Alignments for a candidate for dapF in Xanthobacter autotrophicus Py2

GapMind for Amino acid biosynthesis

Alignments for a candidate for dapF in Xanthobacter autotrophicus Py2

Align Diaminopimelate epimerase; DAP epimerase; EC 5.1.1.7; PLP-independent amino acid racemase (uncharacterized)
to candidate WP_041575065.1 XAUT_RS02085 diaminopimelate epimerase

Query= curated2:Q8YJF0
         (303 letters)



>NCBI__GCF_000017645.1:WP_041575065.1
          Length = 285

 Score =  285 bits (728), Expect = 1e-81
 Identities = 148/276 (53%), Positives = 185/276 (67%), Gaps = 6/276 (2%)

Query: 7   FARMNGLGNQIIVADMRGRADSITSAAAIRLASDSETAFDQIMAIHDPRTPGTDYYIAII 66
           F +MNGLGN+I+V D+R     + +AAA  LA  S   FDQ M ++ PR  GT  ++ I+
Sbjct: 9   FVKMNGLGNEILVLDLRADPVEVPAAAARALARPSVLPFDQAMVLYPPRREGTAAFVRIL 68

Query: 67  NCDGTQAQACGNGTRCVVQALAAETGRHAFTFETRAGILTATEHDDGLISVDMGTPRFDW 126
           N DG+ + ACGNGTRC+    A  TG     FE+ AG+L      DG + VDMG PRFDW
Sbjct: 69  NSDGSFSAACGNGTRCIAALEAERTGAPHALFESEAGLLDCLVRPDGQVEVDMGAPRFDW 128

Query: 127 QDIPLAQAVADTRKIELQVDPADAPVLHSPSIASMGNPHAVFWVDKDVWSYELDKFGPLL 186
           +DIPLA+ VA+T ++ +    A  P     S+ SMGNPHAVF+V  D  + +++K G  L
Sbjct: 129 RDIPLARDVAETAQVIVPGFEALGPT----SLVSMGNPHAVFFV-PDANAVDVEKLGAAL 183

Query: 187 ENHPIFPERANISIAHVTSSDTIDLRTWERGAGLTRACGSAACAAAVSAARTGRTGRKVT 246
           E+HP+FPERANIS A +T+ D I L  WERGAG TRACG+AACA  VSAARTGRTGR VT
Sbjct: 184 EHHPLFPERANISFASLTAPDRILLHVWERGAGRTRACGTAACATGVSAARTGRTGRSVT 243

Query: 247 VNVPGGPLLIEWRD-DDHVMMTGPAEWEFSGTFDPA 281
           V +PGG L I WR+ D HV+MTGP E EFSGT  PA
Sbjct: 244 VTLPGGDLEISWREADGHVLMTGPVEHEFSGTLSPA 279


Lambda     K      H
   0.318    0.132    0.408 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 297
Number of extensions: 12
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 303
Length of database: 285
Length adjustment: 26
Effective length of query: 277
Effective length of database: 259
Effective search space:    71743
Effective search space used:    71743
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 48 (23.1 bits)

Align candidate WP_041575065.1 XAUT_RS02085 (diaminopimelate epimerase)
to HMM TIGR00652 (dapF: diaminopimelate epimerase (EC 5.1.1.7))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00652.hmm
# target sequence database:        /tmp/gapView.15205.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00652  [M=270]
Accession:   TIGR00652
Description: DapF: diaminopimelate epimerase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
    1.8e-68  216.9   0.0      2e-68  216.7   0.0    1.0  1  lcl|NCBI__GCF_000017645.1:WP_041575065.1  XAUT_RS02085 diaminopimelate epi


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000017645.1:WP_041575065.1  XAUT_RS02085 diaminopimelate epimerase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  216.7   0.0     2e-68     2e-68       3     268 ..       9     276 ..       7     278 .. 0.92

  Alignments for each domain:
  == domain 1  score: 216.7 bits;  conditional E-value: 2e-68
                                 TIGR00652   3 FlkmhGlgNdFvlvdevdeelvkeeaelvrkvcdrhtgvgaDgvllvep.sseeadvklrifNsDGSea 70 
                                               F+km+GlgN+ +++d +  + v+  ++ +++  +r   + +D+ +++ p   e + + +ri NsDGS  
  lcl|NCBI__GCF_000017645.1:WP_041575065.1   9 FVKMNGLGNEILVLDLRA-DPVEV-PAAAARALARPSVLPFDQAMVLYPpRREGTAAFVRILNSDGSFS 75 
                                               9*********99999887.44444.7788888889999*****9999984566677778********** PP

                                 TIGR00652  71 emCGNgiRcfakfvyekglkekkelsvetlaglikveveeenkkvkvdmgepkfkkeeipltvekeeek 139
                                               + CGNg Rc+a+  +e++++   +  +e++agl+ + v  ++ +v+vdmg p+f   +ipl  + +e+ 
  lcl|NCBI__GCF_000017645.1:WP_041575065.1  76 AACGNGTRCIAALEAERTGA--PHALFESEAGLLDCLVRPDG-QVEVDMGAPRFDWRDIPLARDVAETA 141
                                               ********************..7999****************.*******************9999998 PP

                                 TIGR00652 140 eellalev.l....vvdvGnPHlvvfvedvekldleelgklleaheefpegvNvefvevkkedeiklrv 203
                                               ++++     l    +v++GnPH+v+fv d + +d+e+lg++le+h+ fpe+ N+ f+  + +d+i l+v
  lcl|NCBI__GCF_000017645.1:WP_041575065.1 142 QVIVPGFEaLgptsLVSMGNPHAVFFVPDANAVDVEKLGAALEHHPLFPERANISFASLTAPDRILLHV 210
                                               88887544435567******************************************************* PP

                                 TIGR00652 204 yERGageTlaCGtGavAsavvalklgktkkkvtvhleggeLeievkedg.kvyltGpavlvlegel 268
                                               +ERGag T+aCGt a+A+ v a+++g+t ++vtv+l+gg+Lei+++e++ +v++tGp+++ ++g+l
  lcl|NCBI__GCF_000017645.1:WP_041575065.1 211 WERGAGRTRACGTAACATGVSAARTGRTGRSVTVTLPGGDLEISWREADgHVLMTGPVEHEFSGTL 276
                                               *********************************************99888**********999987 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (270 nodes)
Target sequences:                          1  (285 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 7.08
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis