Alignments for a candidate for metC in Sinorhizobium medicae WSM419

GapMind for Amino acid biosynthesis

Alignments for a candidate for metC in Sinorhizobium medicae WSM419

Align alanine racemase (EC 5.1.1.1) (characterized)
to candidate YP_001326795.1 Smed_1109 cystathionine beta-lyase

Query= BRENDA::P06721
         (395 letters)



>NCBI__GCF_000017145.1:YP_001326795.1
          Length = 469

 Score =  326 bits (835), Expect = 1e-93
 Identities = 180/394 (45%), Positives = 246/394 (62%), Gaps = 15/394 (3%)

Query: 2   ADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKHATRNRANGELFYGRRGT 61
           A++ ++T+L + G +     G VN  +  AS+++F +  A+   TR++   +  YG RGT
Sbjct: 83  AEQGINTRLAHTGNNPSDYHGFVNPPVVHASTVLFPN--ARTMETRSQ---KYTYGTRGT 137

Query: 62  LTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFIEQGDHVLMTNTAYEPSQDFCSKI 121
            T  +L +A+ ELEG AG +L P G AAV    LA++  GDHVL+ ++ Y P++ FC  +
Sbjct: 138 PTTDALCEAINELEGAAGTILVPSGLAAVTVPFLAYLSSGDHVLIVDSVYFPTRHFCDTM 197

Query: 122 LSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSITMEVHDVPAIVAAVRSVVPDAI 181
           L++LGVT  ++DP IGA I   ++PNT++V  E+PGS T E+ D+PAI AA        I
Sbjct: 198 LTRLGVTVEYYDPSIGAGIENLIRPNTRLVHTEAPGSNTFEMQDIPAIAAAAHR--HGCI 255

Query: 182 IMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDAMIGTAVCNARCWEQLRENAYLMG 241
           + +DNTWA  V F+ LD+G+DVSI AATKY  GHSD ++GT   NA  W  L E    +G
Sbjct: 256 VTMDNTWATPVYFRPLDYGVDVSIHAATKYPSGHSDVLLGTVSANAAHWPALSEAMVTLG 315

Query: 242 QMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEWLAEHPQVARVNHPALPGSKGHEFWK 301
             V  D +Y   RGLRT+GVRL +H  S+L +AEWL    +VARV HPALP   GHE WK
Sbjct: 316 VCVSPDDSYQILRGLRTMGVRLERHQASALAIAEWLEGREEVARVLHPALPSFPGHELWK 375

Query: 302 RDFTGSSGLFSFVLKKKLNN--EELANYLDNFSLFSMAYSWGGYESL-ILANQPEHIAAI 358
           RDF G+SG+FSFVLK    N   +   +LD  S+F + YSWGG+ESL +  N  +   A 
Sbjct: 376 RDFGGASGIFSFVLKADPENGKAKAHAFLDALSIFGLGYSWGGFESLAVHVNLSDRKVAK 435

Query: 359 RPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA 392
            PQ      G +IRL IGLEDV D+  D++AG A
Sbjct: 436 APQ-----EGPVIRLQIGLEDVPDIRRDVEAGLA 464


Lambda     K      H
   0.321    0.135    0.402 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 428
Number of extensions: 23
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 395
Length of database: 469
Length adjustment: 32
Effective length of query: 363
Effective length of database: 437
Effective search space:   158631
Effective search space used:   158631
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis