Alignments for a candidate for leuA in Alkalihalobacterium alkalinitrilicum DSM 22532

GapMind for Amino acid biosynthesis

Alignments for a candidate for leuA in Alkalihalobacterium alkalinitrilicum DSM 22532

Align 2-isopropylmalate synthase (EC 2.3.3.13) (characterized)
to candidate WP_078428591.1 BK574_RS10690 citramalate synthase

Query= BRENDA::Q58595
         (518 letters)



>NCBI__GCF_002019605.1:WP_078428591.1
          Length = 542

 Score =  244 bits (624), Expect = 4e-69
 Identities = 166/522 (31%), Positives = 284/522 (54%), Gaps = 31/522 (5%)

Query: 23  RVYIFDTTLRDGEQTPGVSLTPEEKIDIAIKLDDLGVDVIEAGFPVSSLGEQEAIKKICS 82
           +V+++DTTLRDG Q  GVSL+ E+K+ IA KLD+LG+  IE G+P S+  +     K+  
Sbjct: 5   KVFLYDTTLRDGTQGEGVSLSVEDKLKIAKKLDELGIHYIEGGWPGSNPKDMNFFGKVKD 64

Query: 83  LNL---------DAEICGLARAVKKDIDVAIDCGVDRIHTFIATSPLHRKYKLKKSKEEI 133
           L L              G+     +++   ++ GV  +  F  T      + L+ + +E 
Sbjct: 65  LKLKNATVTAFGSTRRMGVTPDKDQNLQRILESGVKSVAIFGKTWDFQVTHALQTTLDEN 124

Query: 134 IDIAVDAIEYIKEHGIRVEFSAE---DATRTEIDYLIEVYKKAVDAGADIINVPDTVGVM 190
           + +  D+++Y+KE+G+ V F AE   D  +   +Y I+  KKA +AGAD + + DT G  
Sbjct: 125 LSMIYDSVKYLKENGLEVIFDAEHFFDGYKRNAEYAIKAIKKAEEAGADCVTLCDTNGGS 184

Query: 191 IPRAMYYLINELKKEIKVPISVHCHNDFGLAVANSLAAVEAGAEQVHCTINGLGERGGNA 250
           +P  +  ++  + +E+ + + +HCHND  LAVAN+L AV++GA QV  TING GER GN 
Sbjct: 185 LPDEVKSIVQHVVQELSIQVGIHCHNDGELAVANTLVAVQSGATQVQGTINGYGERCGNV 244

Query: 251 ALEEVVMSLMSIYGVKTNIKTQKLYEISQLVSKYT-EIKVQ---PNKAIVGENAFAHESG 306
            L  ++ +L    G +  I++Q+L  ++  VSKY  EI  Q    N+  VG++AFAH+ G
Sbjct: 245 NLISLIPNLQLKMGYEC-IESQELTNLTS-VSKYVHEIANQVPPSNQPFVGKSAFAHKGG 302

Query: 307 IHAHGVLAHALTYEPIPPELVGQKRKIILGKHTGTHAIEAKLKELGIEVGKDINKDQFDE 366
           +H   VL H  TYE I PE +G KR++++ + +G   +  K KE  +++ K  N     E
Sbjct: 303 MHVSAVLKHPETYEHIEPEAIGNKRRVLVSELSGQSNVLFKAKEWNLDLDK--NNPATKE 360

Query: 367 IVKRIKALGDKGKR--VTDRDVEAIVEDVVGKLAKKDRVVELEQIAVMTGNRVIPT-ASV 423
           I+++IK L   G +    +   E +V+  +G+  +  ++   + +    GN+   T A V
Sbjct: 361 IIEQIKELELNGYQYEAAEASFELVVKKGLGEHREFFKLDHFKILIENDGNQSFTTEAVV 420

Query: 424 ALKIEEEIKKSSAIGVGPVDAAVKAIQKAIGE------KIKLKEYHINAI--TGGTDALA 475
            L + ++   ++A G GPV+A   A++KA+ +      ++ L +Y +  +  T  T +  
Sbjct: 421 KLVVNDQEVLTAAEGNGPVNALDHALRKALEQFYPCINQMYLSDYKVRVLDETDATASRV 480

Query: 476 EVIVTLEGYGREITTKAASEDIVRASVEAVIDGINKILAKRE 517
            V++       + +T   S +I++AS  A+ID +   L K++
Sbjct: 481 RVLIESSDGKEKWSTIGVSGNIIKASWYALIDSVQYYLLKQD 522


Lambda     K      H
   0.316    0.135    0.368 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 619
Number of extensions: 26
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 518
Length of database: 542
Length adjustment: 35
Effective length of query: 483
Effective length of database: 507
Effective search space:   244881
Effective search space used:   244881
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis