Alignments for a candidate for asp-kinase in Thiomicrospira microaerophila ASL8-2

GapMind for Amino acid biosynthesis

Alignments for a candidate for asp-kinase in Thiomicrospira microaerophila ASL8-2

Align Aspartate kinase Ask_LysC; Aspartokinase; EC 2.7.2.4 (characterized)
to candidate WP_044409599.1 NA59_RS06170 aspartate kinase

Query= SwissProt::A4VJB4
         (412 letters)



>NCBI__GCF_000934765.1:WP_044409599.1
          Length = 405

 Score =  533 bits (1373), Expect = e-156
 Identities = 275/404 (68%), Positives = 335/404 (82%), Gaps = 1/404 (0%)

Query: 1   MALIVQKFGGTSVGTVERIEQVAEKVKKFRDGGDDIVVVVSAMSGETNRLIDLAKQISEQ 60
           MALIVQK+GGTSVG++ERI+ VA+KV KF D G  +VVV+SAMSGETNRL ++AK +  +
Sbjct: 1   MALIVQKYGGTSVGSLERIQNVAKKVSKFVDEGHQVVVVLSAMSGETNRLTEMAKTMQAR 60

Query: 61  PVPRELDVMVSTGEQVTIALLAMALIKRGVPAVSYTGNQVRILTDSAHTKARILQIDAQR 120
           P  RE+D++++TGEQVTI+LL+MAL ++G  A+SYTG Q+ I TD  HTKARI  I+A +
Sbjct: 61  PSKREMDMLLTTGEQVTISLLSMALQEKGYGAISYTGWQIPIQTDEVHTKARIDSINADK 120

Query: 121 IQRDIKAGRVVVVAGFQGVDEKGNITTLGRGGSDTTGVALAAALKADECQIYTDVDGVYT 180
           I   +  G+VVVVAGFQGV   G+I+TLGRGGSDTT VALAAALKADECQIYTDVDGVYT
Sbjct: 121 IHDQLNQGKVVVVAGFQGVTANGDISTLGRGGSDTTAVALAAALKADECQIYTDVDGVYT 180

Query: 181 TDPRVVAKAQRLDKITFEEMLEMASLGSKVLQIRAVEFAGKYSVPLRVLHSFQEGPGTLI 240
           TDPRVV +A+RLD ITF+EMLEMASLG+KVLQIR+VEFA KY VPLRVL S  EG GTLI
Sbjct: 181 TDPRVVPEARRLDTITFDEMLEMASLGAKVLQIRSVEFASKYKVPLRVLSSLTEGGGTLI 240

Query: 241 TLDEEESMEQPIISGIAFNRDEAKLTIRGVPDTPGVAFKILGPISAANVEVDMIVQNVAH 300
           T  E+ +ME+P+ISGIAFNRDE+KL IRGVPD PG+AF +LGPI+ AN+E+DMI+QN   
Sbjct: 241 T-SEDNNMEKPLISGIAFNRDESKLQIRGVPDKPGIAFALLGPIADANIEIDMIIQNQGQ 299

Query: 301 DNTTDFTFTVHRNDYNNALQVLQGIAAEMGAREAIGDTNIAKVSIVGVGMRSHAGVASRM 360
           D TTDFTFTV R D  +A ++L+GIA  +GARE I D +IAKVS+VGVGMRSH G+ASRM
Sbjct: 300 DGTTDFTFTVPRGDRAHAHEILEGIAQVLGAREVIFDDSIAKVSLVGVGMRSHVGIASRM 359

Query: 361 FEALAKENINIQMISTSEIKVSVVIEEKYLELAVRALHTAFELD 404
           F+ LA +NINIQMI+T+EIK+SVVI+E  LE AV++LH  FELD
Sbjct: 360 FKVLADQNINIQMIATTEIKISVVIKESELESAVKSLHQQFELD 403


Lambda     K      H
   0.317    0.133    0.357 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 503
Number of extensions: 10
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 412
Length of database: 405
Length adjustment: 31
Effective length of query: 381
Effective length of database: 374
Effective search space:   142494
Effective search space used:   142494
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 50 (23.9 bits)

Align candidate WP_044409599.1 NA59_RS06170 (aspartate kinase)
to HMM TIGR00656 (aspartate kinase, monofunctional class (EC 2.7.2.4))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00656.hmm
# target sequence database:        /tmp/gapView.9275.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00656  [M=407]
Accession:   TIGR00656
Description: asp_kin_monofn: aspartate kinase, monofunctional class
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   1.7e-141  457.8  10.4   1.9e-141  457.7  10.4    1.0  1  lcl|NCBI__GCF_000934765.1:WP_044409599.1  NA59_RS06170 aspartate kinase


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000934765.1:WP_044409599.1  NA59_RS06170 aspartate kinase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  457.7  10.4  1.9e-141  1.9e-141       1     406 [.       1     402 [.       1     403 [. 0.98

  Alignments for each domain:
  == domain 1  score: 457.7 bits;  conditional E-value: 1.9e-141
                                 TIGR00656   1 veliVqKFGGtsvgsserikkaakivlkelkegkkvvVVvSAmskvtdelvelaellklleaisdeisp 69 
                                               + liVqK+GGtsvgs eri+++ak+v k + eg++vvVV+SAms++t++l e+a      ++++  +s+
  lcl|NCBI__GCF_000934765.1:WP_044409599.1   1 MALIVQKYGGTSVGSLERIQNVAKKVSKFVDEGHQVVVVLSAMSGETNRLTEMA------KTMQARPSK 63 
                                               579***************************************************......9******** PP

                                 TIGR00656  70 rerdelvsvGEllssallssalrelgvkaealdgkeagilTddefgnAkikelateerLlelLeegiiv 138
                                               re+d+l+ +GE+++++lls+al+e+g+ a++++g++ +i Td+ +++A+i++++   ++ ++L++g++v
  lcl|NCBI__GCF_000934765.1:WP_044409599.1  64 REMDMLLTTGEQVTISLLSMALQEKGYGAISYTGWQIPIQTDEVHTKARIDSINA-DKIHDQLNQGKVV 131
                                               *******************************************************.9************ PP

                                 TIGR00656 139 vvaGFiGateeGeiTtLGRGGSDltAallaaalkAdrveiyTDVeGvyttDPrvveeakkidkisyeEa 207
                                               vvaGF+G t +G+i tLGRGGSD+tA++laaalkAd+++iyTDV+GvyttDPrvv+ea+++d+i+++E+
  lcl|NCBI__GCF_000934765.1:WP_044409599.1 132 VVAGFQGVTANGDISTLGRGGSDTTAVALAAALKADECQIYTDVDGVYTTDPRVVPEARRLDTITFDEM 200
                                               ********************************************************************* PP

                                 TIGR00656 208 lelAtlGakvlhpralelaveakvpilvrsskekeegTlitn...kkensslvkaialeknvarltveg 273
                                               le+A+lGakvl+ r++e+a ++kvp++v ss  +  gTlit+   ++e++ l+++ia++++ ++l+++ 
  lcl|NCBI__GCF_000934765.1:WP_044409599.1 201 LEMASLGAKVLQIRSVEFASKYKVPLRVLSSLTEGGGTLITSednNMEKP-LISGIAFNRDESKLQIR- 267
                                               *****************************************977666666.*****************. PP

                                 TIGR00656 274 egmlgkrgilaeifkaLaeeeinvdlisqtese...tsislvvdeedvdeakkaLkeesgaaeleslev 339
                                                g+ +k+gi+  + + +a+++i +d+i+q +++   t+ +++v + d  +a+++L+   + ++ +++  
  lcl|NCBI__GCF_000934765.1:WP_044409599.1 268 -GVPDKPGIAFALLGPIADANIEIDMIIQNQGQdgtTDFTFTVPRGDRAHAHEILEGIAQVLGAREVIF 335
                                               .9*****************************99999********************************* PP

                                 TIGR00656 340 eedlavvsivgaglveapGvaseifkaleekninilmisssetkisvlvdekdaekavrklheklee 406
                                               +  +a+vs+vg+g++++ G+as++fk+l+++nini mi++ e+kisv+++e ++e+av++lh+++e+
  lcl|NCBI__GCF_000934765.1:WP_044409599.1 336 DDSIAKVSLVGVGMRSHVGIASRMFKVLADQNINIQMIATTEIKISVVIKESELESAVKSLHQQFEL 402
                                               *****************************************************************97 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (407 nodes)
Target sequences:                          1  (405 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.02
# Mc/sec: 7.26
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis