Align homoserine kinase (EC 2.7.1.39) (characterized)
to candidate WP_051957978.1 Q366_RS10275 shikimate kinase
Query= metacyc::MONOMER-21144 (185 letters) >NCBI__GCF_000745975.1:WP_051957978.1 Length = 170 Score = 118 bits (295), Expect = 6e-32 Identities = 64/160 (40%), Positives = 90/160 (56%) Query: 21 VSIIGMAGAGKTTVGRELALQLGWAHVDTDNLIEATYGTRLQAVADSMDKESFLDVEAGV 80 V++IGM GK+TVG LA QLG+ +DTD LI+ L + + FLD+EAG Sbjct: 8 VALIGMPAVGKSTVGVLLAKQLGYDFLDTDILIQTKENMTLAQIIGKKGVQGFLDIEAGH 67 Query: 81 IRRIGARRTVLSTGGSVVYRHEAMAHLAALGPLVYLDVSLPLILKRIAMNPDRGLAIAPG 140 + +G +R V+STGGSV+YR +AM HL + ++YL L +L R++ RG+AI P Sbjct: 68 LLGLGGKRRVISTGGSVIYREQAMRHLKKIATVIYLHADLSTLLTRLSDLEARGVAIDPN 127 Query: 141 QTIEDLYNERIALYRRYATFTVAADALSPGGCATRIVAWL 180 I+ LY ER LY ++ +A PG A I L Sbjct: 128 SNIDALYKERTPLYDKFCDIKIACGQKLPGQVAADIAVAL 167 Lambda K H 0.321 0.137 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 100 Number of extensions: 2 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 185 Length of database: 170 Length adjustment: 19 Effective length of query: 166 Effective length of database: 151 Effective search space: 25066 Effective search space used: 25066 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 44 (21.6 bits)
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory