Align O-acetylhomoserine sulfhydrylase (EC:2.5.1.49) (characterized)
to candidate WP_081423433.1 MCA_RS12205 O-succinylhomoserine sulfhydrylase
Query= reanno::Korea:Ga0059261_3194 (402 letters) >NCBI__GCF_000008325.1:WP_081423433.1 Length = 411 Score = 349 bits (896), Expect = e-101 Identities = 177/377 (46%), Positives = 244/377 (64%), Gaps = 2/377 (0%) Query: 19 TQAIRGGTARSEWGETSEALFLTSGYAYDCAGDAAARFSGDQQGMTYSRLQNPTVEMLEQ 78 T+A+R G R+ E +E +F TS Y + A +AA RF+G G YSR NPTV E+ Sbjct: 31 TRAVRAGQRRTMEQEHAEPIFATSSYVFASAAEAAERFAGKAAGNIYSRFTNPTVRTFEE 90 Query: 79 RIALLEGAEACRATASGMAAMTAALLCQLSAGDHLIGGRAAFGSCRWLTDTQLPKFGIET 138 R+A LEG E C A SGMAA+ + L AGDH++ R+ FG+ L L KFG+ T Sbjct: 91 RLAALEGGERCVAVGSGMAAIASTAFGLLKAGDHVVCSRSVFGNTTLLFQNYLAKFGVPT 150 Query: 139 TVVDARDPQQFIDAIRPNTKVFFFETPANPTMDVVDLKAVCAIARERGIVTVVDNAFATP 198 T V D + AIRP T+ F ETP+NP ++ D+ + IA RG + VVDN F TP Sbjct: 151 TFVGLTDYDGWAAAIRPETRFLFIETPSNPLTEIADIPRLAEIAHSRGCLLVVDNCFCTP 210 Query: 199 ALQRPMDFGADVVAYSATKMMDGQGRVLAGAVCGTEEFINNTLLPFHRNTGPTLSPFNAW 258 ALQRP+ GAD+V +SATK +DGQGR + GA+ G E ++ + PF R GP++SPFNAW Sbjct: 211 ALQRPLALGADIVIHSATKYLDGQGRCVGGAIVGGRELLDAEIYPFLRTGGPSMSPFNAW 270 Query: 259 VVLKGLETLDLRIQRQSENALKVARFLEGR--VPRVNFPGLPSHPQHNLAMSQMAAAGPI 316 V LKGLETL+LR++ ENAL +AR+LE + V RV++PGL SHPQH LA Q + G I Sbjct: 271 VFLKGLETLNLRMKAHCENALGLARWLEAQPWVERVHYPGLASHPQHELAARQQSGFGGI 330 Query: 317 FSIELDGGRTQAHGLLDALGLIDISNNIGDSRSLMTHPASTTHSGVAEDQRLLMGVGEGM 376 S E+ GG+ A L+D+ L+ I+ N+GD+++ +THPA+TTH ++ + R G+ +G+ Sbjct: 331 VSFEVKGGQEAAWRLIDSTRLLSITGNLGDAKTTITHPATTTHGRLSPEARAAAGIADGL 390 Query: 377 LRLNVGLEDPEDLIADL 393 +R+ VGLE+ D+ ADL Sbjct: 391 IRIAVGLENLADIQADL 407 Lambda K H 0.319 0.134 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 449 Number of extensions: 17 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 402 Length of database: 411 Length adjustment: 31 Effective length of query: 371 Effective length of database: 380 Effective search space: 140980 Effective search space used: 140980 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 50 (23.9 bits)
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory