Alignments for a candidate for asp-kinase in Chlorobaculum parvum NCIB 8327

GapMind for Amino acid biosynthesis

Alignments for a candidate for asp-kinase in Chlorobaculum parvum NCIB 8327

Align aspartate kinase (EC 2.7.2.4) (characterized)
to candidate WP_041466196.1 CPAR_RS10150 lysine-sensitive aspartokinase 3

Query= BRENDA::Q57991
         (473 letters)



>NCBI__GCF_000020505.1:WP_041466196.1
          Length = 470

 Score =  308 bits (788), Expect = 3e-88
 Identities = 191/474 (40%), Positives = 281/474 (59%), Gaps = 32/474 (6%)

Query: 4   VMKFGGTSVGSGERIRHVAKIVTKRKKEDDDVVVVVSAMSEVTNALVEISQQALDVRDIA 63
           VMKFGGTSVG+   +R V   + ++KK    +VV+ SA S +TN L++I+ +A   R + 
Sbjct: 3   VMKFGGTSVGTAAAMRQVIANIAEKKKTSAPLVVL-SACSGITNKLIQIADEAGSGR-LK 60

Query: 64  KVGDFIKFIREKHYKAIEEAIKSEEIKEEVKKIIDSRIEELEKVLIGVAYLGELTPKSRD 123
           +    +  +R+ H   I E I +EE++  V + I   +  LE++  G+  +GELT +SRD
Sbjct: 61  EALKLVGEVRQFHLDLIGELIGNEELRAAVIEKIGVYLTRLERLTEGIEIVGELTERSRD 120

Query: 124 YILSFGERLSSPILSGAIRDLGEKSIALEGGEAGIITDNNFGSAR----VKRLEVKERLL 179
              SFGE LS+ + + A+ + G     L+     +ITD+ +G AR      R    E + 
Sbjct: 121 RFCSFGELLSTSVFAAALNEAGVPCEWLDVRTV-MITDDRYGFARPLAETCRKNTTEIIK 179

Query: 180 PLLKEGIIPVVTGFIGTTEEGYITTLGRGGSDYSAALIGYGLDADIIEIWTDVSGVYTTD 239
           PLL  G + V  G+IG+TE G  TTLGRGGSD SAAL G  L ++ IEIWTDV GV TTD
Sbjct: 180 PLLDAGTVVVTQGYIGSTESGRTTTLGRGGSDLSAALFGAWLHSESIEIWTDVDGVMTTD 239

Query: 240 PRLVPTARRIPKLSYIEAMELAYFGAKVLHPRTIEPAMEKGIPILVKNTFEPESEGTLIT 299
           PR+VP AR I  +++ EA ELAY GAKVLHP TI PA+EK IP+ V NT+ P+S+GTLIT
Sbjct: 240 PRMVPEARSIRVMTFSEAAELAYLGAKVLHPDTIAPAVEKNIPVFVLNTWHPDSKGTLIT 299

Query: 300 NDMEM-----SDSIVKAISTIKNVALINIFGAGMVGVSGTAARIFKALGEEEVNVILISQ 354
           ND E+        +VK+I+  K  A++NI    M G  G  + +F       ++V +IS 
Sbjct: 300 NDPELLAGKSHGGLVKSIAVKKGQAILNIRSNRMFGRHGFMSELFDVFERFAISVEMIS- 358

Query: 355 GSSETNISLVVSEEDVDKA-LKALKREFGDFGKKSFLNNNLIRDVSVDKDVCVISVVGAG 413
            +SE ++SL V +  V +  +KAL               + + +V ++  V  +SVVG  
Sbjct: 359 -TSEVSVSLTVDDGSVGETFIKAL---------------SSLGEVEIEHKVATVSVVGDN 402

Query: 414 MRGAKGIAGKIFTAVSESGANIKMIAQGSSEVNISFVIDEKDLLNCVRKLHEKF 467
           +R ++G+AG+IF ++     N++MI+QG+SE+N+  V+DE D+   V  LH +F
Sbjct: 403 LRMSRGVAGRIFNSL--RNVNLRMISQGASEINVGVVVDESDVAPAVAALHCEF 454


Lambda     K      H
   0.316    0.135    0.364 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 523
Number of extensions: 23
Number of successful extensions: 8
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 473
Length of database: 470
Length adjustment: 33
Effective length of query: 440
Effective length of database: 437
Effective search space:   192280
Effective search space used:   192280
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 51 (24.3 bits)

Align candidate WP_041466196.1 CPAR_RS10150 (lysine-sensitive aspartokinase 3)
to HMM TIGR00657 (aspartate kinase (EC 2.7.2.4))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00657.hmm
# target sequence database:        /tmp/gapView.6914.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00657  [M=442]
Accession:   TIGR00657
Description: asp_kinases: aspartate kinase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   3.2e-122  394.7   1.4   3.6e-122  394.5   1.4    1.0  1  lcl|NCBI__GCF_000020505.1:WP_041466196.1  CPAR_RS10150 lysine-sensitive as


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000020505.1:WP_041466196.1  CPAR_RS10150 lysine-sensitive aspartokinase 3
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  394.5   1.4  3.6e-122  3.6e-122       4     441 ..       2     455 ..       1     456 [. 0.95

  Alignments for each domain:
  == domain 1  score: 394.5 bits;  conditional E-value: 3.6e-122
                                 TIGR00657   4 iVqKFGGtSvgnverikkvakivkkekekgnqvvVVvSAmagvTdaLvelaekvsseee...kelieki 69 
                                               +V+KFGGtSvg++  +++v   + ++k k   ++VV+SA +g+T++L+++a+++ s++     +l+ ++
  lcl|NCBI__GCF_000020505.1:WP_041466196.1   2 VVMKFGGTSVGTAAAMRQVIANIAEKK-KTSAPLVVLSACSGITNKLIQIADEAGSGRLkeaLKLVGEV 69 
                                               9******************99998888.5559*************************996666677899 PP

                                 TIGR00657  70 rekhlealeela.sqalkeklkallekeleevkk............ereldlilsvGEklSaallaaal 125
                                               r+ hl+ + el+ +++l++ + + +   l+ +++            er++d+  s+GE lS++++aaal
  lcl|NCBI__GCF_000020505.1:WP_041466196.1  70 RQFHLDLIGELIgNEELRAAVIEKIGVYLTRLERltegieivgeltERSRDRFCSFGELLSTSVFAAAL 138
                                               **************999999999998888888888999******************************* PP

                                 TIGR00657 126 eelgvkavsllgaeagiltdsefgrAk....vleeikterleklleegiivvvaGFiGatekgeittLG 190
                                               +e g    ++l+ + +++td+++g A+    + ++ +te +++ll++g++vv++G+iG+te+g++ttLG
  lcl|NCBI__GCF_000020505.1:WP_041466196.1 139 NEAG-VPCEWLDVRTVMITDDRYGFARplaeTCRKNTTEIIKPLLDAGTVVVTQGYIGSTESGRTTTLG 206
                                               ****.99********************99988899999******************************* PP

                                 TIGR00657 191 RGGSDltAallAaalkAdeveiytDVdGiytaDPrivpeArrldeisyeEalELaslGakvLhprtlep 259
                                               RGGSDl+Aal +a l+ + +ei+tDVdG++t+DPr+vpeAr +  +++ Ea+ELa+lGakvLhp+t+ p
  lcl|NCBI__GCF_000020505.1:WP_041466196.1 207 RGGSDLSAALFGAWLHSESIEIWTDVDGVMTTDPRMVPEARSIRVMTFSEAAELAYLGAKVLHPDTIAP 275
                                               ********************************************************************* PP

                                 TIGR00657 260 amrakipivvkstfnpeaeGTlivaksk....seeepavkalsldknqalvsvsgttmk..pgilaevf 322
                                               a++++ip++v +t+ p+++GTli+++ +    +++   vk+++++k qa+++++++ m    g+++e+f
  lcl|NCBI__GCF_000020505.1:WP_041466196.1 276 AVEKNIPVFVLNTWHPDSKGTLITNDPEllagKSHGGLVKSIAVKKGQAILNIRSNRMFgrHGFMSELF 344
                                               ********************************9999**********************999******** PP

                                 TIGR00657 323 galaeakvnvdlilqsssetsisfvvdkedadkakellkkkvkeekaleevevekklalvslvGagmks 391
                                                  ++  ++v++i+  +se s+s++vd+ ++ ++       +k+++ l+eve+e+k+a+vs+vG++++ 
  lcl|NCBI__GCF_000020505.1:WP_041466196.1 345 DVFERFAISVEMIS--TSEVSVSLTVDDGSVGETF------IKALSSLGEVEIEHKVATVSVVGDNLRM 405
                                               **************..88889*****988765443......457899********************** PP

                                 TIGR00657 392 apgvaakifeaLaeeniniemis..sseikisvvvdekdaekavealheklv 441
                                                 gva++if+ L++  +n +mis  +sei++ vvvde+d+  av alh +++
  lcl|NCBI__GCF_000020505.1:WP_041466196.1 406 SRGVAGRIFNSLRN--VNLRMISqgASEINVGVVVDESDVAPAVAALHCEFF 455
                                               ************98..9******99***********************9997 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (442 nodes)
Target sequences:                          1  (470 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.02
# Mc/sec: 8.89
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis