Alignments for a candidate for dapF in Kyrpidia tusciae DSM 2912

GapMind for Amino acid biosynthesis

Alignments for a candidate for dapF in Kyrpidia tusciae DSM 2912

Align Diaminopimelate epimerase; DAP epimerase; PLP-independent amino acid racemase; EC 5.1.1.7 (characterized)
to candidate WP_123809407.1 BTUS_RS07045 diaminopimelate epimerase

Query= SwissProt::Q81XR2
         (288 letters)



>NCBI__GCF_000092905.1:WP_123809407.1
          Length = 272

 Score =  242 bits (618), Expect = 6e-69
 Identities = 135/273 (49%), Positives = 168/273 (61%), Gaps = 8/273 (2%)

Query: 9   MHGLGNSYIYVNMFEEQIPEEDLALVAEKVSNINTGIGADGMILICPSDVAPVKMRMFNN 68
           MHGLGN +I V   E      D   +A    + + GIGADG++ + PSD A V+MR+FN 
Sbjct: 1   MHGLGNDFIVV---EGHALPLDPGALARNWCDRHFGIGADGLVFVLPSDRADVQMRIFNA 57

Query: 69  DGSEGKSCGNGLRCVAKYAYEHKLVEDTVFTIETLAGIVTAEVTVEEGKVTLAKIDMGAP 128
           DGSE + CGN +RCV KYA+E  LV      +ETLAG+    +     +V    +DMG P
Sbjct: 58  DGSEAEQCGNAVRCVGKYAFERGLVRRRDLVVETLAGVQRIVLGGNGSRVEEVTVDMGEP 117

Query: 129 RLTRAEIPM-LGEGETPFIRENFLYNNHRYAFTAVSMGNPHAVIFVDDVEQAPLTTLGPV 187
            L    IP+ L EG TP  R         +AFTAVSMGNPHAVIFVDDVE   +  +G  
Sbjct: 118 ILEPGRIPVALAEGGTPSGRVEAA--GREWAFTAVSMGNPHAVIFVDDVEGTDVRGVGSE 175

Query: 188 LETHEMFPERVNVEFIEILNEEEMNFRVWERGSGVTQACGTGACAAVVASILNGKMERGK 247
           +ET+ +FP RVNVEF+E L+  E+  RVWERG G T ACGTGACAAVVA  L G+   G+
Sbjct: 176 VETNRLFPNRVNVEFVETLDPGEIRVRVWERGCGETLACGTGACAAVVAGALEGR--SGR 233

Query: 248 EITVHLAGGDLMIAWTEEGNVLMKGPAEVICRG 280
           ++ VHL GG L I W E+G V M GPA  + RG
Sbjct: 234 KVRVHLPGGTLKIEWAEDGRVYMTGPAVEVFRG 266


Lambda     K      H
   0.318    0.136    0.398 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 256
Number of extensions: 16
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 288
Length of database: 272
Length adjustment: 26
Effective length of query: 262
Effective length of database: 246
Effective search space:    64452
Effective search space used:    64452
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 47 (22.7 bits)

Align candidate WP_123809407.1 BTUS_RS07045 (diaminopimelate epimerase)
to HMM TIGR00652 (dapF: diaminopimelate epimerase (EC 5.1.1.7))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00652.hmm
# target sequence database:        /tmp/gapView.16250.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00652  [M=270]
Accession:   TIGR00652
Description: DapF: diaminopimelate epimerase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
      1e-95  306.2   0.0    1.1e-95  306.0   0.0    1.0  1  lcl|NCBI__GCF_000092905.1:WP_123809407.1  BTUS_RS07045 diaminopimelate epi


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000092905.1:WP_123809407.1  BTUS_RS07045 diaminopimelate epimerase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  306.0   0.0   1.1e-95   1.1e-95       6     268 ..       1     268 [.       1     270 [. 0.93

  Alignments for each domain:
  == domain 1  score: 306.0 bits;  conditional E-value: 1.1e-95
                                 TIGR00652   6 mhGlgNdFvlvdevdeelvkeeaelvrkvcdrhtgvgaDgvllvepsseeadvklrifNsDGSeaemCG 74 
                                               mhGlgNdF++v+     l+    +l+r+ cdrh+g+gaDg+++v p s++adv++rifN+DGSeae+CG
  lcl|NCBI__GCF_000092905.1:WP_123809407.1   1 MHGLGNDFIVVEGHA--LPLDPGALARNWCDRHFGIGADGLVFVLP-SDRADVQMRIFNADGSEAEQCG 66 
                                               8***********998..5555579*********************8.********************** PP

                                 TIGR00652  75 NgiRcfakfvyekglkekkelsvetlaglikveveeen...kkvkvdmgepkfkkeeipltvekeeeke 140
                                               N++Rc+ k++ e+gl+++++l+vetlag+  + +  ++   ++v+vdmgep +++  ip+  ++  +++
  lcl|NCBI__GCF_000092905.1:WP_123809407.1  67 NAVRCVGKYAFERGLVRRRDLVVETLAGVQRIVLGGNGsrvEEVTVDMGEPILEPGRIPVALAEGGTPS 135
                                               ********************************99999998899******************65555544 PP

                                 TIGR00652 141 ellalev....l.vvdvGnPHlvvfvedvekldleelgklleaheefpegvNvefvevkkedeiklrvy 204
                                                 ++++     + +v++GnPH+v+fv+dve+ d+  +g+++e++  fp++vNvefve++++ ei++rv+
  lcl|NCBI__GCF_000092905.1:WP_123809407.1 136 GRVEAAGrewaFtAVSMGNPHAVIFVDDVEGTDVRGVGSEVETNRLFPNRVNVEFVETLDPGEIRVRVW 204
                                               3333333454427******************************************************** PP

                                 TIGR00652 205 ERGageTlaCGtGavAsavvalklgktkkkvtvhleggeLeievkedgkvyltGpavlvlegel 268
                                               ERG geTlaCGtGa+A++v+++  g++ +kv+vhl+gg L+ie+ edg+vy+tGpav v++ge+
  lcl|NCBI__GCF_000092905.1:WP_123809407.1 205 ERGCGETLACGTGACAAVVAGALEGRSGRKVRVHLPGGTLKIEWAEDGRVYMTGPAVEVFRGEI 268
                                               **************************************************************97 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (270 nodes)
Target sequences:                          1  (272 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.00
# Mc/sec: 8.90
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis