Align glutamyl-tRNAGln amidotransferase subunit B (EC 6.3.5.7) (characterized)
to candidate WP_089322288.1 CHB58_RS01305 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB
Query= metacyc::MONOMER-13956 (476 letters) >NCBI__GCF_900188395.1:WP_089322288.1 Length = 485 Score = 479 bits (1233), Expect = e-140 Identities = 244/486 (50%), Positives = 342/486 (70%), Gaps = 11/486 (2%) Query: 1 MNFETVIGLEVHVELKTKSKIFSSSPTPFGAEANTQTSVIDLGYPGVLPVLNKEAVEFAM 60 M FE VIGLEVH +L T +KIF S FG NT + LG PG LPVLNK+AVE+A+ Sbjct: 1 MEFEAVIGLEVHAQLLTDTKIFCSCRNRFGDPPNTNVCPVCLGMPGSLPVLNKKAVEYAV 60 Query: 61 KAAMALNCEIATDTKFDRKNYFYPDNPKAYQISQFDKPIGENGWIEIEV-GGKTKRIGIT 119 KAA+ALNC+I T + F RK+YFYPD PKAYQI+Q++ P E+GWIEIE G K+I I Sbjct: 61 KAALALNCKINTYSIFARKHYFYPDLPKAYQITQYELPFAEHGWIEIEKPDGTKKKIRIR 120 Query: 120 RLHLEEDAGKLTHTGDGY---SLVDFNRQGTPLVEIVSEPDIRTPEEAYAYLEKLKSIIQ 176 R+H+EEDAGK H G+GY S VD NR GTPL+EIVSEPDI +PEEA Y++KL+ I+ Sbjct: 121 RIHMEEDAGKTLH-GEGYDQNSYVDLNRAGTPLIEIVSEPDISSPEEARLYMQKLRDILV 179 Query: 177 YTGVSDCKMEEGSLRCDANISLRPIGQEEFGTKTELKNLNSFAFVQKGLEHEEKRQEQVL 236 + GV+D +EEGSLRCDAN+S+RP G ++ GT+TE+KN+NSF F+QK LE+E +RQ +V+ Sbjct: 180 WIGVNDGNLEEGSLRCDANVSIRPKGSDKLGTRTEIKNVNSFRFIQKALEYEIERQIKVV 239 Query: 237 LSGFFIQQETRRYDEATKKTILMRVKEGSDDYRYFPEPDLVELYIDDEWKERVKASIPEL 296 G + QETR YD T MR KE ++DYRYFPEPDL L ID EW + +KAS+PEL Sbjct: 240 KGGGEVVQETRLYDSQKNITKTMRTKEEAEDYRYFPEPDLPPLIIDSEWLDSIKASLPEL 299 Query: 297 PDERRKRYIEELGFAAYDAMVLTLTKEMADFFEETVQK-GAEAKQASNWLMGEVSAYLNA 355 PD ++R++++ YDA +L + +A FFE V E K+ +N+++ + LN Sbjct: 300 PDSVKERFVKDYSLTPYDASILVKERALAHFFESAVSSYSGEPKKVANFIISDFLGILNE 359 Query: 356 EQKELADVALTPEGLAGMIKLIEKGTISSKIAKK-VFKELIEKGGDAEKIVKEKGLVQIS 414 E+ ++DV + PE +A ++ L++KGTIS ++AK+ V E+++ G EKIVKEKGLVQIS Sbjct: 360 EKIGISDVKVKPEYIAELLTLVDKGTISLRVAKEDVLPEMVKTGKSPEKIVKEKGLVQIS 419 Query: 415 DEGVLLKLVTEALDNNPQSIEDFKNGKD----RAIGFLVGQIMKASKGQANPPMVNKILL 470 DE L++++ L+ N +++ +K G D +A+ FL+GQ+MK +KG+ANP ++N+++ Sbjct: 420 DESALVEIIEAVLNKNEKAVNQYKTGDDKKKTKAVKFLIGQVMKETKGKANPKVLNELIP 479 Query: 471 EEIKKR 476 + + K+ Sbjct: 480 KILDKK 485 Lambda K H 0.314 0.134 0.373 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 687 Number of extensions: 36 Number of successful extensions: 6 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 476 Length of database: 485 Length adjustment: 34 Effective length of query: 442 Effective length of database: 451 Effective search space: 199342 Effective search space used: 199342 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 51 (24.3 bits)
Align candidate WP_089322288.1 CHB58_RS01305 (Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB)
to HMM TIGR00133 (gatB: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, B subunit (EC 6.3.5.-))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00133.hmm # target sequence database: /tmp/gapView.26156.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00133 [M=481] Accession: TIGR00133 Description: gatB: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, B subunit Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.1e-193 630.0 0.5 1.3e-193 629.9 0.5 1.0 1 lcl|NCBI__GCF_900188395.1:WP_089322288.1 CHB58_RS01305 Asp-tRNA(Asn)/Glu- Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_900188395.1:WP_089322288.1 CHB58_RS01305 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 629.9 0.5 1.3e-193 1.3e-193 2 480 .. 1 482 [. 1 483 [. 0.97 Alignments for each domain: == domain 1 score: 629.9 bits; conditional E-value: 1.3e-193 TIGR00133 2 ieyelviGlEvHvqlntksKlFckcsneaqeakpNtnvcpvclglPGalPvlNkeavkkAlklalalns 70 +e+e+viGlEvH ql t +K+Fc+c n++ + +pNtnvcpvclg+PG+lPvlNk+av+ A+k alaln+ lcl|NCBI__GCF_900188395.1:WP_089322288.1 1 MEFEAVIGLEVHAQLLTDTKIFCSCRNRFGD-PPNTNVCPVCLGMPGSLPVLNKKAVEYAVKAALALNC 68 689****************************.************************************* PP TIGR00133 71 kivsevsvFdRKhYfYpDlPkgyqitqqdlPiaedGkleieleeke.keigierlhlEeDtgksqykes 138 ki+ +s+F+RKhYfYpDlPk+yqitq++lP+ae+G +eie+ ++ k+i+i+r+h+EeD+gk+ + e lcl|NCBI__GCF_900188395.1:WP_089322288.1 69 KIN-TYSIFARKHYFYPDLPKAYQITQYELPFAEHGWIEIEKPDGTkKKIRIRRIHMEEDAGKTLHGEG 136 *77.5************************************98765499**************999865 PP TIGR00133 139 dkdkqslvDfNRsgvPLlEiVtkPdlksakearaflkklrqilryleisdgdleeGsmRvDvNvsirlk 207 d+ s+vD+NR+g+PL+EiV++Pd++s++ear +++klr il ++++ dg+leeGs+R+D+Nvsir+k lcl|NCBI__GCF_900188395.1:WP_089322288.1 137 -YDQNSYVDLNRAGTPLIEIVSEPDISSPEEARLYMQKLRDILVWIGVNDGNLEEGSLRCDANVSIRPK 204 .589***************************************************************** PP TIGR00133 208 GqekegtrvEiKNlnslksiekaieyEieRqkkllkkgeevkqetrafdekksitvslRkKeeseDYRY 276 G++k gtr EiKN+ns++ i+ka+eyEieRq+k++k g ev+qetr +d +k+it ++R Kee+eDYRY lcl|NCBI__GCF_900188395.1:WP_089322288.1 205 GSDKLGTRTEIKNVNSFRFIQKALEYEIERQIKVVKGGGEVVQETRLYDSQKNITKTMRTKEEAEDYRY 273 ********************************************************************* PP TIGR00133 277 fpePdlppieideevvkekveeklpelPeakrirlkkeyglseedakvlvsdlelldafeevvkl.ike 344 fpePdlpp+ id e++++ +++ lpelP+ ++r++k+y l +da +lv+++ l++ fe++v++ ++e lcl|NCBI__GCF_900188395.1:WP_089322288.1 274 FPEPDLPPLIIDSEWLDS-IKASLPELPDSVKERFVKDYSLTPYDASILVKERALAHFFESAVSSySGE 341 ***************999.9****************************************987661678 PP TIGR00133 345 pklavnWileellgeLnkkkislaeallkpeelaeliklikegkisqksake.lleellenkkdpkkli 412 pk+++n+i++++lg Ln++ki ++++ +kpe++ael+ l+++g+is + ake +l e+++++k+p+k++ lcl|NCBI__GCF_900188395.1:WP_089322288.1 342 PKKVANFIISDFLGILNEEKIGISDVKVKPEYIAELLTLVDKGTISLRVAKEdVLPEMVKTGKSPEKIV 410 8*************************************************955**************** PP TIGR00133 413 eklgliqisdekelvkiveevikenpkevekyk....sgkekalkflvGqvmkktkgradpkevekllk 477 +++gl+qisde++lv+i+e+v+++n+k+v +yk ++k ka+kfl+Gqvmk+tkg+a+pk ++l+ lcl|NCBI__GCF_900188395.1:WP_089322288.1 411 KEKGLVQISDESALVEIIEAVLNKNEKAVNQYKtgddKKKTKAVKFLIGQVMKETKGKANPKVLNELIP 479 *********************************998888999**************************9 PP TIGR00133 478 ell 480 ++l lcl|NCBI__GCF_900188395.1:WP_089322288.1 480 KIL 482 998 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (481 nodes) Target sequences: 1 (485 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.02 # Mc/sec: 9.83 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory