Align phosphoribosylanthranilate isomerase (EC 5.3.1.24) (characterized)
to candidate WP_093393958.1 BM091_RS05045 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase
Query= BRENDA::P16250 (240 letters) >NCBI__GCF_900114975.1:WP_093393958.1 Length = 248 Score = 174 bits (440), Expect = 2e-48 Identities = 94/234 (40%), Positives = 141/234 (60%), Gaps = 3/234 (1%) Query: 6 LLPAVDVRDGQAVRLVHGESGTETSYGS-PLEAALAWQRSGAEWLHLVDLDAAF-GTGDN 63 + PA+D+++G+ VRL G+ ET YGS PL A W+ GA WLH+VDLD AF G + Sbjct: 3 IYPAIDIKNGKCVRLYQGDPSKETVYGSDPLTVARRWESLGATWLHIVDLDGAFAGRPVH 62 Query: 64 RALIAEVAQAMDIKVELSGGIRDDDTLAAALATGCTRVNLGTAALETPEWVAKVIAEHGD 123 +IA +A + I V++ GGIR+ + + L G +RV +GT AL+ PEW ++V + Sbjct: 63 YEVIARIASTLSIPVQVGGGIRNSEDVEKYLEAGISRVIVGTRALKDPEWFSQVCTAYPG 122 Query: 124 KIAVGLDVRGTTLRGRGWTRDGGDLYETLDRL-NKEGCARYVVTDIAKDGTLQGPNLELL 182 +IA GLD R + +GW + R+ N A + TDIA+DGT +G N++ Sbjct: 123 RIAAGLDARNGFVAVKGWKNVTSVTVAEVARIWNDLPLAALIYTDIARDGTQRGVNVKAT 182 Query: 183 KNVCAATDRPVVASGGVSSLDDLRAIAGLVPAGVEGAIVGKALYAKAFTLEEAL 236 +++ T PV+ASGGV+S++D+ + LV G+ G IVG+ALY + LEEA+ Sbjct: 183 ESLLKITRHPVIASGGVASIEDILVLLPLVKEGLNGVIVGRALYTGSLKLEEAI 236 Lambda K H 0.315 0.133 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 155 Number of extensions: 8 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 240 Length of database: 248 Length adjustment: 24 Effective length of query: 216 Effective length of database: 224 Effective search space: 48384 Effective search space used: 48384 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 46 (22.3 bits)
This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory