Align 2-oxoacid:ferredoxin oxidoreductase 2, subunit alpha; OFOR2; EC 1.2.7.11 (characterized)
to candidate WP_041100122.1 SUTH_RS14075 2-oxoacid:acceptor oxidoreductase subunit alpha
Query= SwissProt::Q9YBX7 (642 letters) >NCBI__GCF_000828635.1:WP_041100122.1 Length = 616 Score = 220 bits (560), Expect = 2e-61 Identities = 190/643 (29%), Positives = 288/643 (44%), Gaps = 68/643 (10%) Query: 3 DISLIIGGPQGGGIESAGQIAIKSMVLLGYEVLGSREYHSNIMGAHSYYHLRVQQHRPRS 62 D + G G SA ++ +S++ +G V + SNI G ++Y +RV Sbjct: 12 DFVIKFANVNGSGSASANELFARSIMRMGVPVAPRNIFPSNIQGMPTWYEMRVSAAGWLG 71 Query: 63 LKLPVDGVLALDAESVFTHFRDVRPGGILVYDPGTKSTRVDAIQPMAGPLKKRLKSLFDS 122 + D ++A++ ++ ++ GG LVYD K+L S Sbjct: 72 RRGGCDLMVAMNPQTWDRDIAEIEAGGYLVYDS--------------------TKALPRS 111 Query: 123 RGMQPVVESAVKLAEEAGARIVGLPLKEMLKTLSERTGAPVARVSRALNTLGLASMLYML 182 R + ++G+PL E+ E T ++ + N + + ++ +L Sbjct: 112 RFRDDIT-------------VLGIPLTEICNR--EYTDPRQRQLFK--NIMYVGALTALL 154 Query: 183 GVPVEYIEKAISLQFAGKEKVINMNVEAVRIAVDYVREAFGEPES-RLPPGPRRGQTMMV 241 G+ +EK I+ Q+ GK+K+I NV A+++ DY RE P R+ G+ + + Sbjct: 155 GMDFTVVEKLIADQYRGKDKLIGANVNALKLGYDYARENVSCPIGLRVERSDGVGEKIFI 214 Query: 242 ATGNDLVAMGKIVGGLGVITYYPITPSSDEALYVEKHSYISIDGPLAEKLGYDK----IA 297 GND +G + GG V +YPITPS+ S I G K D Sbjct: 215 -NGNDACGLGAVYGGATVAGWYPITPST---------SVIEAFGSYCRKYRTDPDNGMAR 264 Query: 298 VAIVQMEDELASINAVLGAAAAGARASTTTSGPGFSLMNEAVSLAVEAEIPVVVTLWMRA 357 AIVQ EDELASI V+GAA GARA T TSGPG SLM E LA AE+PVVV R Sbjct: 265 YAIVQAEDELASIGMVIGAAWNGARAFTATSGPGISLMQEFFGLAYFAEVPVVVFNVQRG 324 Query: 358 GPSTGMPTRTGQQDLLHSIFSGHGDAPKIVLASGDHVEAFYDAIKAFNWAEEFQTPVIHL 417 GPSTGMPTRT Q DL+ ++ HGD ++L D E F +AF+ AE QTPV + Sbjct: 325 GPSTGMPTRTQQSDLIACAYASHGDTKHVLLFPEDPYECFTLGAQAFDLAERLQTPVFVM 384 Query: 418 LDKYLASSMVSLAREDLDPSKVPITRGKLLD----NPPADYRRY-EVVEDGIS----PRA 468 LD + + D D + RGK++ ++ RY +V DGI P Sbjct: 385 LDLDIGMNDRLCQPFDWDDQR-RYDRGKVMTAEELEAGKNFGRYLDVDGDGIPWRTIPGT 443 Query: 469 RLGSATMVITGLEHDEYGYATEDPVMREIMMFKRERKFKVIEERIPDEEKAVLHGDSEAS 528 G + Y +E M + RKF+ ++ +P V Sbjct: 444 HPSKGAFFSRGTTRNAYAKYSEAGTDYVYNMERLRRKFETAKKLVP--APMVQRASQATR 501 Query: 529 VALVSFGSTKQPILEALEMLRDEGVRARFAQVRLLYPFPGRLVEEMLEGVEKVIMVEQNL 588 + +GST + EA +L +G+ +VR +PF ++E + E + V +VEQN Sbjct: 502 FGAIYYGSTSPAMAEAYALLEAQGIHVDIMRVR-GFPFADEVIEFINEH-DHVFVVEQNE 559 Query: 589 LGQLAMLLRAHTSIKPDS--SIVKINGRPLYSFEVAGAVKRIL 629 GQL MLL + P ++ +G P+ + ++G + L Sbjct: 560 SGQLRMLLMNEGEVDPKRLIRVLHYDGTPITARFISGRIAAAL 602 Lambda K H 0.318 0.135 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 831 Number of extensions: 42 Number of successful extensions: 6 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 2 Number of HSP's successfully gapped: 2 Length of query: 642 Length of database: 616 Length adjustment: 38 Effective length of query: 604 Effective length of database: 578 Effective search space: 349112 Effective search space used: 349112 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 54 (25.4 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory