Alignments for a candidate for asd in Nitriliruptor alkaliphilus DSM 45188

GapMind for Amino acid biosynthesis

Alignments for a candidate for asd in Nitriliruptor alkaliphilus DSM 45188

Align aspartate-semialdehyde dehydrogenase (EC 1.2.1.11) (characterized)
to candidate WP_052664753.1 NITAL_RS03505 aspartate-semialdehyde dehydrogenase

Query= BRENDA::P9WNX5
         (345 letters)



>NCBI__GCF_000969705.1:WP_052664753.1
          Length = 352

 Score =  281 bits (719), Expect = 2e-80
 Identities = 171/355 (48%), Positives = 216/355 (60%), Gaps = 17/355 (4%)

Query: 1   MGLSIGIVGATGQVGQVMRTLLDERDFPASAVR-FFASARSQGRKLAFRGQEIEVEDAET 59
           MG+ I +VGATG VG+ +R LL  RD          ASARS G KLA+R  E+EV +   
Sbjct: 1   MGIKIAVVGATGAVGREVRHLLATRDIDIEGDPVLLASARSAGTKLAWRDGEVEVREVSE 60

Query: 60  ADPSGLDIALFSAGSAMSKVQAPRFAAAGVTVIDNSSAWRKDPDVPLVVSEVNFERDAHR 119
              +G+DIA+FSAG A SK   P  AA G  V+DNSSA+R D DVPLVVSEVN E     
Sbjct: 61  DAFAGVDIAIFSAGGARSKEWGPIAAAQGAVVVDNSSAFRMDDDVPLVVSEVNPEA-LRT 119

Query: 120 RPKGIIANPNCTTMAAMPVLKVLHDEARLVRLVVSSYQAVSGSGLAGVAELAEQARAVIG 179
           RPKGI+ANPNCTTM  M  +K LHD   L  +V +SYQA  G+G AG+ EL EQ+  ++G
Sbjct: 120 RPKGIVANPNCTTMVLMVAVKPLHDAFGLTDVVATSYQAAGGAGQAGIRELLEQSAKLVG 179

Query: 180 GAEQLVYDGGALEFP-PPNTYVAPIAFNVVPLAGSLVDDGSGETDEDQKLRFESRKILGI 238
             E+L   G   E      T+   IAFN +P  G+  DD    TDE+ KL  ESRKILG+
Sbjct: 180 DEERLRSRGLEAEAAVEAETFSKAIAFNTLPHCGNFADD--RYTDEEWKLVNESRKILGL 237

Query: 239 PDLLVSGTCVRVPVFTGHSLSINAEFAQPLSPERARELLDGATGVQLVD----------V 288
           P L VS TCVRVPV  GH ++    FA+P++ +     L+GA G+ + D           
Sbjct: 238 PALRVSPTCVRVPVVVGHGIAARLSFAEPVTRDGLMAALEGAPGLVIEDGTGQDGEALAY 297

Query: 289 PTPLAAAGVDESLVGRIRRDPGVPDGRGLALFVSGDNLRKGAALNTIQIAELLTA 343
           P+ L +AG DE +VGR+R D  + D     LFV+GDNL KGAALN +Q+AEL+ A
Sbjct: 298 PSTLGSAGRDEVIVGRVRED--LADPHSANLFVAGDNLLKGAALNAVQLAELIAA 350


Lambda     K      H
   0.317    0.135    0.382 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 359
Number of extensions: 21
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 345
Length of database: 352
Length adjustment: 29
Effective length of query: 316
Effective length of database: 323
Effective search space:   102068
Effective search space used:   102068
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 49 (23.5 bits)

Align candidate WP_052664753.1 NITAL_RS03505 (aspartate-semialdehyde dehydrogenase)
to HMM TIGR01296 (asd: aspartate-semialdehyde dehydrogenase (EC 1.2.1.11))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01296.hmm
# target sequence database:        /tmp/gapView.9865.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01296  [M=339]
Accession:   TIGR01296
Description: asd_B: aspartate-semialdehyde dehydrogenase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   4.3e-117  376.9   0.0   4.8e-117  376.8   0.0    1.0  1  lcl|NCBI__GCF_000969705.1:WP_052664753.1  NITAL_RS03505 aspartate-semialde


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000969705.1:WP_052664753.1  NITAL_RS03505 aspartate-semialdehyde dehydrogenase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  376.8   0.0  4.8e-117  4.8e-117       1     336 [.       4     348 ..       4     351 .. 0.95

  Alignments for each domain:
  == domain 1  score: 376.8 bits;  conditional E-value: 4.8e-117
                                 TIGR01296   1 nvaivGatGavGqellkvLeernfpid.klvllasersaGkkvkfkgkeleveeaekesfegidialfs 68 
                                               ++a+vGatGavG+e++++L+ r+ +i+ + vllas+rsaG+k++ ++ e+ev+e+++  f g+dia+fs
  lcl|NCBI__GCF_000969705.1:WP_052664753.1   4 KIAVVGATGAVGREVRHLLATRDIDIEgDPVLLASARSAGTKLAWRDGEVEVREVSEDAFAGVDIAIFS 72 
                                               69************************846799************************************* PP

                                 TIGR01296  69 aGgsvskefapkaakagviviDntsafrldedvPLvvpevnaeelkeakkkgiianPnCstiqlvvvLk 137
                                               aGg+ ske+ p aa++g++v+Dn+safr+d+dvPLvv+evn e l+ ++ kgi+anPnC+t+ l+v++k
  lcl|NCBI__GCF_000969705.1:WP_052664753.1  73 AGGARSKEWGPIAAAQGAVVVDNSSAFRMDDDVPLVVSEVNPEALRTRP-KGIVANPNCTTMVLMVAVK 140
                                               *********************************************9988.******************* PP

                                 TIGR01296 138 plkdeaklkrvvvstYqavsGaGkkgveeLknqtkavlegkekepeida.....lkakkfakqiafnai 201
                                               pl+d+++l  vv  +Yqa  GaG++g+ eL +q    ++ +e+    ++     ++a++f+k iafn +
  lcl|NCBI__GCF_000969705.1:WP_052664753.1 141 PLHDAFGLTDVVATSYQAAGGAGQAGIRELLEQSAKLVGDEERLRSRGLeaeaaVEAETFSKAIAFNTL 209
                                               *********************************9999888888766554556779************** PP

                                 TIGR01296 202 plidklkedGytkeelkllfetrkilgiedlkvsatcvrvPvftghsesvsiefekelsveevkelLke 270
                                               p+ +++ +d yt+ee kl++e+rkilg + l+vs tcvrvPv++gh ++  + f+++++ + +   L++
  lcl|NCBI__GCF_000969705.1:WP_052664753.1 210 PHCGNFADDRYTDEEWKLVNESRKILGLPALRVSPTCVRVPVVVGHGIAARLSFAEPVTRDGLMAALEG 278
                                               ********************************************************************* PP

                                 TIGR01296 271 apgvvvid....dpsenlyptPleavgkdevfvgrirkDlskekglalfvvaDnlrkGaalnavqiael 335
                                               apg+v+ d    d +  +yp+ l  +g+dev vgr+r+Dl + ++ +lfv++Dnl+kGaalnavq+ael
  lcl|NCBI__GCF_000969705.1:WP_052664753.1 279 APGLVIEDgtgqDGEALAYPSTLGSAGRDEVIVGRVREDLADPHSANLFVAGDNLLKGAALNAVQLAEL 347
                                               *****9983333445679*************************************************98 PP

                                 TIGR01296 336 l 336
                                               +
  lcl|NCBI__GCF_000969705.1:WP_052664753.1 348 I 348
                                               7 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (339 nodes)
Target sequences:                          1  (352 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 9.70
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis