Align acetolactate synthase (subunit 2/2) (EC 2.2.1.6) (characterized)
to candidate WP_090448334.1 BLS63_RS24040 acetolactate synthase 3 large subunit
Query= BRENDA::P00893 (574 letters) >NCBI__GCF_900100495.1:WP_090448334.1 Length = 574 Score = 765 bits (1975), Expect = 0.0 Identities = 370/576 (64%), Positives = 455/576 (78%), Gaps = 4/576 (0%) Query: 1 MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGIDHVLVRHEQAAVHMADG 60 ME+LSGAEMVVR L D+GVK ++GYPGGA+L IYDAL ++H+LVRHEQAA HMADG Sbjct: 1 MELLSGAEMVVRFLRDEGVKNIYGYPGGALLHIYDALFKEPEVNHILVRHEQAATHMADG 60 Query: 61 LARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQVATSLIGYDAFQECDMVG 120 ARATG+ GVVLVTSGPGATNAITGIATAYMDSIP+VVLSGQV ++++G DAFQE DM+G Sbjct: 61 YARATGKAGVVLVTSGPGATNAITGIATAYMDSIPMVVLSGQVPSNMVGTDAFQETDMIG 120 Query: 121 ISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVDLPKDILNPANKLPYVWPESV 180 ISRP+VKHSF++K +IP+V+KKAF+LA SGRPGPVVVD+PKD+ NPA K YV+P+ Sbjct: 121 ISRPIVKHSFMIKHASEIPEVMKKAFYLAQSGRPGPVVVDIPKDMTNPAEKFEYVYPKKA 180 Query: 181 SMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAITAGCHQQLKETVEALNLPVVCS 240 +RSY+P GH GQI++A + L++AK+P++Y GGG I G L E + LNLPV + Sbjct: 181 KLRSYSPALRGHSGQIRKAAELLLSAKRPILYSGGGVIMGGAAAPLTELAQMLNLPVTNT 240 Query: 241 LMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVIFAVGVRFDDRTTNNLAKYCPNATV 300 LMGLG +P T RQ LGMLGMHG+Y AN+ MH+ADVI AVG RFDDR N K+CPNA + Sbjct: 241 LMGLGGYPGTDRQFLGMLGMHGSYTANLAMHHADVILAVGARFDDRVINGANKFCPNAKI 300 Query: 301 LHIDIDPTSISKTVTADIPIVGDARQVLEQMLELLSQESAHQPLDEIRDWWQQIEQWR-A 359 +HIDIDP SISKT+ ADIPIVG VL +M+ ++ + + + + WW+QI++WR + Sbjct: 301 IHIDIDPASISKTIKADIPIVGPVDSVLTEMVAIVKEIGENLSKEALSSWWKQIDEWRGS 360 Query: 360 RQCLKYDT-HSEKIKPQAVIETLWRLTKGDAYVTSDVGQHQMFAALYYPFDKPRRWINSG 418 R YD IKPQ VIETL +T+GDA+VTSDVGQHQMFAA YY F+KP RWINSG Sbjct: 361 RGMFPYDKGDGSIIKPQTVIETLCEVTRGDAFVTSDVGQHQMFAAQYYRFNKPNRWINSG 420 Query: 419 GLGTMGFGLPAALGVKMALPEETVVCVTGDGSIQMNIQELSTALQYELPVLVVNLNNRYL 478 GLGTMGFGLPAA+GVK+ P++ V CVTG+GSIQMNIQELST LQY+LPV +VNLNN L Sbjct: 421 GLGTMGFGLPAAMGVKLNFPDDDVACVTGEGSIQMNIQELSTCLQYDLPVKIVNLNNGAL 480 Query: 479 GMVKQWQDMIYSGRHSQSYMQSLPDFVRLAEAYGHVGIQISHPHELESKLSEALEQVRNN 538 GMV+QWQDM+YS RHS SYM+SLPDF++LAEAYGHVG++I+ +L+ K+ EA + Sbjct: 481 GMVRQWQDMMYSSRHSHSYMESLPDFIKLAEAYGHVGMRITELKDLKPKMEEAF--ALKD 538 Query: 539 RLVFVDVTVDGSEHVYPMQIRGGGMDEMWLSKTERT 574 RLVF+D+ VD SEHVYPMQI+ G M +MWLSKTERT Sbjct: 539 RLVFLDIQVDTSEHVYPMQIKDGAMRDMWLSKTERT 574 Lambda K H 0.319 0.135 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1032 Number of extensions: 37 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 574 Length of database: 574 Length adjustment: 36 Effective length of query: 538 Effective length of database: 538 Effective search space: 289444 Effective search space used: 289444 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 53 (25.0 bits)
Align candidate WP_090448334.1 BLS63_RS24040 (acetolactate synthase 3 large subunit)
to HMM TIGR00118 (ilvB: acetolactate synthase, large subunit, biosynthetic type (EC 2.2.1.6))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00118.hmm # target sequence database: /tmp/gapView.14631.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00118 [M=557] Accession: TIGR00118 Description: acolac_lg: acetolactate synthase, large subunit, biosynthetic type Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.2e-261 855.0 0.3 1.4e-261 854.8 0.3 1.0 1 lcl|NCBI__GCF_900100495.1:WP_090448334.1 BLS63_RS24040 acetolactate synth Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_900100495.1:WP_090448334.1 BLS63_RS24040 acetolactate synthase 3 large subunit # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 854.8 0.3 1.4e-261 1.4e-261 1 556 [. 4 569 .. 4 570 .. 0.97 Alignments for each domain: == domain 1 score: 854.8 bits; conditional E-value: 1.4e-261 TIGR00118 1 lkgaeilveslkkegvetvfGyPGGavlpiydaly.dselehilvrheqaaahaadGyarasGkvGvvl 68 l+gae++v+ l++egv+ ++GyPGGa+l+iydal+ + e++hilvrheqaa+h+adGyara+Gk+Gvvl lcl|NCBI__GCF_900100495.1:WP_090448334.1 4 LSGAEMVVRFLRDEGVKNIYGYPGGALLHIYDALFkEPEVNHILVRHEQAATHMADGYARATGKAGVVL 72 68*********************************889******************************* PP TIGR00118 69 atsGPGatnlvtgiatayldsvPlvvltGqvatsliGsdafqeidilGitlpvtkhsflvkkaedlpei 137 +tsGPGatn++tgiatay+ds+P+vvl+Gqv+++++G+dafqe d++Gi++p++khsf++k+a+++pe+ lcl|NCBI__GCF_900100495.1:WP_090448334.1 73 VTSGPGATNAITGIATAYMDSIPMVVLSGQVPSNMVGTDAFQETDMIGISRPIVKHSFMIKHASEIPEV 141 ********************************************************************* PP TIGR00118 138 lkeafeiastGrPGPvlvdlPkdvteaeieleve..ekvelpgykptvkghklqikkaleliekakkPv 204 +k+af++a++GrPGPv+vd+Pkd+t+++ ++e+ +k +l++y p ++gh qi+ka+el+ +ak+P+ lcl|NCBI__GCF_900100495.1:WP_090448334.1 142 MKKAFYLAQSGRPGPVVVDIPKDMTNPAEKFEYVypKKAKLRSYSPALRGHSGQIRKAAELLLSAKRPI 210 *************************99888777666********************************* PP TIGR00118 205 llvGgGviiaeaseelkelaerlkipvtttllGlGafpedhplalgmlGmhGtkeanlavseadlliav 273 l+ GgGvi+++a + l+ela++l++pvt tl+GlG++p +++++lgmlGmhG+++anla+++ad+++av lcl|NCBI__GCF_900100495.1:WP_090448334.1 211 LYSGGGVIMGGAAAPLTELAQMLNLPVTNTLMGLGGYPGTDRQFLGMLGMHGSYTANLAMHHADVILAV 279 ********************************************************************* PP TIGR00118 274 GarfddrvtgnlakfapeakiihididPaeigknvkvdipivGdakkvleellkklkee....ekkeke 338 Garfddrv + +kf+p+akiihididPa+i+k++k+dipivG + +vl+e+++ +ke +k+ lcl|NCBI__GCF_900100495.1:WP_090448334.1 280 GARFDDRVINGANKFCPNAKIIHIDIDPASISKTIKADIPIVGPVDSVLTEMVAIVKEIgenlSKEALS 348 *****************************************************9999887766455545 PP TIGR00118 339 .Wlekieewkkeyilk.ldeeees.ikPqkvikelskllkdeaivttdvGqhqmwaaqfyktkkprkfi 404 W+++i+ew+ + + +d+ ++s ikPq+vi++l+++++++a+vt+dvGqhqm+aaq+y+++kp+++i lcl|NCBI__GCF_900100495.1:WP_090448334.1 349 sWWKQIDEWRGSRGMFpYDKGDGSiIKPQTVIETLCEVTRGDAFVTSDVGQHQMFAAQYYRFNKPNRWI 417 6*********8775554999888879******************************************* PP TIGR00118 405 tsgGlGtmGfGlPaalGakvakpeetvvavtGdgsfqmnlqelstiveydipvkivilnnellGmvkqW 473 +sgGlGtmGfGlPaa+G+k+ p+++v +vtG+gs+qmn+qelst+ +yd+pvkiv+lnn lGmv+qW lcl|NCBI__GCF_900100495.1:WP_090448334.1 418 NSGGLGTMGFGLPAAMGVKLNFPDDDVACVTGEGSIQMNIQELSTCLQYDLPVKIVNLNNGALGMVRQW 486 ********************************************************************* PP TIGR00118 474 qelfyeerysetklaselpdfvklaeayGvkgiriekpeeleeklkealesk.epvlldvevdkeeevl 541 q+++y+ r+s+++++s lpdf+klaeayG++g+ri++ ++l+ k++ea++ k ++v+ld++vd++e+v+ lcl|NCBI__GCF_900100495.1:WP_090448334.1 487 QDMMYSSRHSHSYMES-LPDFIKLAEAYGHVGMRITELKDLKPKMEEAFALKdRLVFLDIQVDTSEHVY 554 ***************5.*******************************9987699************** PP TIGR00118 542 PmvapGagldelvee 556 Pm+++ +++ +++ + lcl|NCBI__GCF_900100495.1:WP_090448334.1 555 PMQIKDGAMRDMWLS 569 ************976 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (557 nodes) Target sequences: 1 (574 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.03u 0.01s 00:00:00.04 Elapsed: 00:00:00.04 # Mc/sec: 7.38 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory