Align isopropylmalate/citramalate isomerase large subunit LeuC (EC 4.2.1.33; EC 4.2.1.35) (characterized)
to candidate WP_078716441.1 B5D49_RS04295 3-isopropylmalate dehydratase large subunit
Query= reanno::DvH:208495 (419 letters) >NCBI__GCF_900167125.1:WP_078716441.1 Length = 418 Score = 630 bits (1625), Expect = 0.0 Identities = 315/417 (75%), Positives = 350/417 (83%), Gaps = 1/417 (0%) Query: 1 MAHTLAQKILQRHTDEAITDAGQIVRCRVSMVLANDITAPLAIKSFRAMGAKRVFDKDRV 60 M TLA+KI+Q+HT E +AG IVRCRV MVLANDITAPLAIKSF+AMGA RVFD+D+V Sbjct: 1 MPKTLAEKIIQQHTQEP-AEAGNIVRCRVDMVLANDITAPLAIKSFKAMGADRVFDRDKV 59 Query: 61 ALVMDHFTPQKDIEAAQQVKLTREFAREMGVTHYYEGGDCGVEHALLPELGLVGPGDVVV 120 ALV DHFTP KDI++A+QVK REFA MGVTHYYEGG+ GVEHALLPELG+VGPGDVVV Sbjct: 60 ALVCDHFTPNKDIDSAEQVKTVREFAEAMGVTHYYEGGNVGVEHALLPELGIVGPGDVVV 119 Query: 121 GADSHTCTYGGLGAFATGLGSTDVAGAMALGETWFKVPPTIRATFTGTLPAYVGAKDLIL 180 GADSHTCTYGGLGAFATG+GSTD+A AMALGETWFKVPPTIR +GT +VGAKD +L Sbjct: 120 GADSHTCTYGGLGAFATGMGSTDIAAAMALGETWFKVPPTIRVEISGTPQPHVGAKDYML 179 Query: 181 TLIGAIGVDGALYRALEFDGAAIEALDVEGRMTMANMAIEAGGKAGLFAADAKTLTYCTT 240 LIG IGV GALY+ALEF G A+EAL VEGRMTMANMAIEAGGKAGLFA D KT+ Y Sbjct: 180 RLIGEIGVAGALYKALEFGGPAVEALSVEGRMTMANMAIEAGGKAGLFAVDDKTIAYARR 239 Query: 241 AGRTGDTAFSADAGAVYERELSFDVTGMTPVVACPHLPDNVKPVSEVKDVTVQQVVIGSC 300 AGR+G+ A + D+ A YER + + GM+P +ACPHLPDNV V EV + V Q VIGSC Sbjct: 240 AGRSGNQAMTPDSDAGYERVVVIRLDGMSPQIACPHLPDNVHSVDEVAGLRVDQAVIGSC 299 Query: 301 TNGRIGDLREAAAVLRGRKVSRDVRCIVLPATPGIWRQALREGLIETFMEAGCIVGPATC 360 TNGRI D+REAAAVL+G+KVS VRCIVLPATP IW+QAL+EGLIE FMEAGC+VGPATC Sbjct: 300 TNGRIEDMREAAAVLKGKKVSSRVRCIVLPATPAIWKQALKEGLIEVFMEAGCVVGPATC 359 Query: 361 GPCLGGHMGILADGERAIATTNRNFKGRMGSLESEVYLSGPATAAASAVTGVITDPS 417 GPCLGGHMGILA GERAIATTNRNFKGRMGSLESEVYLS PA AAASAV G I PS Sbjct: 360 GPCLGGHMGILAGGERAIATTNRNFKGRMGSLESEVYLSNPAVAAASAVVGEIVHPS 416 Lambda K H 0.320 0.136 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 653 Number of extensions: 22 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 419 Length of database: 418 Length adjustment: 32 Effective length of query: 387 Effective length of database: 386 Effective search space: 149382 Effective search space used: 149382 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 50 (23.9 bits)
Align candidate WP_078716441.1 B5D49_RS04295 (3-isopropylmalate dehydratase large subunit)
to HMM TIGR02083 (leuC: 3-isopropylmalate dehydratase, large subunit (EC 4.2.1.33))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR02083.hmm # target sequence database: /tmp/gapView.14829.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR02083 [M=419] Accession: TIGR02083 Description: LEU2: 3-isopropylmalate dehydratase, large subunit Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 5.5e-193 627.5 1.6 6.4e-193 627.3 1.6 1.0 1 lcl|NCBI__GCF_900167125.1:WP_078716441.1 B5D49_RS04295 3-isopropylmalate Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_900167125.1:WP_078716441.1 B5D49_RS04295 3-isopropylmalate dehydratase large subunit # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 627.3 1.6 6.4e-193 6.4e-193 2 419 .] 4 418 .] 3 418 .] 0.99 Alignments for each domain: == domain 1 score: 627.3 bits; conditional E-value: 6.4e-193 TIGR02083 2 tlaekiladkagkeevkpgelilakldlvlgndvttplaikafkelgvkkvfdkdkvalvldhftpnkd 70 tlaeki+ +++ e ++g+++ ++d+vl+nd+t+plaik+fk +g+++vfd+dkvalv dhftpnkd lcl|NCBI__GCF_900167125.1:WP_078716441.1 4 TLAEKIIQQHTQ-EPAEAGNIVRCRVDMVLANDITAPLAIKSFKAMGADRVFDRDKVALVCDHFTPNKD 71 89*****99975.569***************************************************** PP TIGR02083 71 ikaaeqvklirefakekeiekyfeigelgvehallpekglvvsgdliigadshtctygalgafatgvgs 139 i +aeqvk +refa ++ +y+e g++gvehallpe g+v +gd+++gadshtctyg lgafatg+gs lcl|NCBI__GCF_900167125.1:WP_078716441.1 72 IDSAEQVKTVREFAEAMGVTHYYEGGNVGVEHALLPELGIVGPGDVVVGADSHTCTYGGLGAFATGMGS 140 ********************************************************************* PP TIGR02083 140 tdlavamatgkawfkvpeaikfvlkgklkdyvsakdlilkiigkigvdgalykslefsgeglkelsvdd 208 td+a+ama g++wfkvp +i++ + g +++v akd +l++ig+igv galyk+lef+g++++ lsv++ lcl|NCBI__GCF_900167125.1:WP_078716441.1 141 TDIAAAMALGETWFKVPPTIRVEISGTPQPHVGAKDYMLRLIGEIGVAGALYKALEFGGPAVEALSVEG 209 ********************************************************************* PP TIGR02083 209 rltianmaieagaktgifevdektieyvkgrakrelkiykadedakyervieidlselepqvafphlpe 277 r+t+anmaieag+k+g+f vd+kti+y++ ++ + d da yerv+ i l+ + pq+a phlp+ lcl|NCBI__GCF_900167125.1:WP_078716441.1 210 RMTMANMAIEAGGKAGLFAVDDKTIAYARRAGRSGNQAMTPDSDAGYERVVVIRLDGMSPQIACPHLPD 278 *****************************9999999********************************* PP TIGR02083 278 ntkeideaekeeikidqvvigsctngrledlrlaaeilkgkkvakevrliilpasqkvylealkeglle 346 n +++de++ +++dq+vigsctngr+ed+r aa +lkgkkv+ vr+i+lpa++ ++++alkegl+e lcl|NCBI__GCF_900167125.1:WP_078716441.1 279 NVHSVDEVA--GLRVDQAVIGSCTNGRIEDMREAAAVLKGKKVSSRVRCIVLPATPAIWKQALKEGLIE 345 ********9..99******************************************************** PP TIGR02083 347 ifieagavvstptcgpclgghmgilaegeravsttnrnfvgrmghpksevylaspavaaasaikgkias 415 +f+eag+vv++ tcgpclgghmgila gera++ttnrnf grmg +sevyl+ pavaaasa+ g+i++ lcl|NCBI__GCF_900167125.1:WP_078716441.1 346 VFMEAGCVVGPATCGPCLGGHMGILAGGERAIATTNRNFKGRMGSLESEVYLSNPAVAAASAVVGEIVH 414 ********************************************************************* PP TIGR02083 416 peev 419 p ++ lcl|NCBI__GCF_900167125.1:WP_078716441.1 415 PSHI 418 9875 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (419 nodes) Target sequences: 1 (418 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01 # Mc/sec: 11.90 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory