Align diaminopimelate decarboxylase (EC 4.1.1.20) (characterized)
to candidate WP_068461975.1 APY04_RS09415 diaminopimelate decarboxylase
Query= reanno::Korea:Ga0059261_2051 (419 letters) >NCBI__GCF_001541235.1:WP_068461975.1 Length = 422 Score = 415 bits (1067), Expect = e-120 Identities = 218/422 (51%), Positives = 283/422 (67%), Gaps = 5/422 (1%) Query: 1 MDHFNRKNGVLHAENVSIPAIAAEVGTPVYVYSTATLERHASALKNALAGLPSVHLAFAI 60 M HF ++GVLHAE VS+ IA EVGTP Y YSTATLERH L A AG ++ + FA+ Sbjct: 1 MHHFEYRDGVLHAEGVSLARIAEEVGTPFYCYSTATLERHYRVLTEAFAGQDAL-ICFAV 59 Query: 61 KANPNLAVLGVLARQGYGADVVSGGELKRALAAGMPAEDVVFSGVGKTRAELQLGLDEGI 120 KAN N AVL LAR G G DVVS GEL+RALA G+PA ++F+GVGKTR+E+ L+ GI Sbjct: 60 KANSNQAVLATLARLGAGMDVVSEGELRRALAVGVPASRIIFAGVGKTRSEMAFALEAGI 119 Query: 121 GQFNLELEEEGEVLADLAHAQGKTAPAVLRVNPDVDAGTHAKISTGKAENKFGVAIDRAL 180 FN+E E E L+++A A GKTA LRVNPDVDA THAKISTGKAENKFG+ +A Sbjct: 120 LGFNVESEPELRALSEVASALGKTAHVALRVNPDVDAKTHAKISTGKAENKFGIPYAQAR 179 Query: 181 EIFDRLAKRPGLNLRGVAIHIGSQLTELAPLEAAYKRVGELVAQLRAAGHTITHVDLGGG 240 +++ A PG+ G+ +HIGSQ+T+L P A++ + E+V QL A GH + H+D+GGG Sbjct: 180 QLYAEAASLPGIAATGIHMHIGSQITQLDPFRNAFRLMREMVEQLIADGHKLQHLDIGGG 239 Query: 241 LGVPY-HAGQTVSTAEEFGAMVARVTQGWNVTLMFEPGRFICGNAGVLVTEVIWVKPAAG 299 LGVPY G+ + +V G + ++ EPGR I GNAGVLVT+V++ K Sbjct: 240 LGVPYVSEGEVTPPPSAYAQIVREELAGLGLKIVMEPGRVIAGNAGVLVTQVVYCKEGVD 299 Query: 300 NPYVIVDAAMNDLARPALYDAYHEFEAVEP---TGEKFVANIAGPVCETGDTFAMGREID 356 + +VDAAMNDL RP LY+A HE VE + V +I GPVCETGD A+ R++ Sbjct: 300 KTFTVVDAAMNDLIRPTLYEALHEIWPVEEDKLSMPPVVQDIVGPVCETGDYLALDRKLP 359 Query: 357 VVKSGDLAVFRTAGAYGATMASTYNSRALVPEVLVSGDRFAVVADRIQPETIMGAERVPE 416 + SGDL TAGAYGA M+S+YN+R LVPEVLV GD +AVV R E ++G +++P+ Sbjct: 360 PLSSGDLIAVMTAGAYGAVMSSSYNTRPLVPEVLVHGDAYAVVRPRFGIEQLIGLDKLPD 419 Query: 417 WV 418 W+ Sbjct: 420 WL 421 Lambda K H 0.318 0.135 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 534 Number of extensions: 27 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 419 Length of database: 422 Length adjustment: 32 Effective length of query: 387 Effective length of database: 390 Effective search space: 150930 Effective search space used: 150930 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 50 (23.9 bits)
Align candidate WP_068461975.1 APY04_RS09415 (diaminopimelate decarboxylase)
to HMM TIGR01048 (lysA: diaminopimelate decarboxylase (EC 4.1.1.20))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR01048.hmm # target sequence database: /tmp/gapView.15776.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01048 [M=417] Accession: TIGR01048 Description: lysA: diaminopimelate decarboxylase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 2.6e-148 479.9 0.0 3e-148 479.7 0.0 1.0 1 lcl|NCBI__GCF_001541235.1:WP_068461975.1 APY04_RS09415 diaminopimelate de Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_001541235.1:WP_068461975.1 APY04_RS09415 diaminopimelate decarboxylase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 479.7 0.0 3e-148 3e-148 4 415 .. 6 414 .. 3 416 .. 0.97 Alignments for each domain: == domain 1 score: 479.7 bits; conditional E-value: 3e-148 TIGR01048 4 kkdgeleiegvdlkelaeefgtPlYvydeetlrerlealkeafkaeeslvlYAvKAnsnlavlrllaee 72 +dg l +egv+l+++aee gtP+Y y+++tl+++++ l+eaf+++++l+++AvKAnsn avl +la++ lcl|NCBI__GCF_001541235.1:WP_068461975.1 6 YRDGVLHAEGVSLARIAEEVGTPFYCYSTATLERHYRVLTEAFAGQDALICFAVKANSNQAVLATLARL 74 57899*****************************************9********************** PP TIGR01048 73 GlgldvvsgGEleralaAgvkaekivfsgngkseeeleaaleleiklinvdsveelelleeiakelgkk 141 G+g+dvvs+GEl rala gv+a++i+f+g+gk+++e++ ale++i +nv+s+ el+ l+e+a lgk+ lcl|NCBI__GCF_001541235.1:WP_068461975.1 75 GAGMDVVSEGELRRALAVGVPASRIIFAGVGKTRSEMAFALEAGILGFNVESEPELRALSEVASALGKT 143 ********************************************************************* PP TIGR01048 142 arvllRvnpdvdaktheyisTGlkesKFGieveeaeeayelalkleslelvGihvHIGSqildlepfve 210 a+v+lRvnpdvdakth +isTG++e+KFGi +++a++ y a++l+ + +Gih+HIGSqi++l+pf++ lcl|NCBI__GCF_001541235.1:WP_068461975.1 144 AHVALRVNPDVDAKTHAKISTGKAENKFGIPYAQARQLYAEAASLPGIAATGIHMHIGSQITQLDPFRN 212 ********************************************************************* PP TIGR01048 211 aaekvvklleelkeegieleeldlGGGlgisyeeeee.apdleeyaeklleklekeaelglklklilEp 278 a++ + +++e+l ++g +l++ld+GGGlg++y +e e +p ++ ya+ + e+l++ l lk+++Ep lcl|NCBI__GCF_001541235.1:WP_068461975.1 213 AFRLMREMVEQLIADGHKLQHLDIGGGLGVPYVSEGEvTPPPSAYAQIVREELAG-----LGLKIVMEP 276 ********************************88765268889999999999988.....89******* PP TIGR01048 279 GRslvanagvlltrVesvKevesrkfvlvDagmndliRpalYeayheiaalkr.leeeetetvdvvGpl 346 GR +++nagvl+t+V + Ke ++f +vDa+mndliRp lYea hei ++++ + ++ d+vGp+ lcl|NCBI__GCF_001541235.1:WP_068461975.1 277 GRVIAGNAGVLVTQVVYCKEGVDKTFTVVDAAMNDLIRPTLYEALHEIWPVEEdKLSMPPVVQDIVGPV 345 ***************************************************88466788999******* PP TIGR01048 347 CEsgDvlakdrelpeveeGdllavasaGAYgasmssnYnsrprpaevlveegkarlirrretledllal 415 CE+gD la dr+lp +Gdl+av+ aGAYga mss Yn+rp + evlv+++ + ++r r +e+l+ l lcl|NCBI__GCF_001541235.1:WP_068461975.1 346 CETGDYLALDRKLPPLSSGDLIAVMTAGAYGAVMSSSYNTRPLVPEVLVHGDAYAVVRPRFGIEQLIGL 414 ************************************************************999999865 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (417 nodes) Target sequences: 1 (422 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.02 # Mc/sec: 8.50 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory