Alignments for a candidate for thrC in Nitratiruptor tergarcus DSM 16512

GapMind for Amino acid biosynthesis

Alignments for a candidate for thrC in Nitratiruptor tergarcus DSM 16512

Align Threonine synthase; TS; EC 4.2.3.1 (uncharacterized)
to candidate WP_084276090.1 B8779_RS08115 threonine synthase

Query= curated2:O24924
         (486 letters)



>NCBI__GCF_900176045.1:WP_084276090.1
          Length = 488

 Score =  419 bits (1076), Expect = e-121
 Identities = 232/489 (47%), Positives = 305/489 (62%), Gaps = 8/489 (1%)

Query: 1   MPFVPTRS---LKEKKIDFIEAILNPNAPKGGLYTLERFETLQ---WQDCLNLSYNDLVE 54
           M F+ TR     K KK+ F EAILNP+A  GGLY  E+   +     Q  L   Y +L  
Sbjct: 1   MYFIETRGNDGKKPKKVTFSEAILNPSASYGGLYVPEKLPNIDEKFLQRHLTSHYKELAF 60

Query: 55  CVFERLGLEIPKNLLASALKRYENFDNPKNPAPIFALNERLFVQELYHGPSLAFKDMALQ 114
            +     ++I + L+  AL  Y+ FD+P NP P+  + E LF+ ELYHGP+ AFKDMALQ
Sbjct: 61  DLLRLFSIDIDEELIKEALNLYDKFDDPANPIPVVKVEENLFIAELYHGPTRAFKDMALQ 120

Query: 115 PLASLFSNLAVGKNEKYLMLVSTSGDTGPATLESLAGMPNVFVVCLYPKDGTSLVQKLQM 174
           P   + S+LA  + E YL+L +TSGDTGPATLE+     NV V C+YP  GTS VQ+LQM
Sbjct: 121 PFGYILSSLAKARGENYLILAATSGDTGPATLETFKNRENVKVACIYPAGGTSDVQRLQM 180

Query: 175 VTQSASNLKVFGISGDFDDAQNALKNLLKDDDFNEALKACQLKLSVANSVNFGRIAFQIV 234
           VT+   NLKV GI GDFDDAQ+ALK+LL  + F+  L+   +KLS ANSVNFGRI FQI+
Sbjct: 181 VTEDGENLKVIGIEGDFDDAQSALKSLLASEKFHAILQEKGIKLSAANSVNFGRIIFQII 240

Query: 235 YHIWGFLELYKKGAINSKEKITLAIPSGNFGNALGAFYAKKMGLNIDKIKVVTNSNDVLR 294
           YHI  +LEL ++  I   + I L +PSGNFGNALG +YAKKMGL I KI + +N+N+VL 
Sbjct: 241 YHIHSYLELVRRKEITFGDTINLIVPSGNFGNALGGYYAKKMGLPIKKILIASNANNVLT 300

Query: 295 EFIETGRYDLTHRSLKQTYSPAMDILKSSNVERALFSLFGFERTLELMQALEEEKFYALK 354
           E I  GRYDL ++ L +T SPAMDILKSSNVER LF  FG +RT ELM +L ++ +Y L 
Sbjct: 301 ELINEGRYDLRNKHLIKTSSPAMDILKSSNVERVLFDKFGAQRTKELMDSLNDKGYYKLN 360

Query: 355 PKELALLQEHFSCASCSDEACLKTIQEVYAEHQYLIDPHTATALNASLK-THEKTLVSAT 413
             ELA LQE F+    +D+     I+E   E  YL+DPHTAT +    +   +K +  +T
Sbjct: 361 EDELAALQEDFAADFSNDDEVKAIIKEYALEKTYLMDPHTATTIKLFKRYAADKNVAYST 420

Query: 414 ASYEKFPRITLLALNEQKKNDNDKAALETLKNSYNTPDSQRLDDLFERGIKHQEVLKLNE 473
           A + KF    L AL   +K  +D  AL+ +         QR+ +LF + I H  V+   +
Sbjct: 421 AEWTKFAPTVLEALEGGEKK-SDLEALQIISEKLGVAIPQRIAELFHKRIVHTTVVPKEK 479

Query: 474 IKSSILLWL 482
           I+  IL +L
Sbjct: 480 IEEEILKFL 488


Lambda     K      H
   0.318    0.134    0.381 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 655
Number of extensions: 25
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 486
Length of database: 488
Length adjustment: 34
Effective length of query: 452
Effective length of database: 454
Effective search space:   205208
Effective search space used:   205208
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

Align candidate WP_084276090.1 B8779_RS08115 (threonine synthase)
to HMM TIGR00260 (thrC: threonine synthase (EC 4.2.3.1))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00260.hmm
# target sequence database:        /tmp/gapView.43612.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00260  [M=340]
Accession:   TIGR00260
Description: thrC: threonine synthase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                             Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                             -----------
    6.8e-88  281.0   0.1    9.3e-88  280.6   0.1    1.1  1  NCBI__GCF_900176045.1:WP_084276090.1  


Domain annotation for each sequence (and alignments):
>> NCBI__GCF_900176045.1:WP_084276090.1  
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  280.6   0.1   9.3e-88   9.3e-88      12     338 ..      71     441 ..      62     443 .. 0.91

  Alignments for each domain:
  == domain 1  score: 280.6 bits;  conditional E-value: 9.3e-88
                             TIGR00260  12 ekdlvdlaegstelfrspklaeevga..enlyvkelfhgPtlaFKDrglqfvavlltkalelgne..tvlcAt 80 
                                           +++l++ a   +  f+ p+++ +v +  enl++ el+hgPt aFKD++lq+ + +l+ ++++  e  ++l At
  NCBI__GCF_900176045.1:WP_084276090.1  71 DEELIKEALNLYDKFDDPANPIPVVKveENLFIAELYHGPTRAFKDMALQPFGYILSSLAKARGEnyLILAAT 143
                                           56667777788999999999988877789****************************9987665557****** PP

                             TIGR00260  81 sGdtgaaaaealagkanvkvvvLyPkgkispv.keklvtalaenakvlaikGdFDdaqdlvkeifedke.... 148
                                           sGdtg+a++e ++ ++nvkv ++yP+g++s v   ++vt   en+kv++i+GdFDdaq ++k+++  ++    
  NCBI__GCF_900176045.1:WP_084276090.1 144 SGDTGPATLETFKNRENVKVACIYPAGGTSDVqRLQMVTEDGENLKVIGIEGDFDDAQSALKSLLASEKfhai 216
                                           ********************************99******************************994445889 PP

                             TIGR00260 149 ....klklnsvNsinparieaqk.tyafeiveqlgk...espdkvvvpvpsgnfgailkGflekkelglpiek 213
                                                +kl+++Ns+n++ri +q+ +++++ +e + +      d + + vpsgnfg++l G+++kk++ lpi+k
  NCBI__GCF_900176045.1:WP_084276090.1 217 lqekGIKLSAANSVNFGRIIFQIiYHIHSYLELVRRkeiTFGDTINLIVPSGNFGNALGGYYAKKMG-LPIKK 288
                                           99989***************************99984444579***********************9.***** PP

                             TIGR00260 214 laiaaegaadivrrflksg.dlepkedk.eTlstAmdignpsnverale...larrslgnledlke....... 274
                                           + ia++ + +++++ +++g +++  +++ +T s+Amdi+++snver+l+   +a+r+++ +++l+        
  NCBI__GCF_900176045.1:WP_084276090.1 289 ILIASNAN-NVLTELINEGrYDLRNKHLiKTSSPAMDILKSSNVERVLFdkfGAQRTKELMDSLNDkgyykln 360
                                           ******98.99999988887777777778*******************9999************88******* PP

                             TIGR00260 275 ..............svsdeeileaikklaeeegyllephtavavaalkklvekg..vsatadpaKFeevve.a 330
                                                           +d+e+   ik+ a e+ yl++phta++++  k+ +++    ++ta+  KF+ +v+ a
  NCBI__GCF_900176045.1:WP_084276090.1 361 edelaalqedfaadFSNDDEVKAIIKEYALEKTYLMDPHTATTIKLFKRYAADKnvAYSTAEWTKFAPTVLeA 433
                                           ************9656667777777888889******************999888777***********9999 PP

                             TIGR00260 331 ltgnklkd 338
                                           l g ++k+
  NCBI__GCF_900176045.1:WP_084276090.1 434 LEGGEKKS 441
                                           99988875 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (340 nodes)
Target sequences:                          1  (488 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.00
# Mc/sec: 20.90
//
[ok]

This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis