Alignments for a candidate for hisC in Pseudomonas benzenivorans DSM 8628

GapMind for Amino acid biosynthesis

Alignments for a candidate for hisC in Pseudomonas benzenivorans DSM 8628

Align histidinol-phosphate aminotransferase (EC 2.6.1.9) (characterized)
to candidate WP_090447996.1 BLS63_RS22285 histidinol-phosphate transaminase

Query= reanno::psRCH2:GFF3234
         (348 letters)



>NCBI__GCF_900100495.1:WP_090447996.1
          Length = 350

 Score =  613 bits (1582), Expect = e-180
 Identities = 304/347 (87%), Positives = 323/347 (93%)

Query: 1   MSKFWSPFVKDLVPYVPGEQPKLSKLVKLNTNENPYGPSPRAIAAMQAELNDSLRLYPDP 60
           MSKFWSPFVKDLVPYVPGEQPKL+KLVKLNTNENPYGPSP+AIAAMQAEL D+LRLYPDP
Sbjct: 1   MSKFWSPFVKDLVPYVPGEQPKLAKLVKLNTNENPYGPSPKAIAAMQAELGDNLRLYPDP 60

Query: 61  NGERLKQAVADYYGVQPAQVFVGNGSDEVLAHAFHGLFQHAGPLLFPDISYSFYPVYCGL 120
           NGERLKQAVADYYGV PAQVFVGNGSDEVLAHAFHGLFQH  PLLFPDISYSFYPVYCGL
Sbjct: 61  NGERLKQAVADYYGVTPAQVFVGNGSDEVLAHAFHGLFQHGRPLLFPDISYSFYPVYCGL 120

Query: 121 YGIAYETVALDEQFQIDVADYNRPNGGIIFPNPNAPTGCLLALEAIERLLQANTETVVLV 180
           YGI +E VALDEQFQI V DY RPNGGI+FPNPNAPTGCLL L AIERLL+AN ++VVLV
Sbjct: 121 YGIPFEAVALDEQFQIRVEDYARPNGGIVFPNPNAPTGCLLPLTAIERLLEANPDSVVLV 180

Query: 181 DEAYVDFGGESAIALVDRYPNLLVTQTLSKSRSLAGLRVGLAVGHPDLIEALERIKNSFN 240
           DEAYVDFGGE+AI+LVD+YPNLLVTQTLSKSRSLAGLRVGLAVGHP+LIEALERIKNSFN
Sbjct: 181 DEAYVDFGGETAISLVDKYPNLLVTQTLSKSRSLAGLRVGLAVGHPELIEALERIKNSFN 240

Query: 241 SYPLDRIAIAGAAAAFEDRAYFQQTCQQVIDSREAVVAAMQGLGFEVLPSAANFIFARHP 300
           SYPLDR+AIAGAAAAF DR YF +TCQ VI SREAVVA +Q LGFEVLPSAANF+FARHP
Sbjct: 241 SYPLDRLAIAGAAAAFADREYFAKTCQAVIASREAVVAGLQQLGFEVLPSAANFVFARHP 300

Query: 301 QRDAATIAASLREQGVIVRHFKQRRIEQFLRITIGTPEQNQALLEAL 347
           QRDAA +AA LREQGVIVRHFKQ RI QFLRI+IG PEQNQALL+AL
Sbjct: 301 QRDAAELAAQLREQGVIVRHFKQARIAQFLRISIGAPEQNQALLDAL 347


Lambda     K      H
   0.320    0.137    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 566
Number of extensions: 14
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 348
Length of database: 350
Length adjustment: 29
Effective length of query: 319
Effective length of database: 321
Effective search space:   102399
Effective search space used:   102399
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 49 (23.5 bits)

Align candidate WP_090447996.1 BLS63_RS22285 (histidinol-phosphate transaminase)
to HMM TIGR01141 (hisC: histidinol-phosphate transaminase (EC 2.6.1.9))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01141.hmm
# target sequence database:        /tmp/gapView.10044.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01141  [M=349]
Accession:   TIGR01141
Description: hisC: histidinol-phosphate transaminase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
    7.1e-97  310.3   0.0    7.9e-97  310.2   0.0    1.0  1  lcl|NCBI__GCF_900100495.1:WP_090447996.1  BLS63_RS22285 histidinol-phospha


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_900100495.1:WP_090447996.1  BLS63_RS22285 histidinol-phosphate transaminase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  310.2   0.0   7.9e-97   7.9e-97       3     347 ..       8     348 ..       6     350 .] 0.95

  Alignments for each domain:
  == domain 1  score: 310.2 bits;  conditional E-value: 7.9e-97
                                 TIGR01141   3 kikklepYqpgarelgekevvkLnsnEnPfgpsekvkealkeelk.klhrYpdpqalelkealakylgv 70 
                                                +k+l pY+pg++ +   + vkLn+nEnP+gps+k+++a+++el  +l++Ypdp++ +lk+a+a+y+gv
  lcl|NCBI__GCF_900100495.1:WP_090447996.1   8 FVKDLVPYVPGEQPKLA-KLVKLNTNENPYGPSPKAIAAMQAELGdNLRLYPDPNGERLKQAVADYYGV 75 
                                               699*******9777766.5*************************88*********************** PP

                                 TIGR01141  71 eeenillgnGsdelielliraflepgdavlvleptysmYevsakiagaevkevplkedgqedleavlea 139
                                               +++++++gnGsde++   ++ + + g+  l+++ +ys Y+v+  ++g+    v+l+e++q  +e+ +  
  lcl|NCBI__GCF_900100495.1:WP_090447996.1  76 TPAQVFVGNGSDEVLAHAFHGLFQHGRPLLFPDISYSFYPVYCGLYGIPFEAVALDEQFQIRVEDYA-- 142
                                               **********************************************************988888877.. PP

                                 TIGR01141 140 akekvklvflasPnnPtGnllkreeiekvleevedalVVvDeAYieFseeasvlellaeypnlvvlrTl 208
                                                  +  +vf  +Pn+PtG ll    ie++le+  d +V+vDeAY++F +e ++++l+ +ypnl+v++Tl
  lcl|NCBI__GCF_900100495.1:WP_090447996.1 143 -RPNGGIVF-PNPNAPTGCLLPLTAIERLLEANPDSVVLVDEAYVDFGGE-TAISLVDKYPNLLVTQTL 208
                                               .66666776.89********************77***************7.****************** PP

                                 TIGR01141 209 SKafgLAglRvGyaianaeiiealekvrapyn...vsslaleaavaalrdsdkiektveevkkererll 274
                                               SK+ +LAglRvG+a++++e+ieale++++++n   +++la + a aa  d+++++kt ++v+++re + 
  lcl|NCBI__GCF_900100495.1:WP_090447996.1 209 SKSRSLAGLRVGLAVGHPELIEALERIKNSFNsypLDRLAIAGAAAAFADREYFAKTCQAVIASREAVV 277
                                               ****************************87543339********************************* PP

                                 TIGR01141 275 eelkkleglevyeSkaNFvlikvke.daeelleallekgiivRdlksaeglleeclRitvGtreenerl 342
                                               ++l++l g+ev +S aNFv+++ ++ da+el+ +l e+g+ivR++k+a   + ++lRi++G +e+n++l
  lcl|NCBI__GCF_900100495.1:WP_090447996.1 278 AGLQQL-GFEVLPSAANFVFARHPQrDAAELAAQLREQGVIVRHFKQA--RIAQFLRISIGAPEQNQAL 343
                                               ******.8***************999********************88..489**************** PP

                                 TIGR01141 343 lealk 347
                                               l+al+
  lcl|NCBI__GCF_900100495.1:WP_090447996.1 344 LDALQ 348
                                               **997 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (349 nodes)
Target sequences:                          1  (350 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.01
# Mc/sec: 9.06
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis