Alignments for a candidate for dapB in Desulfobacter vibrioformis DSM 8776

GapMind for Amino acid biosynthesis

Alignments for a candidate for dapB in Desulfobacter vibrioformis DSM 8776

Align 4-hydroxy-tetrahydrodipicolinate reductase (EC 1.17.1.8) (characterized)
to candidate WP_035236887.1 Q366_RS04940 dihydrodipicolinate reductase

Query= BRENDA::D8R6G2
         (288 letters)



>NCBI__GCF_000745975.1:WP_035236887.1
          Length = 278

 Score =  250 bits (639), Expect = 2e-71
 Identities = 127/273 (46%), Positives = 168/273 (61%), Gaps = 2/273 (0%)

Query: 15  PVMVNDCTGKVGQAVAEAAVA-AGLRLVPLSLTGPGRGGKRVVIGNVEVDVREVSEREDV 73
           P+M+N   G V + + EAA       LVP SLTG      +V +   +V + + SERED 
Sbjct: 5   PIMINGLPGNVARIMTEAAFQDERFTLVPFSLTGEEISMTQVSVDQTKVTLLKPSEREDR 64

Query: 74  VKEVITEYPNVIVVDYTLPAAVNDNAEFYCKQGLPFVMGTTGGDREKLLDVARKSGTYSI 133
           +  ++  YP  I VDYT P AVN+NA+FY    +PFVMGTTGGDR+ L +        ++
Sbjct: 65  INNILESYPGCICVDYTHPTAVNNNAKFYVAHKIPFVMGTTGGDRQDLENTVNNGSVPAV 124

Query: 134 IAPQMGKQVVAFVAAMEIMAKQFPGAFSGYTLQVTESHQSTKADVSGTALAVISSLRKLG 193
           IAP M KQ+V   A +E  A  FPG F G+TLQV ESHQ  KAD SGTA A+++   +LG
Sbjct: 125 IAPNMAKQIVGLQAMLEYGASTFPGLFKGFTLQVKESHQQAKADTSGTAKALVACFNQLG 184

Query: 194 LDFKDEQVELVRDPKEQMTRMGVPEQHLNGHAFHTYKIISPDGTVFFEFKHNVCGRSIYA 253
            DF    +E +RDPK Q   +GVPEQ++ GH +HTY + +PDG+  FE  HN+ GR IY 
Sbjct: 185 TDFNISDIEKIRDPKIQKADLGVPEQYIEGHGWHTYTLKAPDGSALFELTHNINGRQIYV 244

Query: 254 QGTVDAVLFLSKKIQEKS-EKRLYNMIDVLEGG 285
            GT DAV+FL  KI   +  ++L+ MIDVL  G
Sbjct: 245 SGTFDAVVFLKNKIDANAFGRKLFTMIDVLTAG 277


Lambda     K      H
   0.318    0.134    0.379 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 215
Number of extensions: 10
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 288
Length of database: 278
Length adjustment: 26
Effective length of query: 262
Effective length of database: 252
Effective search space:    66024
Effective search space used:    66024
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 47 (22.7 bits)

Align candidate WP_035236887.1 Q366_RS04940 (dihydrodipicolinate reductase)
to HMM TIGR02130 (dapB: dihydrodipicolinate reductase (EC 1.17.1.8))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR02130.hmm
# target sequence database:        /tmp/gapView.10599.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR02130  [M=275]
Accession:   TIGR02130
Description: dapB_plant: dihydrodipicolinate reductase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   2.2e-107  344.2   0.0   2.4e-107  344.0   0.0    1.0  1  lcl|NCBI__GCF_000745975.1:WP_035236887.1  Q366_RS04940 dihydrodipicolinate


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000745975.1:WP_035236887.1  Q366_RS04940 dihydrodipicolinate reductase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  344.0   0.0  2.4e-107  2.4e-107       1     272 [.       4     277 ..       4     278 .] 0.97

  Alignments for each domain:
  == domain 1  score: 344.0 bits;  conditional E-value: 2.4e-107
                                 TIGR02130   1 vsvmvngisgkmgkivikaav.aaglelvptsltsviiveqevevagkeilllkpserekvlsevleky 68 
                                               + +m+ng++g++ +i+ +aa       lvp+slt+++i   +v v   ++ llkpsere +++++le y
  lcl|NCBI__GCF_000745975.1:WP_035236887.1   4 IPIMINGLPGNVARIMTEAAFqDERFTLVPFSLTGEEISMTQVSVDQTKVTLLKPSEREDRINNILESY 72 
                                               569***************99615689******************************************* PP

                                 TIGR02130  69 pelivvdytipsavndnaelyvkvkvpfvmgttggdrealaklveeakiyaviapqmakqvvaflaaie 137
                                               p  i+vdyt+p+avn+na++yv +k+pfvmgttggdr+ l  +v++  ++aviap+makq+v ++a++e
  lcl|NCBI__GCF_000745975.1:WP_035236887.1  73 PGCICVDYTHPTAVNNNAKFYVAHKIPFVMGTTGGDRQDLENTVNNGSVPAVIAPNMAKQIVGLQAMLE 141
                                               ********************************************************************* PP

                                 TIGR02130 138 ilakefptafegyklevveshqkskldasgtakavvstfqklgvdydmddiekvrdekeqievvgvpee 206
                                               + a  fp+ f+g+ l+v eshq++k+d+sgtaka+v++f++lg d+++ diek+rd+k q   +gvpe+
  lcl|NCBI__GCF_000745975.1:WP_035236887.1 142 YGASTFPGLFKGFTLQVKESHQQAKADTSGTAKALVACFNQLGTDFNISDIEKIRDPKIQKADLGVPEQ 210
                                               ********************************************************************* PP

                                 TIGR02130 207 ylsghafhlysldsadktvsfefqhnvcgrkiyaegtvdavlfladkiiaka.ekkiynmidvlreg 272
                                               y+ gh +h+y+l+++d++  fe+ hn+ gr+iy  gt dav fl +ki a+a  +k + midvl  g
  lcl|NCBI__GCF_000745975.1:WP_035236887.1 211 YIEGHGWHTYTLKAPDGSALFELTHNINGRQIYVSGTFDAVVFLKNKIDANAfGRKLFTMIDVLTAG 277
                                               ************************************************998835789*******988 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (275 nodes)
Target sequences:                          1  (278 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.00
# Mc/sec: 8.14
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis