Align 3-isopropylmalate dehydratase small subunit 2; EC 4.2.1.33; Alpha-IPM isomerase 2; IPMI 2; Isopropylmalate isomerase 2 (uncharacterized)
to candidate WP_089301396.1 CHB84_RS10720 aconitate hydratase
Query= curated2:Q9V1I9 (163 letters) >NCBI__GCF_900188115.1:WP_089301396.1 Length = 936 Score = 51.2 bits (121), Expect = 5e-11 Identities = 41/127 (32%), Positives = 63/127 (49%), Gaps = 25/127 (19%) Query: 54 VVVGGKNFGIGSSRESAALALKAAGVSGIIAKSFGRIFYRNAVNLGI------------P 101 VV+GGK +G GSSR+ AA + GV +IA+SF RI N + +G+ Sbjct: 806 VVLGGKEYGSGSSRDWAAKGTRLLGVRAVIAESFERIHRSNLIGMGVIPLQFPSGESADS 865 Query: 102 LLIGDTDELEDGDVITVN----WETGEV---RKNGQTLQFE-----PLPGFLLEIVREGG 149 L I T+ + + +N ET V R++G T++F+ PG + R GG Sbjct: 866 LGIDGTETFDISGITALNDGSTPETVRVTATRQDGSTVRFDADVRIDTPG-EADYYRNGG 924 Query: 150 ILEFIRR 156 IL+++ R Sbjct: 925 ILQYVLR 931 Lambda K H 0.321 0.142 0.425 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 348 Number of extensions: 14 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 163 Length of database: 936 Length adjustment: 30 Effective length of query: 133 Effective length of database: 906 Effective search space: 120498 Effective search space used: 120498 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 50 (23.9 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory