GapMind for Amino acid biosynthesis

 

Alignments for a candidate for hisE in Persephonella marina EX-H1

Align putative bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase (EC 3.5.4.19; EC 3.6.1.31) (characterized)
to candidate WP_041530984.1 PERMA_RS01820 bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE

Query= metacyc::HISTCYCLOPRATPPHOS
         (203 letters)



>NCBI__GCF_000021565.1:WP_041530984.1
          Length = 206

 Score =  166 bits (419), Expect = 4e-46
 Identities = 89/195 (45%), Positives = 119/195 (61%), Gaps = 8/195 (4%)

Query: 17  GLMPVIVQHAVSGEVLMLGYMNPEALDKTLESGKVTFFSRTKQRLWTKGETSGNFLNVVS 76
           GL+PVI Q   SG+VLM  Y N EA+ KT+E+G  T++SR++  LW KGETSGN   VV 
Sbjct: 12  GLVPVITQSYYSGKVLMQAYANREAILKTVETGFATYYSRSRNELWIKGETSGNKQRVVD 71

Query: 77  IAPDCDNDTLLVLANPIGPTCHKGTSSCF--------GDTAHQWLFLYQLEQLLAERKSA 128
           I  DCD D++L L    G  CH G  SCF         +    +  L+ L + + ERK  
Sbjct: 72  IRVDCDGDSVLYLVVDYGVACHTGKESCFYRDIDFKEKEKPDPYEILHALYKKIEERKEK 131

Query: 129 DPETSYTAKLYASGTKRIAQKVGEEGVETALAATVHDRFELTNEASDLMYHLLVLLQDQG 188
            PE SY AKL++ G+ +I QKVGEE VET +A    D+ E+  EASDL++HLL+ L D G
Sbjct: 132 RPEGSYVAKLFSKGSDKIIQKVGEEAVETVIALKNRDKNEIVYEASDLVFHLLIALVDSG 191

Query: 189 LDLTTVIENLRKRHQ 203
           + L  + E L KR++
Sbjct: 192 VKLEDIQEELLKRYK 206


Lambda     K      H
   0.317    0.132    0.384 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 100
Number of extensions: 4
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 203
Length of database: 206
Length adjustment: 21
Effective length of query: 182
Effective length of database: 185
Effective search space:    33670
Effective search space used:    33670
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 45 (21.9 bits)

Align candidate WP_041530984.1 PERMA_RS01820 (bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE)
to HMM PF01502 (PRA-CH)

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/PF01502.22.hmm
# target sequence database:        /tmp/gapView.24472.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       PRA-CH  [M=74]
Accession:   PF01502.22
Description: Phosphoribosyl-AMP cyclohydrolase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
    3.6e-37  112.4   0.2    6.6e-37  111.6   0.2    1.4  1  lcl|NCBI__GCF_000021565.1:WP_041530984.1  PERMA_RS01820 bifunctional phosp


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000021565.1:WP_041530984.1  PERMA_RS01820 bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  111.6   0.2   6.6e-37   6.6e-37       1      74 []      28     101 ..      28     101 .. 0.99

  Alignments for each domain:
  == domain 1  score: 111.6 bits;  conditional E-value: 6.6e-37
                                    PRA-CH   1 mlaymneealektletgkavyySrsrqklwkkGetsgnvqkvkeirldcDeDalllkveqkgaaCHtge 69 
                                               m ay+n+ea+ kt+etg a+yySrsr++lw kGetsgn q+v++ir+dcD+D++l+ v   g aCHtg+
  lcl|NCBI__GCF_000021565.1:WP_041530984.1  28 MQAYANREAILKTVETGFATYYSRSRNELWIKGETSGNKQRVVDIRVDCDGDSVLYLVVDYGVACHTGK 96 
                                               99******************************************************************* PP

                                    PRA-CH  70 rsCFy 74 
                                               +sCFy
  lcl|NCBI__GCF_000021565.1:WP_041530984.1  97 ESCFY 101
                                               ****7 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (74 nodes)
Target sequences:                          1  (206 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.00
# Mc/sec: 5.74
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory