Align Methionine synthase component, B12 binding and B12-binding cap domains (EC:2.1.1.13) (characterized)
to candidate WP_041101157.1 SUTH_RS17740 methionine synthase
Query= reanno::Phaeo:GFF1319 (233 letters) >NCBI__GCF_000828635.1:WP_041101157.1 Length = 1231 Score = 108 bits (269), Expect = 6e-28 Identities = 63/184 (34%), Positives = 101/184 (54%), Gaps = 6/184 (3%) Query: 15 DEDLVQQMFDDLYDGLKEEIEESVNILLERGWAPYKVLTEALVGGMTIVGADFRDGILFV 74 D+ L M + + + + EE L G P V+ L+GGM +VG F G +F+ Sbjct: 659 DKRLEHAMVKGITEYVVADTEECRAALAAAGKPPLSVIEGPLMGGMNVVGDLFGAGKMFL 718 Query: 75 PEVLLAANAMKGGMAILKPLLAE----TGAPRMGSMVIGTVKGDIHDIGKNLVSMMMEGA 130 P+V+ +A MK +A L P + E TG+ G +VI TVKGD+HDIGKN+V +++ Sbjct: 719 PQVVKSARVMKQAVAHLIPWIEEEKKRTGSTSKGRIVIATVKGDVHDIGKNIVGVVLGCN 778 Query: 131 GFEVVDIGINNPVENYLEALEEHQPDILGMSALLTTTMPYMKVVIDTMIEQGKRDDYIVL 190 G++++D+G+ + L A +EH +G+S L+T ++ M + M QG +L Sbjct: 779 GYDIIDLGVMVSADKILHAAKEHGAQAIGLSGLITPSLEEMSHIASEMQRQGFTQP--LL 836 Query: 191 VGGA 194 +GGA Sbjct: 837 IGGA 840 Lambda K H 0.318 0.138 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 618 Number of extensions: 34 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 233 Length of database: 1231 Length adjustment: 35 Effective length of query: 198 Effective length of database: 1196 Effective search space: 236808 Effective search space used: 236808 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 52 (24.6 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory