Alignments for a candidate for aroC in Nitriliruptor alkaliphilus DSM 45188

GapMind for Amino acid biosynthesis

Alignments for a candidate for aroC in Nitriliruptor alkaliphilus DSM 45188

Align Chorismate synthase; CS; 5-enolpyruvylshikimate-3-phosphate phospholyase; EC 4.2.3.5 (characterized)
to candidate WP_052669746.1 NITAL_RS16680 chorismate synthase

Query= SwissProt::P9WPY1
         (401 letters)



>NCBI__GCF_000969705.1:WP_052669746.1
          Length = 393

 Score =  412 bits (1059), Expect = e-120
 Identities = 217/394 (55%), Positives = 275/394 (69%), Gaps = 4/394 (1%)

Query: 2   LRWITAGESHGRALVAVVEGMVAGVHVTSADIADQLARRRLGYGRGARMTFERDAVTVLS 61
           +R++TAGESHG ALVA +EG+ AG+ V+S D+  +L+RRRLG+GR  RM FE D V VL 
Sbjct: 1   MRYLTAGESHGPALVATLEGLPAGLQVSSQDLVAELSRRRLGHGRSPRMAFEEDRVEVLG 60

Query: 62  GIRHGSTLGGPIAIEIGNTEWPKWETVMAADPVDPA-ELADVARNAPLTRPRPGHADYAG 120
           G+RHG TLG P+++ I NTEWPKW   M  +P D   E+    R   LTRPRPGHAD   
Sbjct: 61  GVRHGVTLGSPVSVVIHNTEWPKWVDAMDPEPRDDLDEIRATGRGQRLTRPRPGHADLTA 120

Query: 121 MLKYGFDDARPVLERASARETAARVAAGTVARAFLRQALGVEVLSHVISIGASAPYEGPP 180
            LKYG+DD R  LERASARETAARV  GT A+  L + +G+ V+SHV++IG     +G  
Sbjct: 121 ALKYGYDDVRDALERASARETAARVVVGTYAKRLLAE-VGISVVSHVVNIGGEQVPDGAA 179

Query: 181 -PRAEDLPAIDASPVRAYDKAAEADMIAQIEAAKKDGDTLGGVVEAVALGLPVGLGSFTS 239
            P  +DL  +DAS VR +D   +A M+A+I+AA  D DTLGG +E +A GLP GLGS   
Sbjct: 180 RPGPDDLDTVDASSVRCFDPDTDARMVARIDAAHADKDTLGGTIEVLAYGLPPGLGSHVH 239

Query: 240 GDHRLDSQLAAAVMGIQAIKGVEIGDGFQTARRRGSRAHDEMYPGPDGVVRS-TNRAGGL 298
            D +LD++LAAA + +QA+KGVE GDGF  A R GS+AHDE+    DG  R  ++RAGGL
Sbjct: 240 WDRKLDTRLAAACLSVQAMKGVEFGDGFDLASRPGSQAHDEIVREDDGTYRRLSDRAGGL 299

Query: 299 EGGMTNGQPLRVRAAMKPISTVPRALATVDLATGDEAVAIHQRSDVCAVPAAGVVVETMV 358
           E GM+ GQPLR+R AMKPIS++P+ LATVDL T   A AI QRSD CAVP AGVV+E++V
Sbjct: 300 EAGMSTGQPLRLRVAMKPISSLPQPLATVDLDTHQPAQAITQRSDACAVPRAGVVLESVV 359

Query: 359 ALVLARAALEKFGGDSLAETQRNIAAYQRSVADR 392
           A  LA A LEK GGD ++E +RN+  Y  S+  R
Sbjct: 360 AFELADALLEKTGGDQVSEVRRNLDGYLSSLEGR 393


Lambda     K      H
   0.317    0.133    0.380 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 468
Number of extensions: 20
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 401
Length of database: 393
Length adjustment: 31
Effective length of query: 370
Effective length of database: 362
Effective search space:   133940
Effective search space used:   133940
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 50 (23.9 bits)

Align candidate WP_052669746.1 NITAL_RS16680 (chorismate synthase)
to HMM TIGR00033 (aroC: chorismate synthase (EC 4.2.3.5))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00033.hmm
# target sequence database:        /tmp/gapView.28195.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00033  [M=351]
Accession:   TIGR00033
Description: aroC: chorismate synthase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   5.6e-124  399.6   0.0   6.4e-124  399.4   0.0    1.0  1  lcl|NCBI__GCF_000969705.1:WP_052669746.1  NITAL_RS16680 chorismate synthas


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000969705.1:WP_052669746.1  NITAL_RS16680 chorismate synthase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  399.4   0.0  6.4e-124  6.4e-124       1     349 [.       1     372 [.       1     374 [. 0.96

  Alignments for each domain:
  == domain 1  score: 399.4 bits;  conditional E-value: 6.4e-124
                                 TIGR00033   1 lrlttfGeSHgkalgaiidGlPaglelteediqkelkrRrpgqsrltrmrkEeDeveilsGvfeGkTtG 69 
                                               +r++t+GeSHg+al+a+++GlPagl+++ +d+  el+rRr g++r  rm  EeD+ve+l+Gv++G T G
  lcl|NCBI__GCF_000969705.1:WP_052669746.1   1 MRYLTAGESHGPALVATLEGLPAGLQVSSQDLVAELSRRRLGHGRSPRMAFEEDRVEVLGGVRHGVTLG 69 
                                               89******************************************************************* PP

                                 TIGR00033  70 aPiallikNkd.......vrskdyed...........ikelpRPgHadytylkKYgikd.regggrsSa 119
                                               +P++++i+N++       +++++ +d             ++pRPgHad+t+  KYg +d r  ++r+Sa
  lcl|NCBI__GCF_000969705.1:WP_052669746.1  70 SPVSVVIHNTEwpkwvdaMDPEPRDDldeiratgrgqRLTRPRPGHADLTAALKYGYDDvRDALERASA 138
                                               ***************999777777778888888888799****************************** PP

                                 TIGR00033 120 ReTaarvaaGavakklLketagieivayvvklgeveleeesak...eiskerldkspvrcpdaeaekem 185
                                               ReTaarv++G+ ak+lL+e  gi +v++vv++g  ++++  a+   +   +++d+s vrc d++++++m
  lcl|NCBI__GCF_000969705.1:WP_052669746.1 139 RETAARVVVGTYAKRLLAE-VGISVVSHVVNIGGEQVPDGAARpgpD-DLDTVDASSVRCFDPDTDARM 205
                                               *******************.88***************9988887743.6788999************** PP

                                 TIGR00033 186 eeeidkakkdgdsvGgvvevvvsnvpvglG..eplfdkldaelasallsinAvKgveiGdGFeaasvrG 252
                                               ++ id a+ d+d++Gg +ev+++++p glG  +++++kld++la+a ls++A+Kgve+GdGF++as++G
  lcl|NCBI__GCF_000969705.1:WP_052669746.1 206 VARIDAAHADKDTLGGTIEVLAYGLPPGLGshVHWDRKLDTRLAAACLSVQAMKGVEFGDGFDLASRPG 274
                                               ******************************9999*********************************** PP

                                 TIGR00033 253 seanDelvle.ddkirrktnnsGGieGGitnGedirvriavKpiptikkplktvdletkekakatkgRh 320
                                               s+a+De+v e d+ +rr +++ GG+e G+++G+++r+r+a+Kpi+++ +pl+tvdl+t+++a+a ++R+
  lcl|NCBI__GCF_000969705.1:WP_052669746.1 275 SQAHDEIVREdDGTYRRLSDRAGGLEAGMSTGQPLRLRVAMKPISSLPQPLATVDLDTHQPAQAITQRS 343
                                               **********88899****************************************************** PP

                                 TIGR00033 321 DpcvvpravpvvEamvalvladallekra 349
                                               D c+vpra++v E++va+ ladallek++
  lcl|NCBI__GCF_000969705.1:WP_052669746.1 344 DACAVPRAGVVLESVVAFELADALLEKTG 372
                                               **************************986 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (351 nodes)
Target sequences:                          1  (393 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.01
# Mc/sec: 8.04
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis