Alignments for a candidate for ilvE in Pseudarthrobacter sulfonivorans Ar51

GapMind for Amino acid biosynthesis

Alignments for a candidate for ilvE in Pseudarthrobacter sulfonivorans Ar51

Align branched-chain-amino-acid transaminase (EC 2.6.1.42) (characterized)
to candidate WP_058932081.1 AU252_RS19160 branched-chain amino acid aminotransferase

Query= BRENDA::A0R066
         (368 letters)



>NCBI__GCF_001484605.1:WP_058932081.1
          Length = 370

 Score =  427 bits (1098), Expect = e-124
 Identities = 219/366 (59%), Positives = 265/366 (72%), Gaps = 8/366 (2%)

Query: 6   LEFTVSANTNPATDAVRESILANPGFGKYYTDHMVSIDYTVDE----GWHNAQVIPYGPI 61
           +EF    +  P +   R +ILANPGFG Y+TDH   +DY+VDE    GWHNA+V  YGPI
Sbjct: 8   VEFNQQLSATPKSAEERAAILANPGFGNYFTDHTAVVDYSVDEQGEGGWHNARVEAYGPI 67

Query: 62  QLDPSAIVLHYGQEIFEGLKAYRWADGSIVSFRPEANAARLQSSARRLAIPELPEEVFIE 121
            LDPSA VLHYGQEIFEGLKAYR ADGSI +FRPEANAARL  SARRLA+PELPEE F+ 
Sbjct: 68  SLDPSAAVLHYGQEIFEGLKAYRHADGSIWTFRPEANAARLNKSARRLALPELPEEYFLG 127

Query: 122 SLRQLIAVDEKWVPPAGGEESLYLRPFVIATEPGLGVRPSNEYRYLLIASPAGAYFKGGI 181
           ++R+L+  D++WVP AG  E+LYLRPF+IATE  LGVR + E  + +IASPAG YF G +
Sbjct: 128 AIRELVQADKEWVP-AGDGEALYLRPFMIATEAFLGVRAAREVSFRVIASPAGNYFGGEL 186

Query: 182 KPVSVWLSHEYVRASPGGTGAAKFGGNYAASLLAQAQAAEMGCDQVVWLDAIERRYVEEM 241
           KPVS+W+S EY RA  GGTGAAK GGNYAASL+AQ +A   GC QV++LD      VEE+
Sbjct: 187 KPVSIWISREYARAGRGGTGAAKCGGNYAASLIAQQEAEANGCKQVLFLDHFNDNAVEEL 246

Query: 242 GGMNLFFVFGSGGSARLVTPELSGSLLPGITRDSLLQLATDAGFAVEERKIDVDEWQKKA 301
           GGMN+FFV   G    LVTP LSG++L G+TR S++Q+A D G  V ERKI +DEW+   
Sbjct: 247 GGMNVFFVMKDGS---LVTPALSGTILEGVTRMSVIQVAKDMGREVAERKITLDEWRDGV 303

Query: 302 GAGEITEVFACGTAAVITPVSHVKHHDGEFTIADGQPGEITMALRDTLTGIQRGTFADTH 361
            +GEI EVFACGTAAVITP+  +K         D + GE TMA+R+ L GIQ GT  DTH
Sbjct: 304 ASGEIAEVFACGTAAVITPIGVLKDTTEFIGSEDAKAGETTMAIRERLLGIQTGTVPDTH 363

Query: 362 GWMARL 367
           GW+ RL
Sbjct: 364 GWLTRL 369


Lambda     K      H
   0.318    0.135    0.409 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 466
Number of extensions: 18
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 368
Length of database: 370
Length adjustment: 30
Effective length of query: 338
Effective length of database: 340
Effective search space:   114920
Effective search space used:   114920
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 49 (23.5 bits)

Align candidate WP_058932081.1 AU252_RS19160 (branched-chain amino acid aminotransferase)
to HMM TIGR01123 (ilvE: branched-chain amino acid aminotransferase (EC 2.6.1.42))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01123.hmm
# target sequence database:        /tmp/gapView.1249.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01123  [M=313]
Accession:   TIGR01123
Description: ilvE_II: branched-chain amino acid aminotransferase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   1.8e-137  443.5   0.0   2.7e-137  442.9   0.0    1.2  1  lcl|NCBI__GCF_001484605.1:WP_058932081.1  AU252_RS19160 branched-chain ami


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_001484605.1:WP_058932081.1  AU252_RS19160 branched-chain amino acid aminotransferase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  442.9   0.0  2.7e-137  2.7e-137       1     312 [.      56     368 ..      56     369 .. 0.99

  Alignments for each domain:
  == domain 1  score: 442.9 bits;  conditional E-value: 2.7e-137
                                 TIGR01123   1 WdeaelaseaeleldegsavlhYgqevfeGlkayRtadGkillfRpdanakRlrrsaerlllPeleeel 69 
                                               W++a+++++++++ld+++avlhYgqe+feGlkayR+adG+i++fRp+ana+Rl++sa+rl+lPel+ee 
  lcl|NCBI__GCF_001484605.1:WP_058932081.1  56 WHNARVEAYGPISLDPSAAVLHYGQEIFEGLKAYRHADGSIWTFRPEANAARLNKSARRLALPELPEEY 124
                                               ********************************************************************* PP

                                 TIGR01123  70 flealkqlvkadkdwvpkakseasLYlRPfliatednlGvkaakeylflvlasPvGaYfkgglapvsif 138
                                               fl a+++lv+adk+wvp  + +++LYlRPf+iate+ lGv+aa+e+ f v+asP+G+Yf g+l+pvsi+
  lcl|NCBI__GCF_001484605.1:WP_058932081.1 125 FLGAIRELVQADKEWVPAGD-GEALYLRPFMIATEAFLGVRAAREVSFRVIASPAGNYFGGELKPVSIW 192
                                               *****************665.9*********************************************** PP

                                 TIGR01123 139 veteyvRaapkGtGavkvgGnYaasllaqkkaaeqglddvvyldpvekkkieevGaaniflitkdgelv 207
                                               ++ ey+Ra  +GtGa+k+gGnYaasl aq++a+++g+++v++ld  +++ +ee+G++n+f+++kdg+lv
  lcl|NCBI__GCF_001484605.1:WP_058932081.1 193 ISREYARAGRGGTGAAKCGGNYAASLIAQQEAEANGCKQVLFLDHFNDNAVEELGGMNVFFVMKDGSLV 261
                                               ********************************************************************* PP

                                 TIGR01123 208 ttplsesiLegvtresllelakdlgleveereiaidelkaaveaGei..vfacGtaavitPvgelkieg 274
                                               t++ls+ iLegvtr s++++akd+g ev er+i++de+++ v++Gei  vfacGtaavitP+g lk   
  lcl|NCBI__GCF_001484605.1:WP_058932081.1 262 TPALSGTILEGVTRMSVIQVAKDMGREVAERKITLDEWRDGVASGEIaeVFACGTAAVITPIGVLKDTT 330
                                               **********************************************999******************** PP

                                 TIGR01123 275 kevevkseevGevtkklrdeltdiqyGkledkegWive 312
                                               +++  ++++ Ge t+++r+ l++iq+G++ d++gW+++
  lcl|NCBI__GCF_001484605.1:WP_058932081.1 331 EFIGSEDAKAGETTMAIRERLLGIQTGTVPDTHGWLTR 368
                                               ***********************************987 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (313 nodes)
Target sequences:                          1  (370 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.01
# Mc/sec: 9.60
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis