Alignments for a candidate for argC in Phaeacidiphilus oryzae TH49

GapMind for Amino acid biosynthesis

Alignments for a candidate for argC in Phaeacidiphilus oryzae TH49

Align N-acetyl-gamma-glutamyl-phosphate reductase (EC 1.2.1.38) (characterized)
to candidate WP_037570918.1 BS73_RS08940 N-acetyl-gamma-glutamyl-phosphate reductase

Query= BRENDA::P9WPZ9
         (352 letters)



>NCBI__GCF_000744815.1:WP_037570918.1
          Length = 342

 Score =  426 bits (1095), Expect = e-124
 Identities = 216/346 (62%), Positives = 253/346 (73%), Gaps = 7/346 (2%)

Query: 9   ATKVAVAGASGYAGGEILRLLLGHPAYADGRLRIGALTAATSAGSTLGEHHPHLTPLAHR 68
           A + AVAGASGYAGGE+LRLLL HP      + IGA+TA ++AGS LG   PHL PLA R
Sbjct: 2   AYRAAVAGASGYAGGEVLRLLLQHP-----EIEIGAVTAGSNAGSRLGALQPHLAPLADR 56

Query: 69  VVEPTEAAVLGGHDAVFLALPHGHSAVLAQQLSPETLIIDCGADFRLTDAAVWERFYGSS 128
           ++  T A  L GHD VFLALPHG SA +A+QL P+ L++DCGADFRL D A WERFYGS+
Sbjct: 57  ILGETTAETLAGHDVVFLALPHGQSAAVAEQLGPDVLVVDCGADFRLEDPAAWERFYGSA 116

Query: 129 HAGSWPYGLPELPGARDQLRGTRRIAVPGCYPTAALLALFPALAADLIEPAVTVVAVSGT 188
           HAG+WPYGLPELPGAR++L G+RRIAVPGCYPTA  LALFPA AA L EP   VVA SGT
Sbjct: 117 HAGTWPYGLPELPGARERLAGSRRIAVPGCYPTAVSLALFPAYAAGLAEPQAVVVAASGT 176

Query: 189 SGAGRAATTDLLGAEVIGSARAYNIAGVHRHTPEIAQGLRAVTDRDVSVSFTPVLIPASR 248
           SGAG++A   LLG+EV+GS   Y + GVHRHTPE+ Q L AV    V+VSFTP L P  R
Sbjct: 177 SGAGKSAKPHLLGSEVMGSMSPYGVGGVHRHTPEMQQNLSAVAGEPVAVSFTPTLAPMPR 236

Query: 249 GILATCTARTR--SPLSQLRAAYEKAYHAEPFIYLMPEGQLPRTGAVIGSNAAHIAVAVD 306
           GILATC+A+ R  +    LRAAY+K Y  E F+ L+PEGQ P T AV G+N   + VA+D
Sbjct: 237 GILATCSAQARPGTTAESLRAAYQKVYGDEEFVSLLPEGQWPATAAVYGANTVQVQVALD 296

Query: 307 EDAQTFVAIAAIDNLVKGTAGAAVQSMNLALGWPETDGLSVVGVAP 352
           E     + I+AIDNL KGTAG AVQSMNLALG PE  GL  +GVAP
Sbjct: 297 ESVGRIIVISAIDNLTKGTAGGAVQSMNLALGLPEGLGLPKIGVAP 342


Lambda     K      H
   0.318    0.133    0.393 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 467
Number of extensions: 24
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 352
Length of database: 342
Length adjustment: 29
Effective length of query: 323
Effective length of database: 313
Effective search space:   101099
Effective search space used:   101099
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 49 (23.5 bits)

Align candidate WP_037570918.1 BS73_RS08940 (N-acetyl-gamma-glutamyl-phosphate reductase)
to HMM TIGR01850 (argC: N-acetyl-gamma-glutamyl-phosphate reductase (EC 1.2.1.38))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01850.hmm
# target sequence database:        /tmp/gapView.231477.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01850  [M=345]
Accession:   TIGR01850
Description: argC: N-acetyl-gamma-glutamyl-phosphate reductase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                             Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                             -----------
   1.7e-120  388.3   0.0   1.9e-120  388.1   0.0    1.0  1  NCBI__GCF_000744815.1:WP_037570918.1  


Domain annotation for each sequence (and alignments):
>> NCBI__GCF_000744815.1:WP_037570918.1  
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  388.1   0.0  1.9e-120  1.9e-120       2     345 .]       4     342 .]       3     342 .] 0.97

  Alignments for each domain:
  == domain 1  score: 388.1 bits;  conditional E-value: 1.9e-120
                             TIGR01850   2 kvaivGasGYtGaeLlrllakHpevevtklvssreagkklsevhphlkglvdlkleeleeeeileeadvvflA 74 
                                           ++a+ GasGY+G+e+lrll +Hpe+e+ ++++ ++ag++l ++ phl  l+d  l e+++e +l+ +dvvflA
  NCBI__GCF_000744815.1:WP_037570918.1   4 RAAVAGASGYAGGEVLRLLLQHPEIEIGAVTAGSNAGSRLGALQPHLAPLADRILGETTAE-TLAGHDVVFLA 75 
                                           689*************************9999999*****************888887776.567******** PP

                             TIGR01850  75 lphgvsaelvpellekgvkvidlSadfRlkdaevYekwYgkkhekeelleeavYGlpEln..reeikkaklia 145
                                           lphg+sa+++ e l  +v v+d++adfRl+d++++e++Yg+ h+      ++ YGlpEl   re+++++++ia
  NCBI__GCF_000744815.1:WP_037570918.1  76 LPHGQSAAVA-EQLGPDVLVVDCGADFRLEDPAAWERFYGSAHAG-----TWPYGLPELPgaRERLAGSRRIA 142
                                           *******999.667999**************************99.....8********999*********** PP

                             TIGR01850 146 nPGCyaTaalLalaPllkekliepksiivdaksGvSgAGrkasekslfaevnenlkpYkvt.kHrHtpEieqe 217
                                           +PGCy+Ta+ Lal+P+++++l ep+ ++v a sG+SgAG++a+++ l +ev+++++pY v   HrHtpE++q+
  NCBI__GCF_000744815.1:WP_037570918.1 143 VPGCYPTAVSLALFPAYAAGLAEPQ-AVVVAASGTSGAGKSAKPHLLGSEVMGSMSPYGVGgVHRHTPEMQQN 214
                                           ***********************96.9999******************************989********** PP

                             TIGR01850 218 lsklaekkvkvsftphlvpmtrGilatiyaklkkelteeelrklyeevYedepfvrvlkegelPstkavlgsn 290
                                           ls++a++ v vsftp+l+pm rGilat++a+++ ++t+e+lr++y++vY de+fv++l+eg++P+t av+g n
  NCBI__GCF_000744815.1:WP_037570918.1 215 LSAVAGEPVAVSFTPTLAPMPRGILATCSAQARPGTTAESLRAAYQKVYGDEEFVSLLPEGQWPATAAVYGAN 287
                                           ************************************************************************* PP

                             TIGR01850 291 fvdigvavdeetkrvvvvsaiDNLvKGaagqAvqnlNlmlgfdetegLeklpllp 345
                                           +v+++va+de+ +r++v+saiDNL+KG+ag Avq++Nl+lg++e  gL+k++++p
  NCBI__GCF_000744815.1:WP_037570918.1 288 TVQVQVALDESVGRIIVISAIDNLTKGTAGGAVQSMNLALGLPEGLGLPKIGVAP 342
                                           ***************************************************9988 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (345 nodes)
Target sequences:                          1  (342 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00
# Mc/sec: 15.01
//
[ok]

This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis