Alignments for a candidate for PPYAT in Bradyrhizobium sp. BTAi1

GapMind for Amino acid biosynthesis

Alignments for a candidate for PPYAT in Bradyrhizobium sp. BTAi1

Align aspartate transaminase (EC 2.6.1.1) (characterized)
to candidate WP_083780753.1 BBTA_RS02905 aspartate/tyrosine/aromatic aminotransferase

Query= BRENDA::W0PFQ7
         (399 letters)



>NCBI__GCF_000015165.1:WP_083780753.1
          Length = 399

 Score =  355 bits (910), Expect = e-102
 Identities = 175/387 (45%), Positives = 248/387 (64%)

Query: 12  PRDPILGLNEQYNADTRDTKVNLGVGVYYDDNGKIPLLKAVQEAERQRVEAHAARGYLPI 71
           P D ++     +  D R  KVNLG+G+YYD+ G+IP L AV+EA+ +    +    YLP 
Sbjct: 9   PPDAVMLAARLFAEDPRPHKVNLGIGMYYDEEGRIPQLAAVREADHRLRSRNRPWPYLPA 68

Query: 72  EGIGNYNKGAQELLFGKDSDVITQGRALTFQALGGTGALKIGADFLKQLQPDSTVYISDP 131
           EG+ +    A  ++FG+D     + R    Q +GGTGA++IGA+  + + PD+   ISDP
Sbjct: 69  EGLVDLKNKAMPVVFGEDQADDLRRRTAWIQTVGGTGAVRIGAELARAIAPDAMASISDP 128

Query: 132 SWENHRALFERAGFKVETYSYYDAATHGLNFDGFAASVKAMPEGSIIVLHACCHNPTGVD 191
           SW NH A+F   G +V +Y YYD  +  ++ DG    +  +P G+++VLH CCHNPTG D
Sbjct: 129 SWPNHEAIFRAVGARVSSYRYYDVESCNIDVDGMLQDLGRLPRGTVVVLHGCCHNPTGFD 188

Query: 192 PSPEQWQQIATLVKERNLVPFLDIAYQGFGAGLQEDAAVVRLFADLGMSMFISSSFSKSF 251
           P+P QW  IA ++ +R L+PF+D+AYQGFG GL+ DA  VR+ A     +F+S SFSKSF
Sbjct: 189 PTPAQWNHIAQVLADRGLIPFIDLAYQGFGEGLEADAQSVRIIAQSCNLIFVSVSFSKSF 248

Query: 252 SLYGERVGALTVVTSSTDEASRVLSQIKRVIRTNYSNPPTHGGMVVAQILNTPELFAQWE 311
           SLYGERVG L VVT S  +A+ V  + + V R  YS+ P++G +++A++L  P+L   W 
Sbjct: 249 SLYGERVGLLFVVTESEAQAALVGERARAVSRALYSSAPSNGALLIAEVLGDPQLKTTWI 308

Query: 312 SELAQMRDRIREMRKQLTDKLNAAGVKQDFNFVMAQRGMFSYSGLTKEQVERLRTEHGIY 371
            EL  MR RI  MR +L D+L       +F  + AQRG+FSYSGLT+ ++ERLR +H ++
Sbjct: 309 DELDAMRRRILLMRAELVDQLAGGNRGANFAPIKAQRGLFSYSGLTRTEIERLRVDHAVH 368

Query: 372 AVNSGRICVAALNSRNIDSVVKAIAAV 398
           AV  GRIC+AALN+ N+ SV  AI  V
Sbjct: 369 AVADGRICLAALNAGNLPSVAAAIRQV 395


Lambda     K      H
   0.318    0.134    0.385 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 391
Number of extensions: 15
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 399
Length of database: 399
Length adjustment: 31
Effective length of query: 368
Effective length of database: 368
Effective search space:   135424
Effective search space used:   135424
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis