Align Bifunctional glyoxylate cycle protein; Gex-3-interacting protein 7; EC 4.1.3.1; EC 2.3.3.9 (characterized)
to candidate WP_041303752.1 BTUS_RS05060 malate synthase A
Query= SwissProt::Q10663 (968 letters) >NCBI__GCF_000092905.1:WP_041303752.1 Length = 534 Score = 576 bits (1485), Expect = e-168 Identities = 291/512 (56%), Positives = 365/512 (71%), Gaps = 8/512 (1%) Query: 455 KILTPDALRFLHDLNTEFNPRRLRLLSKRNQVQADINNSLWFPDFNKETEVLRSDQGWKG 514 +ILTP AL F+ DL+ +FN RR LL++R + QA I+ PDF ET LR W Sbjct: 23 EILTPGALEFVADLHRQFNGRRQALLAERQKRQAAIDGGQ-MPDFPAETAELRQAD-WTV 80 Query: 515 AEIPRDLQDRRVEITGPT-DRKMVINAMNSGANVFMADFEDSNSPTWRNQLEGQINLYDA 573 A IP DL DRRVEITGP DRKMVINA+NSGA FMADFED+NSPTW N + GQINL DA Sbjct: 81 APIPADLLDRRVEITGPAGDRKMVINALNSGAKTFMADFEDANSPTWENTVGGQINLRDA 140 Query: 574 VRNNISYTHPTTKKEYTLNEKHAVLKVRPRGWHLPEKHVLIHNQPTSGSLFDFGLFVFHN 633 VR I Y P K+ Y L ++ AVL VRPRGWHLPEKHVL+ +P SG+LFDFGL+ FHN Sbjct: 141 VRRTIRYVSPEGKR-YELKDRTAVLIVRPRGWHLPEKHVLVDGEPISGALFDFGLYFFHN 199 Query: 634 AKALIAQGSGPYFYLPKLQSAEEAQLWADVFKYTEDKLGLARGTIKCTVLIEHLLASFQL 693 + L+ +G+GPYFYLPK++S EA+LW DVF +D+LG+ RGTIK TVLIE +LA+F++ Sbjct: 200 VRELLERGTGPYFYLPKMESRFEARLWNDVFNVAQDRLGIPRGTIKATVLIETILAAFEM 259 Query: 694 HEIIHALKDNIVGLNCGRWDYIFSYIKTFQNHRKFLLPDRFQIGMTAPFMRNYSLEVIKA 753 EI++ L+D+ GLNCGRWDYIFS+IK F+N + +LPDR + MTAPFMR Y+ IK Sbjct: 260 DEILYELRDHAAGLNCGRWDYIFSFIKRFRNRPEVILPDRGLVTMTAPFMRAYTQLTIKT 319 Query: 754 CHLRGIHAMGGMAAQIPIKHDQVANDKAFALVRADKEREATDGHDGTWVAHPGLVPLAKR 813 CH RG A+GGMAAQIP+K+D AN++AFA VRADKEREATDGHDGTWVAHPGLVP+A Sbjct: 320 CHRRGAMAIGGMAAQIPVKNDPAANEEAFAKVRADKEREATDGHDGTWVAHPGLVPVAME 379 Query: 814 VFDQMMPKPNQISKNLTRANCTKEDLTVIPEGTRTEAGFRHNISVTLGYLDSWLRGTGCV 873 VFD++MP PNQ+ + + + DL +PEG TE G R NISV + Y+++WLRG+G V Sbjct: 380 VFDRLMPTPNQLDRKREDVHVSAADLIRVPEGPITEQGLRTNISVGIQYIEAWLRGSGAV 439 Query: 874 PLYNLMEDAATAEISRAQLWQWLHHD-AKLEDGRTIDAGLVKQTIAAETERRLIRAGS-- 930 P++NLMEDAATAEISRAQ+WQW+ H LEDGR + L +Q + E + G Sbjct: 440 PIFNLMEDAATAEISRAQVWQWIRHPRGVLEDGRKVTVELFRQVLDEELNKIKAELGEDR 499 Query: 931 -VVNRIPEAADLLEKFVTEEKMSDFLTTDAYD 961 R EAA+L + T + ++FLT Y+ Sbjct: 500 FAGGRYSEAAELFSRLTTSDSFAEFLTLPGYE 531 Lambda K H 0.319 0.134 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1239 Number of extensions: 69 Number of successful extensions: 5 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 968 Length of database: 534 Length adjustment: 40 Effective length of query: 928 Effective length of database: 494 Effective search space: 458432 Effective search space used: 458432 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 55 (25.8 bits)
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory