Alignments for a candidate for ilvC in Azohydromonas australica DSM 1124

GapMind for Amino acid biosynthesis

Alignments for a candidate for ilvC in Azohydromonas australica DSM 1124

Align acetohydroxyacid isomeroreductase (EC 1.1.1.86) (characterized)
to candidate WP_259375238.1 H537_RS0119910 ketol-acid reductoisomerase

Query= metacyc::MONOMER-18814
         (338 letters)



>NCBI__GCF_000430725.1:WP_259375238.1
          Length = 374

 Score =  583 bits (1502), Expect = e-171
 Identities = 288/338 (85%), Positives = 309/338 (91%)

Query: 1   MKVFYDKDADLSLIKGKNVTIIGYGSQGHAHALNLKDSGVNVTVGLRKSGASWNKAANAG 60
           M V+YDKDADLSLIKGKNVTIIGYGSQGHAHA NL DSGV VTVGLR+ GASW KA  AG
Sbjct: 37  MNVYYDKDADLSLIKGKNVTIIGYGSQGHAHAQNLNDSGVRVTVGLRRGGASWTKAEKAG 96

Query: 61  LQVKEVAEAVKGADVVMILLPDEQIADVYKNEVHDNIKEGAALAFAHGFNVHYGAVIPRA 120
           L+V E+A+AVK ADVVMILLPDEQIA VY  EV  N+K GA+LAFAHGFNVHYG V+PR 
Sbjct: 97  LKVAEIADAVKEADVVMILLPDEQIATVYNAEVAPNLKPGASLAFAHGFNVHYGQVVPRE 156

Query: 121 DLDVIMIAPKAPGHTVRATYTQGGGVPHLIAVHQNKSGAARDIALSYATANGGGRAGIIE 180
           D+DV M+APKAPGHTVR TYTQGGGVPHLIAV+Q+K+G ARD+ALSYA ANGGG+AGII+
Sbjct: 157 DVDVWMVAPKAPGHTVRYTYTQGGGVPHLIAVYQDKTGRARDLALSYAAANGGGKAGIIQ 216

Query: 181 TNFREETETDLFGEQAVLCGGTVELIKAGFETLVEAGYAPEMAYFECLHELKLIVDLIYE 240
           TNFREETETDLFGEQAVLCGGTVELIKAGFETLVEAGYAPEMAYFECLHELKLIVDLIYE
Sbjct: 217 TNFREETETDLFGEQAVLCGGTVELIKAGFETLVEAGYAPEMAYFECLHELKLIVDLIYE 276

Query: 241 GGIANMNYSISNNAEYGEYVTGPRVVTEETKKAMKQCLTDIQTGEYAKSFLLENKAGAPT 300
           GGI NMNYSISNNAEYGEYVTGPRVVTEETK AM+QCL DIQTGEYAKSF+LENKAGAPT
Sbjct: 277 GGIGNMNYSISNNAEYGEYVTGPRVVTEETKNAMRQCLKDIQTGEYAKSFILENKAGAPT 336

Query: 301 LISRRRLTAEHQIEEVGAKLRAMMPWIAKNKMVDQSKN 338
           LISRRRLTAEH IE+VG KLRAMMPWI  NKMVD+S+N
Sbjct: 337 LISRRRLTAEHPIEQVGEKLRAMMPWIKANKMVDKSRN 374


Lambda     K      H
   0.316    0.133    0.379 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 544
Number of extensions: 14
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 338
Length of database: 374
Length adjustment: 29
Effective length of query: 309
Effective length of database: 345
Effective search space:   106605
Effective search space used:   106605
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 49 (23.5 bits)

Align candidate WP_259375238.1 H537_RS0119910 (ketol-acid reductoisomerase)
to HMM TIGR00465 (ilvC: ketol-acid reductoisomerase (EC 1.1.1.86))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00465.hmm
# target sequence database:        /tmp/gapView.3693833.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00465  [M=314]
Accession:   TIGR00465
Description: ilvC: ketol-acid reductoisomerase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                             Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                             -----------
   2.9e-138  446.1   0.4   3.4e-138  445.9   0.4    1.0  1  NCBI__GCF_000430725.1:WP_259375238.1  


Domain annotation for each sequence (and alignments):
>> NCBI__GCF_000430725.1:WP_259375238.1  
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  445.9   0.4  3.4e-138  3.4e-138       1     313 [.      50     363 ..      50     364 .. 0.99

  Alignments for each domain:
  == domain 1  score: 445.9 bits;  conditional E-value: 3.4e-138
                             TIGR00465   1 lkgkkvaiiGyGsqGeaqalnlrdsglnvivglrkeaaswkkAeedGfkvltveeaikkadlimiLlpDevqk 73 
                                           +kgk+v+iiGyGsqG+a+a nl dsg+ v+vglr+++asw+kAe+ G+kv ++++a+k+ad++miLlpDe+  
  NCBI__GCF_000430725.1:WP_259375238.1  50 IKGKNVTIIGYGSQGHAHAQNLNDSGVRVTVGLRRGGASWTKAEKAGLKVAEIADAVKEADVVMILLPDEQIA 122
                                           689********************************************************************** PP

                             TIGR00465  74 evyeaeikpllkegkallfsHGfnivfkqivipkdvdvvlvAPKgpGalvReeykegrGvpsliAveqdvtge 146
                                           +vy+ae++p+lk g++l f+HGfn+++ q+v+++dvdv +vAPK+pG++vR +y +g Gvp+liAv+qd tg 
  NCBI__GCF_000430725.1:WP_259375238.1 123 TVYNAEVAPNLKPGASLAFAHGFNVHYGQVVPREDVDVWMVAPKAPGHTVRYTYTQGGGVPHLIAVYQDKTGR 195
                                           ************************************************************************* PP

                             TIGR00465 147 akeiAlayAkaiGgaragvlettFkeEvesDLfGEqavLcGglealikaafdtLveaGyqpelAyfeivhelk 219
                                           a++ Al+yA+a Gg++ag+++t F+eE+e+DLfGEqavLcGg  +lika+f+tLveaGy+pe+Ayfe++helk
  NCBI__GCF_000430725.1:WP_259375238.1 196 ARDLALSYAAANGGGKAGIIQTNFREETETDLFGEQAVLCGGTVELIKAGFETLVEAGYAPEMAYFECLHELK 268
                                           ************************************************************************* PP

                             TIGR00465 220 livdllkekGlelmrdavsntAklgalelr.eilkeelkkemqkilkeiqnGefakewalekeagkpafeear 291
                                           livdl++e+G+ +m  ++sn A++g++ ++ ++++ee+k++m++ lk+iq+Ge+ak ++le++ag+p++ + r
  NCBI__GCF_000430725.1:WP_259375238.1 269 LIVDLIYEGGIGNMNYSISNNAEYGEYVTGpRVVTEETKNAMRQCLKDIQTGEYAKSFILENKAGAPTLISRR 341
                                           ******************************9****************************************** PP

                             TIGR00465 292 kkekeqeiekvGkelralvkae 313
                                           +   e+ ie+vG++lra++++ 
  NCBI__GCF_000430725.1:WP_259375238.1 342 RLTAEHPIEQVGEKLRAMMPWI 363
                                           *******************986 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (314 nodes)
Target sequences:                          1  (374 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.01s 00:00:00.01 Elapsed: 00:00:00.00
# Mc/sec: 15.79
//
[ok]

This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis