Alignments for a candidate for PAH in Burkholderia vietnamiensis G4

GapMind for Amino acid biosynthesis

Alignments for a candidate for PAH in Burkholderia vietnamiensis G4

Align phenylalanine 4-monooxygenase (EC 1.14.16.1) (characterized)
to candidate WP_043291603.1 phenylalanine 4-monooxygenase

Query= BRENDA::P30967
         (297 letters)



>NCBI__GCF_000016205.1:WP_043291603.1
          Length = 297

 Score =  343 bits (879), Expect = 4e-99
 Identities = 170/275 (61%), Positives = 200/275 (72%), Gaps = 4/275 (1%)

Query: 26  DFTLPQPLDRYSAEDHATWATLYQRQCKLLPGRACDEFMEGLERLEVDADRVPDFNKLNQ 85
           DFT+ QPL+RY   DHA W  LY RQ  LL GRACD F+ GL ++ + ADRVP F  +N+
Sbjct: 22  DFTIDQPLERYGQVDHAVWQQLYARQAALLRGRACDAFIGGLGKIRLPADRVPSFADVNR 81

Query: 86  KLMAATGWKIVAVPGLIPDDVFFEHLANRRFPVTWWLREPHQLDYLQEPDVFHDLFGHVP 145
           +L  ATGW+IVAVPGL+PD VFFEHLA+RRFPVTWW+R P QLDYLQEPD FHDLFGHVP
Sbjct: 82  QLKPATGWEIVAVPGLVPDRVFFEHLAHRRFPVTWWMRRPDQLDYLQEPDCFHDLFGHVP 141

Query: 146 LLINPVFADYLEAYGKGGVK-AKALGALPMLARLYWYTVEFGLINTPA---GMRIYGAGI 201
           LLI PVFADY++AYG+  +  A    AL  LARLYWYTVEFGLI  P    G+ IYGAGI
Sbjct: 142 LLIEPVFADYMQAYGRTALAVADDDAALARLARLYWYTVEFGLIRDPRGTNGLSIYGAGI 201

Query: 202 LSSKSESIYCLDSASPNRVGFDLMRIMNTRYRIDTFQKTYFVIDSFKQLFDATAPDFAPL 261
           +SSK ES+Y ++SA+PNR+GFDL R+M T+YRIDTFQKTYFVID F QLF     D   L
Sbjct: 202 VSSKGESLYSVESAAPNRLGFDLERVMRTKYRIDTFQKTYFVIDDFAQLFALADVDARAL 261

Query: 262 YLQLADAQPWGAGDVAPDDLVLNAGDRQGWADTED 296
             +LA      AG V   D+VL  G  +GW D +D
Sbjct: 262 AERLAALPELSAGTVLDTDVVLQRGTGEGWPDDDD 296


Lambda     K      H
   0.323    0.140    0.445 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 364
Number of extensions: 18
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 297
Length of database: 297
Length adjustment: 26
Effective length of query: 271
Effective length of database: 271
Effective search space:    73441
Effective search space used:    73441
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 48 (23.1 bits)

Align candidate WP_043291603.1 (phenylalanine 4-monooxygenase)
to HMM TIGR01267 (phhA: phenylalanine-4-hydroxylase (EC 1.14.16.1))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01267.hmm
# target sequence database:        /tmp/gapView.10711.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01267  [M=248]
Accession:   TIGR01267
Description: Phe4hydrox_mono: phenylalanine-4-hydroxylase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   1.3e-111  357.7   0.0   1.6e-111  357.4   0.0    1.0  1  lcl|NCBI__GCF_000016205.1:WP_043291603.1  phenylalanine 4-monooxygenase


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000016205.1:WP_043291603.1  phenylalanine 4-monooxygenase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  357.4   0.0  1.6e-111  1.6e-111       1     241 [.      22     267 ..      22     273 .. 0.97

  Alignments for each domain:
  == domain 1  score: 357.4 bits;  conditional E-value: 1.6e-111
                                 TIGR01267   1 dftvaqdldryseeehavwatlidrqlkllegracdeyldGveklgldadripdleevneklraltGwk 69 
                                               dft++q+l+ry + +havw+ l+ rq+ ll+gracd ++ G+ k+ l+adr+p + +vn++l+ +tGw+
  lcl|NCBI__GCF_000016205.1:WP_043291603.1  22 DFTIDQPLERYGQVDHAVWQQLYARQAALLRGRACDAFIGGLGKIRLPADRVPSFADVNRQLKPATGWE 90 
                                               79******************************************************************* PP

                                 TIGR01267  70 ivavpglipadvffehlanrrfpvttflrtpeeldylqepdvfhdlfGhvpllsnpvfadfleayGkkg 138
                                               ivavpgl+p+ vffehla rrfpvt+++r p++ldylqepd fhdlfGhvpll++pvfad+++ayG+++
  lcl|NCBI__GCF_000016205.1:WP_043291603.1  91 IVAVPGLVPDRVFFEHLAHRRFPVTWWMRRPDQLDYLQEPDCFHDLFGHVPLLIEPVFADYMQAYGRTA 159
                                               ********************************************************************* PP

                                 TIGR01267 139 vkakalgaa..llarlywytvefGlvetaag...lriyGaGilsskkelvyaleskeplrvafdllevm 202
                                               + +   +aa   larlywytvefGl++++ g   l iyGaGi+ssk e++y++es+ p+r +fdl++vm
  lcl|NCBI__GCF_000016205.1:WP_043291603.1 160 LAVADDDAAlaRLARLYWYTVEFGLIRDPRGtngLSIYGAGIVSSKGESLYSVESAAPNRLGFDLERVM 228
                                               ***999988678***************987644589********************************* PP

                                 TIGR01267 203 rtryridklqkayfvlpslkrlfdaaqedfealvaeakd 241
                                               rt+yrid++qk+yfv++++ +lf +a+ d  al  +++ 
  lcl|NCBI__GCF_000016205.1:WP_043291603.1 229 RTKYRIDTFQKTYFVIDDFAQLFALADVDARALAERLAA 267
                                               *********************************998765 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (248 nodes)
Target sequences:                          1  (297 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.00
# Mc/sec: 12.12
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis