Align glutamate 5-kinase (EC 2.7.2.11) (characterized)
to candidate WP_090439933.1 BLS63_RS03675 glutamate 5-kinase
Query= BRENDA::P0A7B5 (367 letters) >NCBI__GCF_900100495.1:WP_090439933.1 Length = 372 Score = 297 bits (760), Expect = 4e-85 Identities = 161/359 (44%), Positives = 227/359 (63%), Gaps = 1/359 (0%) Query: 8 VVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHRIVIVTSGAIAAGREHLGYPELPA 67 VVK+G+++LT R L+RA + VRQ L G +V+V+SGA+AAG LG+ P+ Sbjct: 12 VVKIGSALLTADGRGLDRAAMAVWVRQMVALREQGVELVLVSSGAVAAGMSRLGWTARPS 71 Query: 68 TIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQMLLTRADMEDRERFLNARDTLRALLDN 127 + Q AA+GQ LIQ WE F+ +G Q+LLT D+ DR+R+LNAR TLR L++ Sbjct: 72 AMHELQAAAAIGQMVLIQAWESSFAEHGRRTAQILLTHDDLSDRKRYLNARSTLRTLVEL 131 Query: 128 NIVPVINENDAVATAEIKVGDNDNLSALAAILAGADKLLLLTDQKGLYTADPRSNPQAEL 187 ++VPVINEND V T EI+ GDND L+AL A L AD L++LTD+ G+Y ADPR NP AEL Sbjct: 132 DVVPVINENDTVVTDEIRFGDNDTLAALVANLVEADLLVILTDRDGMYNADPRHNPGAEL 191 Query: 188 IKDVYGIDDALRAIAGDSVSGLGTGGMSTKLQAADVACRAGIDTIIAAGSKPGVIGDVME 247 I + D AL A+AG LG GGM TKL+AA +A R+G T+I G+ V+ + Sbjct: 192 IYEARADDPALDAVAGGVGGALGRGGMQTKLRAARLAARSGAHTVIVGGAIEQVLARLRS 251 Query: 248 GISVGTLFHAQATPLENRKRWIFG-APPAGEITVDEGATAAILERGSSLLPKGIKSVTGN 306 G +GTL + + L RK+W+ G G + +D GA A++ SLLP G+K+V G+ Sbjct: 252 GERLGTLLAPERSLLAARKQWLAGHLQTRGTLVLDAGAVKALVSDRKSLLPVGVKAVQGS 311 Query: 307 FSRGEVIRICNLEGRDIAHGVSRYNSDALRRIAGHHSQEIDAILGYEYGPVAVHRDDMI 365 F RGE++ +GR+IA G++ Y++ ++I G S+ I+ +LGY P VHRD++I Sbjct: 312 FRRGEMVVCVGPDGREIARGLANYSALEAQKIIGQSSEAIERLLGYVDEPELVHRDNLI 370 Lambda K H 0.318 0.135 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 359 Number of extensions: 14 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 367 Length of database: 372 Length adjustment: 30 Effective length of query: 337 Effective length of database: 342 Effective search space: 115254 Effective search space used: 115254 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 49 (23.5 bits)
Align candidate WP_090439933.1 BLS63_RS03675 (glutamate 5-kinase)
to HMM TIGR01027 (proB: glutamate 5-kinase (EC 2.7.2.11))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR01027.hmm # target sequence database: /tmp/gapView.4632.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01027 [M=363] Accession: TIGR01027 Description: proB: glutamate 5-kinase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 2.3e-135 437.4 0.2 2.6e-135 437.2 0.2 1.0 1 lcl|NCBI__GCF_900100495.1:WP_090439933.1 BLS63_RS03675 glutamate 5-kinase Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_900100495.1:WP_090439933.1 BLS63_RS03675 glutamate 5-kinase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 437.2 0.2 2.6e-135 2.6e-135 2 362 .. 10 370 .. 9 371 .. 0.99 Alignments for each domain: == domain 1 score: 437.2 bits; conditional E-value: 2.6e-135 TIGR01027 2 riVvKlGsssLteesgklkrsklaelveqvaklkkaGhevvivsSGavaaGleaLglperpkklaekQa 70 r VvK+Gs++Lt++ + l+r+ +a v+q+ +l+++G e+v+vsSGavaaG+++Lg + rp ++e Qa lcl|NCBI__GCF_900100495.1:WP_090439933.1 10 RWVVKIGSALLTADGRGLDRAAMAVWVRQMVALREQGVELVLVSSGAVAAGMSRLGWTARPSAMHELQA 78 67******************************************************************* PP TIGR01027 71 laaVGQgrLmklyeklfsqyglkvaQiLLtradlskrerylNarntleellelgvvpivNENDtvavee 139 +aa+GQ L++++e++f+++g+++aQiLLt++dls+r+rylNar+tl++l+el+vvp++NENDtv ++e lcl|NCBI__GCF_900100495.1:WP_090439933.1 79 AAAIGQMVLIQAWESSFAEHGRRTAQILLTHDDLSDRKRYLNARSTLRTLVELDVVPVINENDTVVTDE 147 ********************************************************************* PP TIGR01027 140 ikfGDNDtLsalvaalveAdlLvlltdvdgLydadprtnpdAklieeveeieeelkavagssgssvGTG 208 i+fGDNDtL+alva+lveAdlLv+ltd dg+y+adpr+np A+li e ++ + +l+avag+ g ++G G lcl|NCBI__GCF_900100495.1:WP_090439933.1 148 IRFGDNDTLAALVANLVEADLLVILTDRDGMYNADPRHNPGAELIYEARADDPALDAVAGGVGGALGRG 216 ********************************************************************* PP TIGR01027 209 GmrtKleaaelAsragveviiasgekpekiadlledaavgtlfeakkkklknrkqwilaaseakGkiiv 277 Gm+tKl aa+lA+r+g +++i+ g+ ++ +a+l +++ +gtl++++++ l +rkqw++ +++++G++++ lcl|NCBI__GCF_900100495.1:WP_090439933.1 217 GMQTKLRAARLAARSGAHTVIVGGAIEQVLARLRSGERLGTLLAPERSLLAARKQWLAGHLQTRGTLVL 285 ********************************************************************* PP TIGR01027 278 degaeeallekgksLlpagvvevegnFsrgevveilaeegqeigkglvnysseelekikglkseeiedv 346 d+ga++al++ +ksLlp+gv +v+g+F+rge+v ++ ++g+ei++gl+nys+ e +ki g++se ie + lcl|NCBI__GCF_900100495.1:WP_090439933.1 286 DAGAVKALVSDRKSLLPVGVKAVQGSFRRGEMVVCVGPDGREIARGLANYSALEAQKIIGQSSEAIERL 354 ********************************************************************* PP TIGR01027 347 Lgyekkeevvhrdnlv 362 Lgy ++ e vhrdnl+ lcl|NCBI__GCF_900100495.1:WP_090439933.1 355 LGYVDEPELVHRDNLI 370 **************97 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (363 nodes) Target sequences: 1 (372 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.02 # Mc/sec: 6.74 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory