Align D-3-phosphoglycerate dehydrogenase; EC 1.1.1.95 (characterized, see rationale)
to candidate WP_073040765.1 BUB04_RS14555 phosphoglycerate dehydrogenase
Query= uniprot:Q6LWW6 (523 letters) >NCBI__GCF_900129305.1:WP_073040765.1 Length = 526 Score = 406 bits (1043), Expect = e-117 Identities = 219/524 (41%), Positives = 333/524 (63%), Gaps = 6/524 (1%) Query: 3 KILITDPLHESAVEILKQAGEVEVATGLTV--EELKLKIKDVDALVIRSGTTATREIIEA 60 KILI D +H +++L+ ++EV EE++ D DALV+RS T T ++I A Sbjct: 2 KILICDGMHPKGLDLLRAEDDLEVLAPDQPGPEEVRELAADCDALVVRSRTKVTEDLISA 61 Query: 61 SENLKVIARAGVGVDNVDLDAATEKGIVVVNAPDASSISVAELLFGMMLAAARNIPQATA 120 + LKVI RAG GVDN+D+ AA+ +GI+V+N P A++++ AE +MLA AR++PQAT Sbjct: 62 APRLKVIGRAGTGVDNIDIAAASARGILVMNTPGANAMAAAEHTMALMLALARHVPQATQ 121 Query: 121 SIKSGKWDRKSFKGMEIYGKTLGIVGLGRIGQQVAKRAQAFGMTIVAYDPYIPEDVASEL 180 S++ G+W++K F G E+Y +TLGI+GLG+IG VA RA M ++ YDP+I + A+ + Sbjct: 122 SLRDGRWEKKRFMGTELYQQTLGIIGLGKIGAIVADRALGMKMNVLVYDPHITPETAAIM 181 Query: 181 GIKLLTVDELCTVSDFITLHVPLTPKTKHMIGKEQIALMKSNMVIMNCARGGLIDEAALY 240 G + + ++EL SDFITLH PLTP T+++I ++ IALMK + I+NCARGGL+DE ALY Sbjct: 182 GTEWVPLEELFQRSDFITLHTPLTPDTRNIINRDSIALMKKGVRIINCARGGLVDEEALY 241 Query: 241 DALNSGKIKAAALDVFEQEPPKESPLLTLNNLIGTPHQGASTEEAQLSAGTIVAEQTVKI 300 +AL SG + AALDVF +EPP+ +PLL L N+I TPH GAS+ +AQ + +A Q V Sbjct: 242 EALVSGHVAGAALDVFAKEPPEGNPLLRLENVIFTPHLGASSFQAQENVARAIASQIVDY 301 Query: 301 LKGESAENVVNLPMVPTEKMKKLKPYMVLAEKMGSMAIQYLDNSIELLEITYMG-GLAKE 359 LK N VN P + +++KPY+ LAE++G + Q L + IE +EI Y G L + Sbjct: 302 LKRGIIRNAVNFPSISPRDYERIKPYLTLAERLGHLQGQ-LCSPIERVEIEYSGPELTEL 360 Query: 360 KTEILKRSFLKGILAPILLAGVNLVNAPVIAKSRNIKIAEGTMSES-DYGNSIKISAKGE 418 + L +S KG+L PIL VNLVNAP++ K R I++ T SE+ Y I+++ +G+ Sbjct: 361 PLDPLTQSIAKGLLDPILAEKVNLVNAPMLLKERKIQLVATTTSEARGYTGLIRVTVEGK 420 Query: 419 NDEISIIGSI-EHNEVVFREINGYRMDIKPEGTICIIKHIDRPGMVGKVGVLLGEHGINI 477 + S G++ NE+ +N YR++ + EG I++++D+PG++G +G LG H +NI Sbjct: 421 GRKRSATGAVFPGNEIRLVRMNTYRLEAELEGINLIVQNVDKPGVIGFIGTTLGTHQVNI 480 Query: 478 AGMQVGRREPGGHSIMFLDIDHMISDEVLDEIRKMENVRAAKSI 521 A M + R +I + +D E LD +R ++ + + + Sbjct: 481 ANMHLSRTPEKDRAIAIIRLDGEAPPEALDALRSNPDILSVEQV 524 Lambda K H 0.316 0.134 0.364 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 634 Number of extensions: 27 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 523 Length of database: 526 Length adjustment: 35 Effective length of query: 488 Effective length of database: 491 Effective search space: 239608 Effective search space used: 239608 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 52 (24.6 bits)
Align candidate WP_073040765.1 BUB04_RS14555 (phosphoglycerate dehydrogenase)
to HMM TIGR01327 (serA: phosphoglycerate dehydrogenase (EC 1.1.1.95))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR01327.hmm # target sequence database: /tmp/gapView.8735.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01327 [M=525] Accession: TIGR01327 Description: PGDH: phosphoglycerate dehydrogenase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 8.8e-199 647.5 0.0 9.8e-199 647.3 0.0 1.0 1 lcl|NCBI__GCF_900129305.1:WP_073040765.1 BUB04_RS14555 phosphoglycerate d Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_900129305.1:WP_073040765.1 BUB04_RS14555 phosphoglycerate dehydrogenase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 647.3 0.0 9.8e-199 9.8e-199 1 523 [. 2 524 .. 2 526 .] 0.98 Alignments for each domain: == domain 1 score: 647.3 bits; conditional E-value: 9.8e-199 TIGR01327 1 kvlvadklseegie.llkekelevdvktglskeellekikdydalivRSatkvteelleaaekLkvigR 68 k+l++d ++++g++ l +e++lev ++ ee+ e d dal+vRS+tkvte+l++aa++LkvigR lcl|NCBI__GCF_900129305.1:WP_073040765.1 2 KILICDGMHPKGLDlLRAEDDLEVLAPDQPGPEEVRELAADCDALVVRSRTKVTEDLISAAPRLKVIGR 70 79************7778899************************************************ PP TIGR01327 69 aGvGvDNidieaatkkGilvvNaPegntisaaElalalllalaRkipqadksvkekkWerkkflGtEly 137 aG GvDNidi aa+++Gilv+N+P +n ++aaE+++al+lalaR++pqa++s+++++We+k+f+GtEly lcl|NCBI__GCF_900129305.1:WP_073040765.1 71 AGTGVDNIDIAAASARGILVMNTPGANAMAAAEHTMALMLALARHVPQATQSLRDGRWEKKRFMGTELY 139 ********************************************************************* PP TIGR01327 138 gktlGviGlGriGsevakrakalgmkvlayDPyiseekaeklgvellkdldellaeaDvitvHvPltke 206 ++tlG+iGlG+iG +va+ra +++m+vl+yDP+i++e a+ +g e l+el++++D+it+H+Plt++ lcl|NCBI__GCF_900129305.1:WP_073040765.1 140 QQTLGIIGLGKIGAIVADRALGMKMNVLVYDPHITPETAAIMGTE-WVPLEELFQRSDFITLHTPLTPD 207 *********************************************.555******************** PP TIGR01327 207 tkgligkeelakmKkgviivNcaRGGiidEkALvealeegkvraaalDvfekEPptdnklleldnvvvt 275 t+++i+++++a mKkgv+i+NcaRGG++dE+AL+eal +g+v++aalDvf+kEPp+ n+ll+l+nv+ t lcl|NCBI__GCF_900129305.1:WP_073040765.1 208 TRNIINRDSIALMKKGVRIINCARGGLVDEEALYEALVSGHVAGAALDVFAKEPPEGNPLLRLENVIFT 276 ********************************************************************* PP TIGR01327 276 pHlgAsteEaqenvavevaeevlealkgevvesavNlpaldaeeleklkpyldlaeklGklasqllkea 344 pHlgAs aqenva +a++++++lk + ++avN p+++ + e++kpyl+lae+lG+l +ql + lcl|NCBI__GCF_900129305.1:WP_073040765.1 277 PHLGASSFQAQENVARAIASQIVDYLKRGIIRNAVNFPSISPRDYERIKPYLTLAERLGHLQGQLCS-P 344 ****************************************************************986.6 PP TIGR01327 345 vkkvevtleG.elaeeeselltsallkgllkevleeevnlvnAkavakergitveeskeeesedyknll 412 +++ve++++G el+e ++lt+++ kgll+++l+e+vnlvnA+++ ker+i++ ++++e++ y+ l+ lcl|NCBI__GCF_900129305.1:WP_073040765.1 345 IERVEIEYSGpELTELPLDPLTQSIAKGLLDPILAEKVNLVNAPMLLKERKIQLVATTTSEARGYTGLI 413 778*******89********************************************************* PP TIGR01327 413 evkveadkgevsvagtvleekepriveidgfevdlepegilliiknkDkpGvigkvgsllgeagiNias 481 +v+ve++ ++s +g+v+ ++e r+v++++++++ e egi+li++n DkpGvig +g++lg++++Nia+ lcl|NCBI__GCF_900129305.1:WP_073040765.1 414 RVTVEGKGRKRSATGAVFPGNEIRLVRMNTYRLEAELEGINLIVQNVDKPGVIGFIGTTLGTHQVNIAN 482 ********************************************************************* PP TIGR01327 482 mqlgrkekggealmllslDeevseevleeikevpeiksvklv 523 m+l+r+ +++ a+++++lD e+++e l+++++ p+i sv+ v lcl|NCBI__GCF_900129305.1:WP_073040765.1 483 MHLSRTPEKDRAIAIIRLDGEAPPEALDALRSNPDILSVEQV 524 ***************************************988 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (525 nodes) Target sequences: 1 (526 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.03 # Mc/sec: 7.86 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory