Alignments for a candidate for asp-kinase in Oleispira antarctica

GapMind for Amino acid biosynthesis

Alignments for a candidate for asp-kinase in Oleispira antarctica

Align aspartate kinase (EC 2.7.2.4) (characterized)
to candidate WP_046009782.1 OLEAN_RS14300 aspartate kinase

Query= BRENDA::O69077
         (412 letters)



>NCBI__GCF_000967895.1:WP_046009782.1
          Length = 407

 Score =  592 bits (1527), Expect = e-174
 Identities = 313/406 (77%), Positives = 344/406 (84%), Gaps = 1/406 (0%)

Query: 1   MALIVQKFGGTSVGTVERIEQVAEKVKKFREAGDDVVVVVSAMSGETNRLIGLANQIMEQ 60
           MAL VQK+GGTSVGTVERIE VA+KVK F +AG  +V+ VSAMSGETNRLIGLAN I   
Sbjct: 1   MALYVQKYGGTSVGTVERIEHVADKVKSFHDAGHQMVIAVSAMSGETNRLIGLANSISSN 60

Query: 61  PVPRELDVMVSTGEQVTIALLSMALIKRGVPAVSYTGNQVRILTDSAHTKARILHIDDTH 120
           P PRE+DV+VSTGEQVTIALL++AL KRGV A S+TG QV I TDSAH KARI  ID   
Sbjct: 61  PTPREMDVLVSTGEQVTIALLAIALQKRGVGAKSFTGWQVAIKTDSAHMKARIEDIDTVA 120

Query: 121 IRADLKAGRVVVVAGFQGVDGNGNITTLGRGGSDTTGVALAAALKADECQIYTDVDGVYT 180
           IR  L AG V +VAGFQGVDG  NITTLGRGGSDTTGVALAAAL ADE QIYTDVDGVYT
Sbjct: 121 IRKQLDAGNVCIVAGFQGVDGENNITTLGRGGSDTTGVALAAALGADEAQIYTDVDGVYT 180

Query: 181 TDPRVVPQARRLDKITFEEMLEMASLGSKVLQIRAVEFAGKYNVPLRVLHSFQEGPGTLI 240
           TDPRVVP ARR++K+TFEEMLEMASLGSK+LQIRAVEFAGKY VPLRVL SF +GPGTLI
Sbjct: 181 TDPRVVPNARRMEKVTFEEMLEMASLGSKILQIRAVEFAGKYKVPLRVLSSFDDGPGTLI 240

Query: 241 TIDDEEESMEQPIISGIAFNRDEAKLTIRGVPDTPGVAFKILGPISAANVEVDMIVQNVA 300
           T+ +E+ S+E P+ISGIAFNRDEAK+T+ GVPD PGVA KIL PIS ANVEVDMIVQNVA
Sbjct: 241 TM-EEDSSVENPVISGIAFNRDEAKVTVTGVPDIPGVASKILCPISDANVEVDMIVQNVA 299

Query: 301 HDNTTDFTFTVHRNDYLNALEILKQTAANIGAREAIGDTNIAKVSIVGVGMRSHAGVASR 360
            D TTDFTFTV RNDY  ALEILK TAA +GAR   G  +I KVS+VGVGMRSHAGVAS+
Sbjct: 300 EDGTTDFTFTVDRNDYAKALEILKNTAAELGARNVSGTDDICKVSMVGVGMRSHAGVASK 359

Query: 361 MFEALAKESINIQMISTSEIKVSVVIEEKYLELAVRALHTAFELDA 406
           MF+ LA ESINIQ I+TSEIK+SVVI EKYLELAVRALHTAF+LDA
Sbjct: 360 MFKVLADESINIQAITTSEIKISVVIAEKYLELAVRALHTAFDLDA 405


Lambda     K      H
   0.317    0.133    0.362 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 537
Number of extensions: 17
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 412
Length of database: 407
Length adjustment: 31
Effective length of query: 381
Effective length of database: 376
Effective search space:   143256
Effective search space used:   143256
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 50 (23.9 bits)

Align candidate WP_046009782.1 OLEAN_RS14300 (aspartate kinase)
to HMM TIGR00656 (aspartate kinase, monofunctional class (EC 2.7.2.4))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00656.hmm
# target sequence database:        /tmp/gapView.8158.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00656  [M=407]
Accession:   TIGR00656
Description: asp_kin_monofn: aspartate kinase, monofunctional class
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   3.3e-131  424.0  12.9   3.7e-131  423.8  12.9    1.0  1  lcl|NCBI__GCF_000967895.1:WP_046009782.1  OLEAN_RS14300 aspartate kinase


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000967895.1:WP_046009782.1  OLEAN_RS14300 aspartate kinase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  423.8  12.9  3.7e-131  3.7e-131       2     406 ..       2     403 ..       1     404 [. 0.98

  Alignments for each domain:
  == domain 1  score: 423.8 bits;  conditional E-value: 3.7e-131
                                 TIGR00656   2 eliVqKFGGtsvgsserikkaakivlkelkegkkvvVVvSAmskvtdelvelaellklleaisdeispr 70 
                                                l VqK+GGtsvg +eri+++a++v++    g+++v+ vSAms++t++l+ la      ++is++++pr
  lcl|NCBI__GCF_000967895.1:WP_046009782.1   2 ALYVQKYGGTSVGTVERIEHVADKVKSFHDAGHQMVIAVSAMSGETNRLIGLA------NSISSNPTPR 64 
                                               688*************************************************9......8********* PP

                                 TIGR00656  71 erdelvsvGEllssallssalrelgvkaealdgkeagilTddefgnAkikelateerLlelLeegiivv 139
                                               e+d lvs+GE++++all+ al+++gv a++++g++ +i Td+ + +A+i++++t   + ++L+ g + +
  lcl|NCBI__GCF_000967895.1:WP_046009782.1  65 EMDVLVSTGEQVTIALLAIALQKRGVGAKSFTGWQVAIKTDSAHMKARIEDIDT-VAIRKQLDAGNVCI 132
                                               ******************************************************.9************* PP

                                 TIGR00656 140 vaGFiGateeGeiTtLGRGGSDltAallaaalkAdrveiyTDVeGvyttDPrvveeakkidkisyeEal 208
                                               vaGF+G + e +iTtLGRGGSD+t ++laaal Ad+ +iyTDV+GvyttDPrvv++a++++k+++eE+l
  lcl|NCBI__GCF_000967895.1:WP_046009782.1 133 VAGFQGVDGENNITTLGRGGSDTTGVALAAALGADEAQIYTDVDGVYTTDPRVVPNARRMEKVTFEEML 201
                                               ********************************************************************* PP

                                 TIGR00656 209 elAtlGakvlhpralelaveakvpilvrsskekeegTlitn....kkensslvkaialeknvarltveg 273
                                               e+A+lG k+l+ ra+e+a ++kvp++v ss++   gTlit     + en +++++ia++++ a++tv+ 
  lcl|NCBI__GCF_000967895.1:WP_046009782.1 202 EMASLGSKILQIRAVEFAGKYKVPLRVLSSFDDGPGTLITMeedsSVEN-PVISGIAFNRDEAKVTVT- 268
                                               ****************************************966633444.6*****************. PP

                                 TIGR00656 274 egmlgkrgilaeifkaLaeeeinvdlisqtese...tsislvvdeedvdeakkaLkeesgaaeleslev 339
                                                g+ + +g++++i   ++++++ vd+i+q  +e   t+ +++vd++d  +a ++Lk++  +++ ++++ 
  lcl|NCBI__GCF_000967895.1:WP_046009782.1 269 -GVPDIPGVASKILCPISDANVEVDMIVQNVAEdgtTDFTFTVDRNDYAKALEILKNTAAELGARNVSG 336
                                               .9*****************************99999********************************* PP

                                 TIGR00656 340 eedlavvsivgaglveapGvaseifkaleekninilmisssetkisvlvdekdaekavrklheklee 406
                                                 d+++vs+vg+g++++ Gvas++fk+l+++ ini  i++se+kisv++ ek++e avr+lh ++ +
  lcl|NCBI__GCF_000967895.1:WP_046009782.1 337 TDDICKVSMVGVGMRSHAGVASKMFKVLADESINIQAITTSEIKISVVIAEKYLELAVRALHTAFDL 403
                                               ***************************************************************9976 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (407 nodes)
Target sequences:                          1  (407 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.02
# Mc/sec: 7.69
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis