Align acetylornithine deacetylase (EC 3.5.1.16) (characterized)
to candidate YP_001314282.1 Smed_5605 acetylornithine deacetylase
Query= BRENDA::Q92Y75 (374 letters) >NCBI__GCF_000017145.1:YP_001314282.1 Length = 374 Score = 689 bits (1777), Expect = 0.0 Identities = 345/374 (92%), Positives = 351/374 (93%) Query: 1 MQAAEILGKLVGFRSVVGLPNNDVVSWIRGYLESHGIAVDVLPGPEGDRSNIFATIGPKE 60 MQAAEILGKLVGFRSVVGLPN+D VSWIRGYLE HGIAVDVLPGPEGDRSNIFATIGPKE Sbjct: 1 MQAAEILGKLVGFRSVVGLPNDDAVSWIRGYLECHGIAVDVLPGPEGDRSNIFATIGPKE 60 Query: 61 ARGYIISGHMDVVPAAETGWTSDPFRLRVEADRLYGRGTTDMKGFLAAVLAAVPKLAAMP 120 A GYI+SGHMDVVPA E GWTSDPF LR E RLYGRGTTDMKGFLAA LAAVPKLAAMP Sbjct: 61 AHGYILSGHMDVVPATEAGWTSDPFILRAEGGRLYGRGTTDMKGFLAAGLAAVPKLAAMP 120 Query: 121 LRRPLHLALSYDEEAGCRGVPHMIARLPELCRQPLGAIIGEPTGMRAIRAHKGKAAARLT 180 LRRPLHLA SYDEEAGCRGVPHMIAR+PELCRQPLGAIIGEP+GMRAIRAHKGKAAARLT Sbjct: 121 LRRPLHLAFSYDEEAGCRGVPHMIARMPELCRQPLGAIIGEPSGMRAIRAHKGKAAARLT 180 Query: 181 VRGRSGHSSRPDQGLNAIHGVAGVLTQAVAEADRLVGGPFEHVFEPPYSSLQIGTVKGGQ 240 VRGRSGHSSRPDQGLNAIHGVAGVLTQAVAEA RL GGPFEHVFEPPYSSLQIGTVKGGQ Sbjct: 181 VRGRSGHSSRPDQGLNAIHGVAGVLTQAVAEAVRLAGGPFEHVFEPPYSSLQIGTVKGGQ 240 Query: 241 AVNIIPDSCEVEFEARAISGVDPAELLAPVRKTAEALTTLGFEVEWQELSAYPALSLEPD 300 AVNIIPDSCEVE EARAISGVDPAELL PVRK AEALT LGFEVEWQELSAYPALSLEP Sbjct: 241 AVNIIPDSCEVELEARAISGVDPAELLTPVRKIAEALTALGFEVEWQELSAYPALSLEPH 300 Query: 301 APLAALLEELTGREALPAVSYGTEAGLFQRAGIDAIICGPGDIGRAHKPDEYILIDELMA 360 APLAALLE LTGRE LPAVSYGTEAGLFQRAGIDAIICGPGDIGRAHK DEYILIDELMA Sbjct: 301 APLAALLEALTGREPLPAVSYGTEAGLFQRAGIDAIICGPGDIGRAHKADEYILIDELMA 360 Query: 361 CRAMVEALGARCTA 374 C+AM+EALGA C A Sbjct: 361 CQAMIEALGAHCIA 374 Lambda K H 0.319 0.137 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 592 Number of extensions: 16 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 374 Length of database: 374 Length adjustment: 30 Effective length of query: 344 Effective length of database: 344 Effective search space: 118336 Effective search space used: 118336 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 50 (23.9 bits)
Align candidate YP_001314282.1 Smed_5605 (acetylornithine deacetylase)
to HMM TIGR01892 (argE: acetylornithine deacetylase (ArgE) (EC 3.5.1.16))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR01892.hmm # target sequence database: /tmp/gapView.27957.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01892 [M=365] Accession: TIGR01892 Description: AcOrn-deacetyl: acetylornithine deacetylase (ArgE) Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1e-163 530.6 0.0 1.2e-163 530.4 0.0 1.0 1 lcl|NCBI__GCF_000017145.1:YP_001314282.1 Smed_5605 acetylornithine deacet Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000017145.1:YP_001314282.1 Smed_5605 acetylornithine deacetylase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 530.4 0.0 1.2e-163 1.2e-163 1 364 [. 5 368 .. 5 369 .. 0.99 Alignments for each domain: == domain 1 score: 530.4 bits; conditional E-value: 1.2e-163 TIGR01892 1 eilakLvafdsvsarsnvdlieyvedyleelgvavevlpvadgaeksnllaviGpkegagglvlsGhtD 69 eil+kLv+f+sv++++n d ++++++yle +g+av+vlp ++g ++sn++a+iGpke a g++lsGh+D lcl|NCBI__GCF_000017145.1:YP_001314282.1 5 EILGKLVGFRSVVGLPNDDAVSWIRGYLECHGIAVDVLPGPEG-DRSNIFATIGPKE-AHGYILSGHMD 71 79*****************************************.*************.*********** PP TIGR01892 70 vvPvdeaaWtsDpfrLtekdgrLYgrGtaDmkGFlalvLaavpdlaaakLkkPlhlvlsaDeevglaGa 138 vvP+ ea+WtsDpf L+ + grLYgrGt+DmkGFla+ Laavp+laa++L++Plhl++s+Dee+g++G+ lcl|NCBI__GCF_000017145.1:YP_001314282.1 72 VVPATEAGWTSDPFILRAEGGRLYGRGTTDMKGFLAAGLAAVPKLAAMPLRRPLHLAFSYDEEAGCRGV 140 ********************************************************************* PP TIGR01892 139 kklieala...rrpalaivGePtslvavRahkGkasakvtvrGreghssrpdrGvsaielaakllarlv 204 +++i++++ r+p ai+GeP +++a+RahkGka+a++tvrGr+ghssrpd+G++ai+ +a++l+++v lcl|NCBI__GCF_000017145.1:YP_001314282.1 141 PHMIARMPelcRQPLGAIIGEPSGMRAIRAHKGKAAARLTVRGRSGHSSRPDQGLNAIHGVAGVLTQAV 209 *******9999********************************************************** PP TIGR01892 205 aladklkredleeaFtppyatlniGtvkGGkavniiaaaCelvlelRpipGmdpeellallekiaeevk 273 a+a +l ++++e++F+ppy++l+iGtvkGG+avnii+++Ce++le+R+i+G+dp+ell++++kiae++ lcl|NCBI__GCF_000017145.1:YP_001314282.1 210 AEAVRLAGGPFEHVFEPPYSSLQIGTVKGGQAVNIIPDSCEVELEARAISGVDPAELLTPVRKIAEAL- 277 ******************************************************************76. PP TIGR01892 274 ekapgfevkveelsatpaleleedaelvalleklaGa.aaevvsygteagllqelGieavvlGPGdidq 341 +a+gfev+++elsa+pal+le+ a+l+alle l+G+ ++++vsygteagl+q++Gi+a+++GPGdi++ lcl|NCBI__GCF_000017145.1:YP_001314282.1 278 -TALGFEVEWQELSAYPALSLEPHAPLAALLEALTGRePLPAVSYGTEAGLFQRAGIDAIICGPGDIGR 345 .9***********************************99****************************** PP TIGR01892 342 ahqpdeYveieelkrcrallerl 364 ah++deY+ i+el++c+a++e+l lcl|NCBI__GCF_000017145.1:YP_001314282.1 346 AHKADEYILIDELMACQAMIEAL 368 ********************987 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (365 nodes) Target sequences: 1 (374 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01 # Mc/sec: 7.00 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory