Align Acetate--CoA ligase CCL3; HlCCL3; 2-methylbutanoate--CoA ligase CCL4; 2-methylpropanoate--CoA ligase CCL4; Butanoate--CoA ligase CCL3; Hexanoate--CoA ligase CCL3; Isovalerate--CoA ligase CCL3; Pentanoate--CoA ligase CCL3; Propionate--CoA ligase CCL3; EC 6.2.1.1; EC 6.2.1.-; EC 6.2.1.17 (characterized)
to candidate WP_086511178.1 BZY95_RS17500 acyl-CoA synthetase
Query= SwissProt::M4IS88 (568 letters) >NCBI__GCF_002151265.1:WP_086511178.1 Length = 543 Score = 593 bits (1530), Expect = e-174 Identities = 295/549 (53%), Positives = 381/549 (69%), Gaps = 16/549 (2%) Query: 10 DLPKNAANYTALTPLWFLERAATVHPTRTSVIHGSRHYTWLQTYHRCRQFASALNNHSIG 69 DLPK AN+ AL+PL F+ER+A+++P +VIHG +W +T+ RCR+ ASAL I Sbjct: 9 DLPKTQANHVALSPLTFIERSASIYPDYPAVIHGDIRRSWRETWARCRRLASALEKRGIK 68 Query: 70 LGSTVAVIAPNVPALYEAHFAVPMAGAVVNCVNIRLNASTIAFLLGHSSAAAVMVDQEFF 129 G TVA + PNVPA++EAHF VP+AG V+N +NIRL+A IA++L H A AV+VD EF Sbjct: 69 PGETVAAMLPNVPAMFEAHFGVPLAGCVLNTLNIRLDAEAIAYMLEHGEAQAVLVDPEFA 128 Query: 130 SLAEEALKILAQESKSHYKPPLLVVIGDESCDPKTLEYALKTGAIEYEKFLEGGDPEFDW 189 + E+A+ LA + PL++ + D + +T G +EYE L GDPE+ + Sbjct: 129 GVIEDAVSRLAIK-------PLVIDVDDALYEGETRHI----GELEYEALLAEGDPEYAY 177 Query: 190 KPPEDEWQSISLGYTSGTTASPKGVVLSHRGAYLMSLSASVVWGINEGAIYLWTLPMFHC 249 + PEDEWQ+ISL YTSGTT PKGVV HRGAYL ++S + W + +YLWTLPMFHC Sbjct: 178 QLPEDEWQAISLNYTSGTTGKPKGVVYHHRGAYLNAVSNILEWAMPHHPVYLWTLPMFHC 237 Query: 250 NGWCYTWGMAAFCGTNICLRQVTAKGVYSAIAKYGVTHFCAAPVVLNTIVNAPPEEAIIP 309 NGWC+ W +AA G ++CLR+V K + I VTHF AP+VLN +VN P E+ Sbjct: 238 NGWCFPWTIAANAGVSVCLRRVDPKKIMDLIVDEKVTHFSGAPIVLNGLVNLPAEQKRA- 296 Query: 310 LPHLVHVMTAGAAPPPSVLFAMSEKGFKVAHTYGLSETYGPSTICAWKPEWDSLPPIKQA 369 H V V TAGAAPP SV+ + + G +V H YGL+E YGP T+CAW+ WD LP ++A Sbjct: 297 FDHPVKVTTAGAAPPASVIAGVEKLGIEVTHVYGLTEVYGPVTVCAWREAWDELPLEERA 356 Query: 370 RLNARQGVRYIALEGLDVVDTKTMKPVPADGTTMGEIVMRGNAVMKGYLKNPKANEESFA 429 R+ ARQGVRY LE L V D T++PVP DG T+GEI+MRGN VMKGYLKN A E++ Sbjct: 357 RIKARQGVRYHMLEALCVADPNTLEPVPKDGETIGEILMRGNNVMKGYLKNEAATEQALE 416 Query: 430 DGWFHSGDLAVKHPDGYIEIKDRSKDIIISGGENISSLEVENTLYLHPAVLEVSVVARPD 489 GW+H+GDLAV HPDGYIEIKDRSKDIIISGGENIS++EVE+ +Y HPAV E +VVA+PD Sbjct: 417 GGWYHTGDLAVWHPDGYIEIKDRSKDIIISGGENISTIEVEDAIYSHPAVEEAAVVAKPD 476 Query: 490 ERWGESPCAFVTLKPNIDKSNEQVLAEDIIKFCKSKMPAYWVPKSVVFGPLPKTATGKIQ 549 E+WGE+PCAFV LK +V DII+ C+ + + VPK+V+F LPKT+TGKIQ Sbjct: 477 EKWGETPCAFVKLKVGYG----EVTEADIIEHCRQHLARFKVPKTVIFTELPKTSTGKIQ 532 Query: 550 KHVLRAKAK 558 K VLR +A+ Sbjct: 533 KFVLREEAR 541 Lambda K H 0.318 0.134 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 925 Number of extensions: 36 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 568 Length of database: 543 Length adjustment: 36 Effective length of query: 532 Effective length of database: 507 Effective search space: 269724 Effective search space used: 269724 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory