Alignments for a candidate for argI in Desulfobacter vibrioformis DSM 8776

GapMind for Amino acid biosynthesis

Alignments for a candidate for argI in Desulfobacter vibrioformis DSM 8776

Align ornithine carbamoyltransferase (EC 2.1.3.3) (characterized)
to candidate WP_035242470.1 Q366_RS19245 ornithine carbamoyltransferase

Query= BRENDA::O93656
         (317 letters)



>NCBI__GCF_000745975.1:WP_035242470.1
          Length = 306

 Score =  302 bits (773), Expect = 8e-87
 Identities = 149/305 (48%), Positives = 209/305 (68%), Gaps = 3/305 (0%)

Query: 8   RDLLCLQDYTPEEIWTILETAKMLKIWQKIGKPHRLLEGKTLAMIFQKPSTRTRVSFEVA 67
           +DLLCL D  P++  T+ E A  LK   K G    LL GKTL +IF K STRTR++FE A
Sbjct: 3   KDLLCLLDLEPQDFTTLFERALSLKARHKKGIFDSLLAGKTLGLIFDKKSTRTRIAFETA 62

Query: 68  MAHLGGHALYLNAQDLQLRRGETIADTARVLSRYVDAIMARVYDHKDVEDLAKYASVPVI 127
           M  LGGH++Y++ QD Q+ R E   DTARVLSRY+D +  R +DH  VE  A+ A++PVI
Sbjct: 63  MIQLGGHSIYMSTQDTQISRNEPAKDTARVLSRYIDCLAMRTFDHDLVEAFAQSATIPVI 122

Query: 128 NGLSDFSHPCQALADYMTIWEKKGTIKGVKVVYVGDGNNVAHSLMIAGTKLGADVVVATP 187
           N L+D  HPCQ L+D MT+ E KG  + V++ +VGDGNNVA+S + A + LG D++VA P
Sbjct: 123 NALTDAFHPCQILSDIMTVIEHKGGYEDVRIAWVGDGNNVANSWVNAASVLGLDLIVACP 182

Query: 188 EGYEPDEKVIKWAEQNAAESGGSFELLHDPVKAVKDADVIYTDVWASMGQEAEAEERRKI 247
           + +  + ++I  A    A++  +    +DP +A+K ADV+YTDVWASMG+E + E R K 
Sbjct: 183 DNHHINPEIIAAA---GADTKENIVFTNDPKEAIKGADVVYTDVWASMGEEDQLEGRLKA 239

Query: 248 FRPFQVNKDLVKHAKPDYMFMHCLPAHRGEEVTDDVIDSPNSVVWDEAENRLHAQKAVLA 307
           F+ FQVN++L+K AK D + +HCLPAHRGEE+ + V+++ N+  WD+AEN+ H  KA+L 
Sbjct: 240 FKGFQVNEELLKSAKDDCLVLHCLPAHRGEEIAESVLEAQNAAFWDQAENKRHMHKAILE 299

Query: 308 LLLGG 312
            L+ G
Sbjct: 300 RLILG 304


Lambda     K      H
   0.319    0.134    0.400 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 289
Number of extensions: 17
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 317
Length of database: 306
Length adjustment: 27
Effective length of query: 290
Effective length of database: 279
Effective search space:    80910
Effective search space used:    80910
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 48 (23.1 bits)

Align candidate WP_035242470.1 Q366_RS19245 (ornithine carbamoyltransferase)
to HMM TIGR00658 (argF: ornithine carbamoyltransferase (EC 2.1.3.3))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00658.hmm
# target sequence database:        /tmp/gapView.31737.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00658  [M=304]
Accession:   TIGR00658
Description: orni_carb_tr: ornithine carbamoyltransferase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   3.6e-122  393.3   0.0   4.1e-122  393.1   0.0    1.0  1  lcl|NCBI__GCF_000745975.1:WP_035242470.1  Q366_RS19245 ornithine carbamoyl


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000745975.1:WP_035242470.1  Q366_RS19245 ornithine carbamoyltransferase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  393.1   0.0  4.1e-122  4.1e-122       1     302 [.       3     301 ..       3     303 .. 0.98

  Alignments for each domain:
  == domain 1  score: 393.1 bits;  conditional E-value: 4.1e-122
                                 TIGR00658   1 rhllslldlseeelkellelakklkkekkkgkeekklkgktlaliFekrstRtRvsfevaayelGaqvl 69 
                                               ++ll+lldl+++++++l e+a +lk+++kkg  ++ l gktl+liF+k+stRtR++fe+a+++lG++++
  lcl|NCBI__GCF_000745975.1:WP_035242470.1   3 KDLLCLLDLEPQDFTTLFERALSLKARHKKGIFDSLLAGKTLGLIFDKKSTRTRIAFETAMIQLGGHSI 71 
                                               79******************************************************************* PP

                                 TIGR00658  70 ylnkeelqlgrkesikDtarvlsryvdaivvRvykhedveelakyasvPvingLtdlehPcqilaDllt 138
                                               y++++++q++r+e+ kDtarvlsry+d++++R+++h+ ve++a+ a++Pvin+Ltd +hPcqil+D++t
  lcl|NCBI__GCF_000745975.1:WP_035242470.1  72 YMSTQDTQISRNEPAKDTARVLSRYIDCLAMRTFDHDLVEAFAQSATIPVINALTDAFHPCQILSDIMT 140
                                               ********************************************************************* PP

                                 TIGR00658 139 ikeklgklkevklvyvGDannvanslllaaaklGldvvvatPeglepeaeivkkakkiakenggklelt 207
                                               ++e++g  ++v++++vGD+nnvans + aa  lGld+ va+P++ + ++ei+  a +   ++  ++ +t
  lcl|NCBI__GCF_000745975.1:WP_035242470.1 141 VIEHKGGYEDVRIAWVGDGNNVANSWVNAASVLGLDLIVACPDNHHINPEIIAAAGA---DTKENIVFT 206
                                               *****************************************************9966...66678**** PP

                                 TIGR00658 208 edpkkavkdadviytDvwvsmGeeekkeerlkllkpyqvneellelakpevkflhCLPavrGeevtdev 276
                                               +dpk+a+k+adv+ytDvw+smGee++ e rlk++k++qvneell+ ak++  +lhCLPa+rGee+++ v
  lcl|NCBI__GCF_000745975.1:WP_035242470.1 207 NDPKEAIKGADVVYTDVWASMGEEDQLEGRLKAFKGFQVNEELLKSAKDDCLVLHCLPAHRGEEIAESV 275
                                               ********************************************************************* PP

                                 TIGR00658 277 legeasivfdeaenRlhaqkavlkal 302
                                               le ++   +d+aen+ h  ka+l+ l
  lcl|NCBI__GCF_000745975.1:WP_035242470.1 276 LEAQNAAFWDQAENKRHMHKAILERL 301
                                               **********************9876 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (304 nodes)
Target sequences:                          1  (306 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 6.77
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis