Align Bifunctional aspartokinase/homoserine dehydrogenase 2, chloroplastic; AK-HD 2; AK-HSDH 2; Beta-aspartyl phosphate homoserine 2; EC 2.7.2.4; EC 1.1.1.3 (characterized)
to candidate WP_066920368.1 ACG33_RS08530 bifunctional aspartate kinase/homoserine dehydrogenase I
Query= SwissProt::O81852 (916 letters) >NCBI__GCF_001579945.1:WP_066920368.1 Length = 826 Score = 741 bits (1912), Expect = 0.0 Identities = 387/831 (46%), Positives = 538/831 (64%), Gaps = 14/831 (1%) Query: 87 EMWSVHKFGGTCVGNSQRIRNVAEVIINDNSERKLVVVSAMSKVTDMMYDLIRKAQSRDD 146 E W VHKFGG+ V ++ + VA ++ D R +V+SA VTD + ++ A+ R+D Sbjct: 5 ERWRVHKFGGSSVADAVCMERVARILEQDPHPRMGIVLSACRGVTDTLLGVVAAAERRED 64 Query: 147 SYLSALEAVLEKHRLTARDLLDGDDLASFLSHLHNDISNLKAMLRAIYIAGHASESFSDF 206 +E + +H A LL + F+S L D ++ ++L I + +S+ D Sbjct: 65 -VTGRIEELRRRHETIADTLLPEEARQEFMSLLTQDCRDIASILHTISLIRFSSQMVRDL 123 Query: 207 VAGHGELWSAQMLSYVVRKTGL---ECKWMDTRDVLIVNPTSSNQVDPDFGESEKRLDKW 263 VAG+GE+WS ++ S +R G + +W+D R+V++V S + ES++ +D+ Sbjct: 124 VAGYGEIWSTRLFSAYLRHRGRRPGQVQWVDAREVVVVEWGSLGP-GVRWTESQENMDRL 182 Query: 264 FSLNPSKIIIATGFIASTPQNIPTTLKRDGSDFSAAIMGALLRARQVTIWTDVDGVYSAD 323 P+ +II TGFIA P+ + TTL R+GSDFS +I GALL A Q+ IWTDVDGV SAD Sbjct: 183 VPSGPATLII-TGFIARDPKGLQTTLGRNGSDFSGSIFGALLNAEQIHIWTDVDGVLSAD 241 Query: 324 PRKVNEAVILQTLSYQEAWEMSYFGANVLHPRTIIPVMRYNIPIVIRNIFNLSAPGTIIC 383 PR V EA ++ +LSY EA E++YFGA V+HP+T+ P +R IPI IRN F PGT+IC Sbjct: 242 PRLVPEAKVIDSLSYSEAMELAYFGAKVIHPQTMAPAVRKGIPIWIRNSFAPEKPGTLIC 301 Query: 384 QPPEDDYDLKLTTPVKGFATIDNLALINVEGTGMAGVPGTASDIFGCVKDVGANVIMISQ 443 P VKG ID +AL+N+EG GM GVPGTA +FG ++D G +VI+ISQ Sbjct: 302 ANPSSQL------AVKGITCIDRVALVNLEGAGMIGVPGTAHRLFGALRDHGISVILISQ 355 Query: 444 ASSEHSVCFAVPEKEVNAVSEALRSRFSEALQAGRLSQIEVIPNCSILAAVGQKMASTPG 503 SSEHS+CFA+PE E +R F LQ G + +EV NCSILA VG MA + G Sbjct: 356 GSSEHSICFAIPEAESVRAETVVRQAFERELQDGHIQSVEVEDNCSILAVVGDGMAGSHG 415 Query: 504 VSCTLFSALAKANINVRAISQGCSEYNVTVVIKREDSVKALRAVHSRFFLSRTTLAMGIV 563 + +F AL A ++VRAI+QG SE N++ VI + S +ALR+VHS F+LS T+++G++ Sbjct: 416 AAAKVFGALGAAAVSVRAIAQGASERNISAVIDGKHSARALRSVHSSFYLSPHTISIGLI 475 Query: 564 GPGLIGATLLDQLRDQAAVLKQEFNIDLRVLGITGSKKMLLSDIGIDLSRWRELLNEKGT 623 GPGL+G LLDQ Q L +E +DLRV GI GS +M L+ I L RWR+ Sbjct: 476 GPGLVGGVLLDQFASQVERLSRELKVDLRVRGIMGSSRMHLAGHEIPLGRWRDAFGAHQE 535 Query: 624 EADLDKFTQQVHGNHFIPNSVVVDCTADSAIASRYYDWLRKGIHVITPNKKANSGPLDQY 683 AD+ +F Q V+ +H +P+++++DCT+ +A Y WL GIHV+TPNKKANS L Y Sbjct: 536 PADMARFVQHVNADH-LPHAIIIDCTSSLEVAQHYPAWLAGGIHVVTPNKKANSADLPFY 594 Query: 684 LKLRDLQRKSYTHYFYEATVGAGLPIISTLRGLLETGDKILRIEGICSGTLSYLFNNFVG 743 L+L++ R+ THY YEATVGAGLPI+ TLR L TGD+I RIEGI SGTLSYLFN + G Sbjct: 595 LRLQEASRQGNTHYMYEATVGAGLPIVQTLRDLHGTGDRIQRIEGILSGTLSYLFNVWDG 654 Query: 744 DRSFSEVVTEAKNAGFTEPDPRDDLSGTDVARKVIILARESGLKLDLADLPIRSLVPEPL 803 + FS+V+ A+ G+TEPDPRDDLSG D ARK+IILARE GL+L++ D+ + LVPE L Sbjct: 655 SQPFSQVLHTARAKGYTEPDPRDDLSGIDFARKIIILAREMGLRLEMQDVQLEGLVPEAL 714 Query: 804 KGCTSVEEFMEKLPQYDGDLAKERLDAENSGEVLRYVGVVDAVNQKGTVELRRYKKEHPF 863 C + +EF+++LP++D +A+ A+ G VLRYVG +DA + +V L ++ H F Sbjct: 715 TSC-APQEFLDRLPEFDAPIAERLRAAQARGRVLRYVGAIDAASSSASVGLVELERSHTF 773 Query: 864 AQLAGSDNIIAFTTTRYKDHPLIVRGPGAGAQVTAGGIFSDILRLASYLGA 914 A + +DN++ F T RY +PLIV+GPGAG VTAGG+F+D+LR+ +YLGA Sbjct: 774 ANINLTDNVVRFVTQRYNQNPLIVQGPGAGPDVTAGGVFADLLRVCAYLGA 824 Lambda K H 0.319 0.135 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1712 Number of extensions: 72 Number of successful extensions: 8 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 916 Length of database: 826 Length adjustment: 42 Effective length of query: 874 Effective length of database: 784 Effective search space: 685216 Effective search space used: 685216 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 56 (26.2 bits)
Align candidate WP_066920368.1 ACG33_RS08530 (bifunctional aspartate kinase/homoserine dehydrogenase I)
to HMM TIGR00657 (aspartate kinase (EC 2.7.2.4))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00657.hmm # target sequence database: /tmp/gapView.26911.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00657 [M=442] Accession: TIGR00657 Description: asp_kinases: aspartate kinase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 5e-113 364.4 0.0 6.1e-113 364.1 0.0 1.1 1 lcl|NCBI__GCF_001579945.1:WP_066920368.1 ACG33_RS08530 bifunctional aspar Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_001579945.1:WP_066920368.1 ACG33_RS08530 bifunctional aspartate kinase/homoserine dehydrogenase I # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 364.1 0.0 6.1e-113 6.1e-113 5 440 .. 9 463 .. 5 465 .. 0.95 Alignments for each domain: == domain 1 score: 364.1 bits; conditional E-value: 6.1e-113 TIGR00657 5 VqKFGGtSvgnverikkvakivkkekekgnqvvVVvSAmagvTdaLvelaekvsseee.keliekirek 72 V KFGG+Sv+++ +++va+i++++ + + + +V+SA gvTd L+ ++ ++++e+ + ie++r++ lcl|NCBI__GCF_001579945.1:WP_066920368.1 9 VHKFGGSSVADAVCMERVARILEQDPHPR--MGIVLSACRGVTDTLLGVVAAAERREDvTGRIEELRRR 75 99*********************999666..889******************9999988889******* PP TIGR00657 73 hlealeelasqalkeklkallekeleevkk............ereldlilsvGEklSaallaaaleelg 129 h++++++l +++ ++++ +ll + + + + +dl+ +GE S++l++a+l+++g lcl|NCBI__GCF_001579945.1:WP_066920368.1 76 HETIADTLLPEEARQEFMSLLTQDCRDIASilhtislirfssQMVRDLVAGYGEIWSTRLFSAYLRHRG 144 *********888888888888888888888899*******99999************************ PP TIGR00657 130 v..kavsllgaeagiltd.sefgrAkvleeikterleklleeg.iivvvaGFiGatekgeittLGRGGS 194 +v++++a+++++ + +++g + ++++e+++ l+ g +++GFi+ ++kg ttLGR+GS lcl|NCBI__GCF_001579945.1:WP_066920368.1 145 RrpGQVQWVDAREVVVVEwGSLGPGV-RWTESQENMDRLVPSGpATLIITGFIARDPKGLQTTLGRNGS 212 98779999999998887725666666.9999999999999999999*********************** PP TIGR00657 195 DltAallAaalkAdeveiytDVdGiytaDPrivpeArrldeisyeEalELaslGakvLhprtlepamra 263 D++ ++ +a+l+A++++i+tDVdG+++aDPr+vpeA+ +d++sy Ea+ELa++Gakv+hp+t+ pa+r+ lcl|NCBI__GCF_001579945.1:WP_066920368.1 213 DFSGSIFGALLNAEQIHIWTDVDGVLSADPRLVPEAKVIDSLSYSEAMELAYFGAKVIHPQTMAPAVRK 281 ********************************************************************* PP TIGR00657 264 kipivvkstfnpeaeGTlivakskseeepavkalsldknqalvsvsgttmk..pgilaevfgalaeakv 330 +ipi ++++f pe++GTli+a+ ++++ avk+++ + alv+++g++m pg++ ++fgal+++++ lcl|NCBI__GCF_001579945.1:WP_066920368.1 282 GIPIWIRNSFAPEKPGTLICAN--PSSQLAVKGITCIDRVALVNLEGAGMIgvPGTAHRLFGALRDHGI 348 **********************..55779**************************************** PP TIGR00657 331 nvdlilqsssetsisfvvdkedadkakellk...kkvkeekaleevevekklalvslvGagmksapgva 396 +v+li+q sse+si f++++ + +a+++ + + ++++ +++veve++ ++ ++vG+gm++ g a lcl|NCBI__GCF_001579945.1:WP_066920368.1 349 SVILISQGSSEHSICFAIPEAESVRAETVVRqafERELQDGHIQSVEVEDNCSILAVVGDGMAGSHGAA 417 **********************99999988767777889999*************************** PP TIGR00657 397 akifeaLaeeniniemis..sseikisvvvdekdaekavealhekl 440 ak+f aL + +++++i+ +se++is v+d+k + +a++ +h+ + lcl|NCBI__GCF_001579945.1:WP_066920368.1 418 AKVFGALGAAAVSVRAIAqgASERNISAVIDGKHSARALRSVHSSF 463 ******************9************************998 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (442 nodes) Target sequences: 1 (826 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.03u 0.01s 00:00:00.04 Elapsed: 00:00:00.03 # Mc/sec: 10.99 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory