Alignments for a candidate for hisN in Steroidobacter denitrificans DSM 18526

GapMind for Amino acid biosynthesis

Alignments for a candidate for hisN in Steroidobacter denitrificans DSM 18526

Align Histidinol-phosphatase [alternative form] (EC 3.1.3.15) (characterized)
to candidate WP_066921539.1 ACG33_RS12155 histidinol-phosphatase

Query= reanno::Phaeo:GFF2154
         (250 letters)



>NCBI__GCF_001579945.1:WP_066921539.1
          Length = 281

 Score =  212 bits (540), Expect = 6e-60
 Identities = 115/244 (47%), Positives = 150/244 (61%), Gaps = 1/244 (0%)

Query: 1   MADAARQAILPYFRSAGLQSDNKLDEGFDPVTIADRAAEQAMRSVLSELRPEDSILGEEF 60
           +AD A   ILP+FR+  L  +NK  +GFDPVT+ADR AE A+R  +    P   ILGEE 
Sbjct: 31  LADLAGAQILPHFRTRLLDVENKDRQGFDPVTLADREAEAAIRREIQRTYPTHGILGEEH 90

Query: 61  GETHGQSGRTWVLDPIDGTRGFISGTPTWGVLIALGDADGPFLGIVDQPYIGERFIGTPE 120
           G   G S  TWV+DPIDGTR F+ G   WG LIAL D   P LG++ QPY GE F+G+ +
Sbjct: 91  GLQVGASPYTWVIDPIDGTRAFVLGQLHWGTLIALNDGTRPVLGVMRQPYTGETFLGSEQ 150

Query: 121 GASLTGPLGHSALVTRATDSLSEATLFTTFPEV-GTEAERAAFQRVSAQVRLTRYGMDCY 179
           GA L      + L  RA   L EA L  T P +      R AF+RV+A+ R  R+G DCY
Sbjct: 151 GAQLRRGSQTTRLSARAGVRLEEAVLCATDPLMFAAPGLRPAFERVAARARAVRFGGDCY 210

Query: 180 AYALLAAGQCDLVIEAGLNAYDIQAPIAVIQAAGGVVTNWQGEPAHEGGQVLAAATAELH 239
              L+AAG  DLV+EA L  +D+QA I +I+ AGG++T+W G+ A E  +V+ A+ A LH
Sbjct: 211 TPCLVAAGSSDLVVEADLKPWDVQALIPIIEGAGGIITDWAGQAAWEADKVVIASGAGLH 270

Query: 240 AAAL 243
           A  +
Sbjct: 271 AQVI 274


Lambda     K      H
   0.317    0.134    0.392 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 224
Number of extensions: 11
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 250
Length of database: 281
Length adjustment: 25
Effective length of query: 225
Effective length of database: 256
Effective search space:    57600
Effective search space used:    57600
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 47 (22.7 bits)

Align candidate WP_066921539.1 ACG33_RS12155 (histidinol-phosphatase)
to HMM TIGR02067 (hisN: histidinol-phosphatase (EC 3.1.3.15))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR02067.hmm
# target sequence database:        /tmp/gapView.5565.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR02067  [M=252]
Accession:   TIGR02067
Description: his_9_HisN: histidinol-phosphatase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
    1.5e-88  282.5   0.0    1.7e-88  282.3   0.0    1.0  1  lcl|NCBI__GCF_001579945.1:WP_066921539.1  ACG33_RS12155 histidinol-phospha


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_001579945.1:WP_066921539.1  ACG33_RS12155 histidinol-phosphatase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  282.3   0.0   1.7e-88   1.7e-88       2     252 .]      25     278 ..      24     278 .. 0.98

  Alignments for each domain:
  == domain 1  score: 282.3 bits;  conditional E-value: 1.7e-88
                                 TIGR02067   2 lalalelaeaageailkyfrasdlkvdkksdk..tpVteADraaEeaireliaakfPddgilGEEfgee 68 
                                               l++a +la++ag++il++fr++ l v++k+++  +pVt ADr+aE+air+ i++++P +gilGEE+g +
  lcl|NCBI__GCF_001579945.1:WP_066921539.1  25 LEFAVHLADLAGAQILPHFRTRLLDVENKDRQgfDPVTLADREAEAAIRREIQRTYPTHGILGEEHGLQ 93 
                                               6899*************************9999************************************ PP

                                 TIGR02067  69 eedaeyvWvlDPiDGTksFirGvPvwgtLiaLlekgkpvlGvisqPalgerfvaakgegallngge.re 136
                                                +++ y+Wv+DPiDGT++F+ G   wgtLiaL+++ +pvlGv++qP++ge f+++++++ l++g++  +
  lcl|NCBI__GCF_001579945.1:WP_066921539.1  94 VGASPYTWVIDPIDGTRAFVLGQLHWGTLIALNDGTRPVLGVMRQPYTGETFLGSEQGAQLRRGSQtTR 162
                                               ********************************************************99999998556** PP

                                 TIGR02067 137 lrvsevaklsdAvlvttspkaledeeereafeklrrkarltryggdcyayalvAsGrvdlvveaelspy 205
                                               l++++  +l++Avl+ t+p ++++++ r afe+++++ar +r+ggdcy  +lvA+G+ dlvvea+l+p+
  lcl|NCBI__GCF_001579945.1:WP_066921539.1 163 LSARAGVRLEEAVLCATDPLMFAAPGLRPAFERVAARARAVRFGGDCYTPCLVAAGSSDLVVEADLKPW 231
                                               ********************************************************************* PP

                                 TIGR02067 206 DiaalipiieeAggvitdwkGkeaeeggeavaaanaalhdevlellk 252
                                               D++alipiie+Agg+itdw+G+ a e  ++v+a+ a lh++v+e+l+
  lcl|NCBI__GCF_001579945.1:WP_066921539.1 232 DVQALIPIIEGAGGIITDWAGQAAWEADKVVIASGAGLHAQVIEALN 278
                                               ********************************************985 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (252 nodes)
Target sequences:                          1  (281 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.01
# Mc/sec: 6.66
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis