + IF G TI
Sbjct: 186 VVTFSWQKVLGGEGGHGMLILSPRAVERLTTYTPAWPLPKIFRLTSGGKLTEGIFVGETI 245

Query: 243 NTPSMLANEDWLATLKWAESVGGLKPLIQRTNDNLAVFEAFVAKNNWIHFLAETKEIRSS 302
           NTPSML  ED++  L WA+S+GGL  LI R + N      +V K  WI FLA    IRS+
Sbjct: 246 NTPSMLCVEDYIDALNWAKSLGGLDSLIARADANTKAITDWVEKTAWIDFLAADPAIRSN 305

Query: 303 TSVCFKV----------DLSDEKLKELIKTLEKEKVAYDIGSYRDAPSGLRIWCGATVEK 352
           TSVC KV          +   +  K+L   LEKE VAYDIG+YRDAP GLRIWCGATVE 
Sbjct: 306 TSVCLKVSDPEITALPAEAQADFAKKLASLLEKENVAYDIGAYRDAPPGLRIWCGATVET 365

Query: 353 EDLQCLCEWIEWAYNLVK 370
            D+  L  W+++AY   K
Sbjct: 366 SDVTALTAWLDYAYAKTK 383


Lambda     K      H
   0.318    0.136    0.429 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 513
Number of extensions: 19
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 370
Length of database: 390
Length adjustment: 30
Effective length of query: 340
Effective length of database: 360
Effective search space:   122400
Effective search space used:   122400
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 50 (23.9 bits)

Align candidate WP_034995218.1 DL88_RS09605 (phosphoserine transaminase)
to HMM TIGR01365 (phosphoserine aminotransferase (EC 2.6.1.52))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01365.hmm
# target sequence database:        /tmp/gapView.15840.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01365  [M=374]
Accession:   TIGR01365
Description: serC_2: phosphoserine aminotransferase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   5.5e-216  702.8   2.8   6.4e-216  702.5   2.8    1.0  1  lcl|NCBI__GCF_000745425.1:WP_034995218.1  DL88_RS09605 phosphoserine trans


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000745425.1:WP_034995218.1  DL88_RS09605 phosphoserine transaminase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  702.5   2.8  6.4e-216  6.4e-216       1     373 [.       9     381 ..       9     382 .. 1.00

  Alignments for each domain:
  == domain 1  score: 702.5 bits;  conditional E-value: 6.4e-216
                                 TIGR01365   1 rpanpefssgpcakrpgysveelknaalgrshrsklgkeklkeaiektrevlevpadyligivaasdtg 69 
                                               rpan +fssgpcakrpg+   +lk+a+lgrshr+k+gk+klk ai+ tre+l+vpady+igiv++sdtg
  lcl|NCBI__GCF_000745425.1:WP_034995218.1   9 RPANACFSSGPCAKRPGWDFSALKDAPLGRSHRAKIGKTKLKLAIDLTREILQVPADYRIGIVPGSDTG 77 
                                               7******************************************************************** PP

                                 TIGR01365  70 avemalwsllgargvdllafesfgkgwvtdvtkqlklkdvrvleaeygklpdlkkvdfkkdvvftwngt 138
                                               avemalw+llgargvd+la+esfg+gwvtd++kqlkl++vr l+a+yg++ dl+ vdf++dvvftwngt
  lcl|NCBI__GCF_000745425.1:WP_034995218.1  78 AVEMALWTLLGARGVDVLAWESFGQGWVTDIVKQLKLPNVRTLKAGYGEIVDLATVDFNNDVVFTWNGT 146
                                               ********************************************************************* PP

                                 TIGR01365 139 tsgvrvpngdfipadreglticdatsaafaqdldyekldvvtfswqkvlggegahgvlilspravarle 207
                                               tsgv+vpngd+i+adr+glticdatsaafaq+ld+ kldvvtfswqkvlggeg hg+lilsprav+rl 
  lcl|NCBI__GCF_000745425.1:WP_034995218.1 147 TSGVKVPNGDWIAADRQGLTICDATSAAFAQKLDWAKLDVVTFSWQKVLGGEGGHGMLILSPRAVERLT 215
                                               ********************************************************************* PP

                                 TIGR01365 208 sytpawplpkifrltkggklskdifegetintpsmlavedaldalkwaesigglkalvaraddnlavle 276
                                               +ytpawplpkifrlt+ggkl+++if getintpsml+ved++dal+wa+s+ggl++l+arad+n + + 
  lcl|NCBI__GCF_000745425.1:WP_034995218.1 216 TYTPAWPLPKIFRLTSGGKLTEGIFVGETINTPSMLCVEDYIDALNWAKSLGGLDSLIARADANTKAIT 284
                                               ********************************************************************* PP

                                 TIGR01365 277 afvaksswvdflaatkeirsntsvclkvvdpdvaaldedaqadfakelvsalekegvaydigsyrdapa 345
                                               ++v+k++w+dflaa ++irsntsvclkv dp+++al+++aqadfak+l+s+leke+vaydig+yrdap+
  lcl|NCBI__GCF_000745425.1:WP_034995218.1 285 DWVEKTAWIDFLAADPAIRSNTSVCLKVSDPEITALPAEAQADFAKKLASLLEKENVAYDIGAYRDAPP 353
                                               ********************************************************************* PP

                                 TIGR01365 346 glriwcgatveksdleallewldwafal 373
                                               glriwcgatve+sd+ al+ wld+a+a 
  lcl|NCBI__GCF_000745425.1:WP_034995218.1 354 GLRIWCGATVETSDVTALTAWLDYAYAK 381
                                               **************************85 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (374 nodes)
Target sequences:                          1  (390 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 8.38
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis