Align acetolactate synthase (subunit 1/2) (EC 2.2.1.6) (characterized)
to candidate WP_068462596.1 APY04_RS10530 acetolactate synthase small subunit
Query= BRENDA::P00894 (163 letters) >NCBI__GCF_001541235.1:WP_068462596.1 Length = 182 Score = 124 bits (311), Expect = 8e-34 Identities = 69/158 (43%), Positives = 104/158 (65%), Gaps = 3/158 (1%) Query: 3 RILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTD-DPTLSRMTIQTVGDEKVLEQIE 61 R LS++++NE G L+RVIGLFS RGYNI+SLTV T+ + LSR+TI T+G V+ QI+ Sbjct: 22 RTLSIIVDNEPGVLARVIGLFSGRGYNIDSLTVTETEHEKHLSRITIVTLGTPDVIVQIK 81 Query: 62 KQLHKLVDVLRVSEL-GQGAHVEREIMLVKIQASGYGRDEVKRNTEIFRGQIIDVTPSLY 120 QL +LV V RV +L +G +ERE+ LVK+ G R E R EIFR +++D + + Sbjct: 82 HQLERLVPVHRVRDLTAEGVALERELALVKVVGKGDKRIEALRIAEIFRAKVVDTSTEHF 141 Query: 121 TVQLAGTSGKLDAFLASIRDVAKIVEVARSGVVGLSRG 158 ++ G KL+ F+ + ++ +VEV+R+G+ + RG Sbjct: 142 VFEITGKPSKLEEFIQIMTELG-LVEVSRTGIAAIGRG 178 Lambda K H 0.318 0.136 0.360 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 81 Number of extensions: 5 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 163 Length of database: 182 Length adjustment: 18 Effective length of query: 145 Effective length of database: 164 Effective search space: 23780 Effective search space used: 23780 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 44 (21.6 bits)
Align candidate WP_068462596.1 APY04_RS10530 (acetolactate synthase small subunit)
to HMM TIGR00119 (ilvN: acetolactate synthase, small subunit (EC 2.2.1.6))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00119.hmm # target sequence database: /tmp/gapView.27913.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00119 [M=158] Accession: TIGR00119 Description: acolac_sm: acetolactate synthase, small subunit Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 4.5e-54 168.8 1.9 5.2e-54 168.6 1.9 1.0 1 lcl|NCBI__GCF_001541235.1:WP_068462596.1 APY04_RS10530 acetolactate synth Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_001541235.1:WP_068462596.1 APY04_RS10530 acetolactate synthase small subunit # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 168.6 1.9 5.2e-54 5.2e-54 3 158 .] 22 179 .. 20 179 .. 0.96 Alignments for each domain: == domain 1 score: 168.6 bits; conditional E-value: 5.2e-54 TIGR00119 3 hvlsvlvenepGvLsrvsGlfarrgfniesltvgetee.kdlsrmtivvegddkvveqiekqleklvdv 70 ++ls++v+nepGvL+rv+Glf+ rg+ni+sltv+ete+ k+lsr+tiv+ g +v+ qi++qle+lv+v lcl|NCBI__GCF_001541235.1:WP_068462596.1 22 RTLSIIVDNEPGVLARVIGLFSGRGYNIDSLTVTETEHeKHLSRITIVTLGTPDVIVQIKHQLERLVPV 90 89**********************************96368**************************** PP TIGR00119 71 lkvldltes.eivkrelvlvkvsalgeerneikelteifrgrvvDvsedslivelsgkedkisaflkll 138 +v+dlt + ++rel+lvkv +g +r e +++eifr++vvD s + ++ e++gk+ k++ f++++ lcl|NCBI__GCF_001541235.1:WP_068462596.1 91 HRVRDLTAEgVALERELALVKVVGKGDKRIEALRIAEIFRAKVVDTSTEHFVFEITGKPSKLEEFIQIM 159 ******9651679******************************************************** PP TIGR00119 139 kefgikevarsGlvalsrge 158 e+g++ev+r+G+ a+ rg+ lcl|NCBI__GCF_001541235.1:WP_068462596.1 160 TELGLVEVSRTGIAAIGRGP 179 ****************9985 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (158 nodes) Target sequences: 1 (182 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00 # Mc/sec: 5.06 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory