Alignments for a candidate for aroA in Oleispira antarctica

GapMind for Amino acid biosynthesis

Alignments for a candidate for aroA in Oleispira antarctica

Align 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19) (characterized)
to candidate WP_084687487.1 OLEAN_RS07045 bifunctional prephenate dehydrogenase/3-phosphoshikimate 1-carboxyvinyltransferase

Query= BRENDA::Q83E11
         (438 letters)



>NCBI__GCF_000967895.1:WP_084687487.1
          Length = 748

 Score =  459 bits (1182), Expect = e-133
 Identities = 244/434 (56%), Positives = 306/434 (70%), Gaps = 6/434 (1%)

Query: 1   MDYQTIPSQGLSGEICVPGDKSISHRAVLLAAIAEGQTQVDGFLMGADNLAMVSALQQMG 60
           ++Y   P   LSGE  VPGDKSISHR+++L ++AEG T++ GFL G D+LA + + + MG
Sbjct: 311 VNYVAKPGGKLSGEFRVPGDKSISHRSIMLGSLAEGTTEITGFLEGEDSLATLQSFRDMG 370

Query: 61  ASIQVIEDENILVVEGVGMTGLQAPPEALDCGNSGTAIRLLSGLLAGQPFNTVLTGDSSL 120
             I+      +  V GVG+ GL+ PP AL  GNSGT++RLLSGL++ Q F+  +TGD SL
Sbjct: 371 VVIEGPHRGRV-TVHGVGLHGLKTPPNALYLGNSGTSMRLLSGLMSAQSFDVEMTGDVSL 429

Query: 121 QRRPMKRIIDPLTLMGAKIDSTGNV-PPLKIYGNPRLTGIHYQLPMASAQVKSCLLLAGL 179
            +RPM R+ +PL  MGA I+S     PPLKI G   L GIHY LPMASAQVKSCLLLAGL
Sbjct: 430 SKRPMDRVANPLREMGAVIESADKGRPPLKIKGGQPLKGIHYVLPMASAQVKSCLLLAGL 489

Query: 180 YARGKTCITEPAPSRDHTERLLKHFHYTLQKDKQSICVSGGGKLKANDISIPGDISSAAF 239
           YA G+T + EPAP+RDHTER+LK F Y +  D   + V  GGKL A  I +P DISSAAF
Sbjct: 490 YAEGETTVIEPAPTRDHTERMLKGFGYPVDVDGPKVTVRPGGKLTATAIDVPSDISSAAF 549

Query: 240 FIVAATITPGSAIRLCRVGVNPTRLGVINLLKMMGADIEVTHYTEKNEEPTADITVRHAR 299
           F+VAA+I   S I L  VG+NPTR+GVIN+L+ MGA+IE+ + +E   EP AD+ +R A+
Sbjct: 550 FMVAASIAKDSDITLQHVGINPTRVGVINILRQMGANIEMLNESEVGGEPVADLRIRSAQ 609

Query: 300 LKGIDIPPDQVPLTIDEFPVLLIAAAVAQGKTVLRDAAELRVKETDRIAAMVDGLQKLGI 359
           LKGI IP DQVPL IDEFP + IAAA A+G TVL  A ELRVKE+DRI AMV+G+Q LG+
Sbjct: 610 LKGIQIPEDQVPLAIDEFPAIFIAAACAEGTTVLTGAEELRVKESDRIQAMVEGMQALGV 669

Query: 360 AAESLPDGVIIQG----GTLEGGEVNSYDDHRIAMAFAVAGTLAKGPVRIRNCDNVKTSF 415
            A    DG II G    G+  GGEV+S DDHRIAM+F +A   A  P+ I++C NV TSF
Sbjct: 670 DAVGTEDGAIINGFGSEGSFNGGEVDSVDDHRIAMSFTIASLRANAPILIKDCANVATSF 729

Query: 416 PNFVELANEVGMNV 429
           P FVELA+E G+ V
Sbjct: 730 PGFVELASESGIQV 743


Lambda     K      H
   0.318    0.137    0.392 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 900
Number of extensions: 43
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 438
Length of database: 748
Length adjustment: 36
Effective length of query: 402
Effective length of database: 712
Effective search space:   286224
Effective search space used:   286224
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)

Align candidate WP_084687487.1 OLEAN_RS07045 (bifunctional prephenate dehydrogenase/3-phosphoshikimate 1-carboxyvinyltransferase)
to HMM TIGR01356 (aroA: 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01356.hmm
# target sequence database:        /tmp/gapView.11465.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01356  [M=415]
Accession:   TIGR01356
Description: aroA: 3-phosphoshikimate 1-carboxyvinyltransferase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   6.6e-131  422.9   0.0   8.4e-131  422.5   0.0    1.1  1  lcl|NCBI__GCF_000967895.1:WP_084687487.1  OLEAN_RS07045 bifunctional preph


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000967895.1:WP_084687487.1  OLEAN_RS07045 bifunctional prephenate dehydrogenase/3-phosphoshikimate 1-ca
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  422.5   0.0  8.4e-131  8.4e-131       1     413 [.     323     740 ..     323     742 .. 0.96

  Alignments for each domain:
  == domain 1  score: 422.5 bits;  conditional E-value: 8.4e-131
                                 TIGR01356   1 geikipgsKSishRalllaaLaegetvvtnlLkseDtlatlealrklGakve.eekeelviegvgg..l 66 
                                               ge+++pg+KSishR+++l++Laeg+t++t++L++eD latl+++r++G+ +e  ++++++++gvg   l
  lcl|NCBI__GCF_000967895.1:WP_084687487.1 323 GEFRVPGDKSISHRSIMLGSLAEGTTEITGFLEGEDSLATLQSFRDMGVVIEgPHRGRVTVHGVGLhgL 391
                                               799*************************************************666*********98889 PP

                                 TIGR01356  67 kepeaeldlgnsGttaRlltgvlalasgevvltgdeslkkRPierlveaLrelgaeieskeeegslPla 135
                                               k+p ++l+lgnsGt++Rll+g+++++s++v++tgd sl+kRP++r+ ++Lre+ga ies++ +g++Pl+
  lcl|NCBI__GCF_000967895.1:WP_084687487.1 392 KTPPNALYLGNSGTSMRLLSGLMSAQSFDVEMTGDVSLSKRPMDRVANPLREMGAVIESAD-KGRPPLK 459
                                               **********************************************************999.59***** PP

                                 TIGR01356 136 isgplkg.givelsgsaSsQyksalllaaplalqavtleivgeklisrpyieitLkllksfgveveeed 203
                                               i+g++++ gi+++ ++aS+Q+ks+llla+   l a+++++v+e+  +r+++e++Lk ++     v+++ 
  lcl|NCBI__GCF_000967895.1:WP_084687487.1 460 IKGGQPLkGIHYVLPMASAQVKSCLLLAG---LYAEGETTVIEPAPTRDHTERMLKGFGYP---VDVDG 522
                                               ****9999*********************...77789999999************999877...88876 PP

                                 TIGR01356 204 erkivvkggqkykqkevevegDaSsAafflaaaaitge.evtvenlgenstqgdkaiiivLeemGadve 271
                                                 k++v++g+k + + ++v++D+SsAaff++aa i++  ++t++++g+n+t+ +  +i++L++mGa++e
  lcl|NCBI__GCF_000967895.1:WP_084687487.1 523 P-KVTVRPGGKLTATAIDVPSDISSAAFFMVAASIAKDsDITLQHVGINPTRVG--VINILRQMGANIE 588
                                               5.9********88888**********************9***************..888********** PP

                                 TIGR01356 272 veeqr........dvevegasklkgvkv.didvdsliDelptlavlaafAegetriknieelRvkEsdR 331
                                               + +++        d++++ + +lkg+++ + +v+ +iDe+p++ ++aa+Aeg+t+++++eelRvkEsdR
  lcl|NCBI__GCF_000967895.1:WP_084687487.1 589 MLNESevggepvaDLRIR-SAQLKGIQIpEDQVPLAIDEFPAIFIAAACAEGTTVLTGAEELRVKESDR 656
                                               ***999************.578******99*************************************** PP

                                 TIGR01356 332 iaaiaeeLeklGveveeledgllieGkk..kelkgavvdtydDHRiamalavlglaaegeveiedaecv 398
                                               i+a++e +++lGv++  +edg +i+G     +++g++vd+ dDHRiam++++++l+a++++ i+d + v
  lcl|NCBI__GCF_000967895.1:WP_084687487.1 657 IQAMVEGMQALGVDAVGTEDGAIINGFGseGSFNGGEVDSVDDHRIAMSFTIASLRANAPILIKDCANV 725
                                               ************************9977779************************************** PP

                                 TIGR01356 399 aksfPeFfevleqlg 413
                                               a+sfP F+e++ + g
  lcl|NCBI__GCF_000967895.1:WP_084687487.1 726 ATSFPGFVELASESG 740
                                               *********988765 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (415 nodes)
Target sequences:                          1  (748 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.02
# Mc/sec: 14.47
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis